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On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3'-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies.
J Pharm Biomed Anal. 2013 Mar 25; 76:126-33.JP

Abstract

At the time of writing, this work appears to be the first published report on the coupling of on-line solid phase extraction (SPE) with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the simultaneous detection of nicotine, cotinine and trans-3'-hydroxycotinine in human urine. The advantages of both on-line SPE (speed, automation, less labor intensive) coupled with UPLC-MS/MS (speed, sensitivity) offer a viable option for efficient and economical biomonitoring studies for the assessment of active and passive exposure to tobacco smoke and clinical studies focusing on smoke cessation techniques. In a first approach, a 1:100 dilution of the urine was applied to screen for both passive and active exposure. Intra- and inter-batch reproducibility of this fast method (10min) was assessed for three concentration levels and were found to be less than 8% for each analyte while the accuracy was between 89 and 113%. To further improve the sensitivity of this approach when focusing only on passive smokers, the performance of a 1:10 dilution with the on-line SPE UPLC-MS/MS system was also tested. Results indeed show better sensitivity (LOQ's 1.0, 1.0 and 5.0μg/l for respectively cotinine, nicotine and trans-3'-hydroxycotinine) and good analytical performance for all other analytical parameters on the low levels tested here. Both methods were applied to measure the concentration of nicotine, cotinine and trans-3'-hydroxycotinine in the urine of 53 volunteers (smokers and non-smokers) recruited via an internal call at the Scientific Institute of Public Health. For non-smokers and smokers, cotinine levels e.g. were respectively between 1.0-470μg/l and 97-2381μg/l. Both these on-line SPE UPLC-MS/MS methods showed their potential for dedicated future large biomonitoring projects as they made it possible to analyze large series of samples in a fast, sensitive, robust and cost-efficient manner.

Authors+Show Affiliations

Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. koen.decremer@wiv-isp.beNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

23313774

Citation

De Cremer, Koen, et al. "On-line Solid-phase Extraction With Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry for the Detection of Nicotine, Cotinine and Trans-3'-hydroxycotinine in Urine to Strengthen Human Biomonitoring and Smoking Cessation Studies." Journal of Pharmaceutical and Biomedical Analysis, vol. 76, 2013, pp. 126-33.
De Cremer K, Van Overmeire I, Van Loco J. On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3'-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies. J Pharm Biomed Anal. 2013;76:126-33.
De Cremer, K., Van Overmeire, I., & Van Loco, J. (2013). On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3'-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies. Journal of Pharmaceutical and Biomedical Analysis, 76, 126-33. https://doi.org/10.1016/j.jpba.2012.12.018
De Cremer K, Van Overmeire I, Van Loco J. On-line Solid-phase Extraction With Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry for the Detection of Nicotine, Cotinine and Trans-3'-hydroxycotinine in Urine to Strengthen Human Biomonitoring and Smoking Cessation Studies. J Pharm Biomed Anal. 2013 Mar 25;76:126-33. PubMed PMID: 23313774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3'-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies. AU - De Cremer,Koen, AU - Van Overmeire,Ilse, AU - Van Loco,Joris, Y1 - 2012/12/21/ PY - 2012/07/13/received PY - 2012/10/31/revised PY - 2012/12/13/accepted PY - 2013/1/15/entrez PY - 2013/1/15/pubmed PY - 2013/7/10/medline SP - 126 EP - 33 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 76 N2 - At the time of writing, this work appears to be the first published report on the coupling of on-line solid phase extraction (SPE) with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the simultaneous detection of nicotine, cotinine and trans-3'-hydroxycotinine in human urine. The advantages of both on-line SPE (speed, automation, less labor intensive) coupled with UPLC-MS/MS (speed, sensitivity) offer a viable option for efficient and economical biomonitoring studies for the assessment of active and passive exposure to tobacco smoke and clinical studies focusing on smoke cessation techniques. In a first approach, a 1:100 dilution of the urine was applied to screen for both passive and active exposure. Intra- and inter-batch reproducibility of this fast method (10min) was assessed for three concentration levels and were found to be less than 8% for each analyte while the accuracy was between 89 and 113%. To further improve the sensitivity of this approach when focusing only on passive smokers, the performance of a 1:10 dilution with the on-line SPE UPLC-MS/MS system was also tested. Results indeed show better sensitivity (LOQ's 1.0, 1.0 and 5.0μg/l for respectively cotinine, nicotine and trans-3'-hydroxycotinine) and good analytical performance for all other analytical parameters on the low levels tested here. Both methods were applied to measure the concentration of nicotine, cotinine and trans-3'-hydroxycotinine in the urine of 53 volunteers (smokers and non-smokers) recruited via an internal call at the Scientific Institute of Public Health. For non-smokers and smokers, cotinine levels e.g. were respectively between 1.0-470μg/l and 97-2381μg/l. Both these on-line SPE UPLC-MS/MS methods showed their potential for dedicated future large biomonitoring projects as they made it possible to analyze large series of samples in a fast, sensitive, robust and cost-efficient manner. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/23313774/On_line_solid_phase_extraction_with_ultra_performance_liquid_chromatography_and_tandem_mass_spectrometry_for_the_detection_of_nicotine_cotinine_and_trans_3'_hydroxycotinine_in_urine_to_strengthen_human_biomonitoring_and_smoking_cessation_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(12)00690-5 DB - PRIME DP - Unbound Medicine ER -