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Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography-tandem mass spectrometry.
Anal Bioanal Chem. 2013 May; 405(12):3953-63.AB

Abstract

A method using microextraction by packed sorbent (MEPS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) is described for the determination of seven antipsychotic drugs in human plasma. The studied compounds were chlorpromazine (CPZ), haloperidol (HAL), cyamemazine, quetiapine, clozapine, olanzapine (OLZ), and levomepromazine; promazine, protriptyline, and deuterated CPZ were used as internal standards. The validation parameters included selectivity, linearity and limits of detection and quantitation, intra- and interday precision and trueness, recovery, and stability and were studied according to internationally accepted guidelines. The method was found to be linear between the lower limit of quantitation and 1000 ng/mL, except for OLZ and HAL (200 ng/mL), with determination coefficients higher than 0.99 for all analytes, and extraction efficiencies ranged from 62 to 92 %. Intra- and interday precision ranged from 0.24 to 10.67 %, while trueness was within a ±15 % interval from the nominal concentration for all analytes at all studied levels. MEPS has shown to be a rapid procedure for the determination of the selected antipsychotic drugs in human plasma, allowing reducing the handling time and the costs of analysis. Furthermore, GC-MS/MS has demonstrated to be a powerful tool for the simultaneous quantitation of the studied compounds, enabling obtaining adequate selectivity and sensitivity using a sample volume of as low as 0.25 mL.

Authors+Show Affiliations

CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

23314486

Citation

da Fonseca, B M., et al. "Determination of Seven Selected Antipsychotic Drugs in Human Plasma Using Microextraction in Packed Sorbent and Gas Chromatography-tandem Mass Spectrometry." Analytical and Bioanalytical Chemistry, vol. 405, no. 12, 2013, pp. 3953-63.
da Fonseca BM, Moreno IE, Barroso M, et al. Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2013;405(12):3953-63.
da Fonseca, B. M., Moreno, I. E., Barroso, M., Costa, S., Queiroz, J. A., & Gallardo, E. (2013). Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 405(12), 3953-63. https://doi.org/10.1007/s00216-012-6695-y
da Fonseca BM, et al. Determination of Seven Selected Antipsychotic Drugs in Human Plasma Using Microextraction in Packed Sorbent and Gas Chromatography-tandem Mass Spectrometry. Anal Bioanal Chem. 2013;405(12):3953-63. PubMed PMID: 23314486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography-tandem mass spectrometry. AU - da Fonseca,B M, AU - Moreno,I E D, AU - Barroso,M, AU - Costa,S, AU - Queiroz,J A, AU - Gallardo,E, Y1 - 2013/01/12/ PY - 2012/07/25/received PY - 2012/12/22/accepted PY - 2012/12/12/revised PY - 2013/1/15/entrez PY - 2013/1/15/pubmed PY - 2013/11/6/medline SP - 3953 EP - 63 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 405 IS - 12 N2 - A method using microextraction by packed sorbent (MEPS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) is described for the determination of seven antipsychotic drugs in human plasma. The studied compounds were chlorpromazine (CPZ), haloperidol (HAL), cyamemazine, quetiapine, clozapine, olanzapine (OLZ), and levomepromazine; promazine, protriptyline, and deuterated CPZ were used as internal standards. The validation parameters included selectivity, linearity and limits of detection and quantitation, intra- and interday precision and trueness, recovery, and stability and were studied according to internationally accepted guidelines. The method was found to be linear between the lower limit of quantitation and 1000 ng/mL, except for OLZ and HAL (200 ng/mL), with determination coefficients higher than 0.99 for all analytes, and extraction efficiencies ranged from 62 to 92 %. Intra- and interday precision ranged from 0.24 to 10.67 %, while trueness was within a ±15 % interval from the nominal concentration for all analytes at all studied levels. MEPS has shown to be a rapid procedure for the determination of the selected antipsychotic drugs in human plasma, allowing reducing the handling time and the costs of analysis. Furthermore, GC-MS/MS has demonstrated to be a powerful tool for the simultaneous quantitation of the studied compounds, enabling obtaining adequate selectivity and sensitivity using a sample volume of as low as 0.25 mL. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/23314486/Determination_of_seven_selected_antipsychotic_drugs_in_human_plasma_using_microextraction_in_packed_sorbent_and_gas_chromatography_tandem_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/s00216-012-6695-y DB - PRIME DP - Unbound Medicine ER -