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Effects of 17β-estradiol on turkey myogenic satellite cell proliferation, differentiation, and expression of glypican-1, MyoD and myogenin.
Comp Biochem Physiol A Mol Integr Physiol. 2013 Apr; 164(4):565-71.CB

Abstract

The hypothesis of this study was that 17β-estradiol (estradiol) stimulates turkey skeletal muscle growth by influencing myogenic satellite cell proliferation, differentiation, and the gene expression of selected proteins important in regulating growth and development. Increasing levels of estradiol were administered in basal medium containing additional nutrients. Female-derived pectoralis major (PM) satellite cell proliferation was stimulated by estradiol at a level of 10(-9)M following 4days of treatment. Male PM and biceps femoris (BF) satellite cell proliferation was increased at 10(-12)M estradiol. Turkey embryonic myoblast proliferation, however, decreased with 10(-9)M and 10(-5)M estradiol following 3days under these conditions. Estradiol had no effect on the differentiation of any of the 4 groups of cells. Likewise, glypican-1 expression was unaffected by estradiol treatment. MyoD expression decreased in male PM but not BF cells. MyoD expression in female PM cells and embryonic myoblasts were also unaffected by estradiol administration. Estradiol decreased myogenin expression in male satellite cells, but had no effect on female cells. There was a slight decrease in myogenin expression in embryonic myoblasts. The results demonstrate a direct effect of estradiol on avian satellite cell proliferation independent of glypican-1, and decreased expression of MyoD and myogenin in some myogenic cells, coinciding with increased cellular proliferation.

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Authors+Show Affiliations

McFarland DC
Department of Animal Science, South Dakota State University, Brookings, SD 57007, USA. Douglas.McFarland@sdstate.edu
Pesall JE
No affiliation info available
Coy CS
No affiliation info available
Velleman SG
No affiliation info available

MeSH

AnimalsCell DifferentiationCell ProliferationEstradiolFemaleGene ExpressionGlypicansMaleMuscle DevelopmentMyoD ProteinMyoblastsMyogeninPectoralis MusclesSatellite Cells, Skeletal MuscleTurkeys

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23319163

Citation

McFarland, Douglas C., et al. "Effects of 17β-estradiol On Turkey Myogenic Satellite Cell Proliferation, Differentiation, and Expression of Glypican-1, MyoD and Myogenin." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 164, no. 4, 2013, pp. 565-71.
McFarland DC, Pesall JE, Coy CS, et al. Effects of 17β-estradiol on turkey myogenic satellite cell proliferation, differentiation, and expression of glypican-1, MyoD and myogenin. Comp Biochem Physiol A Mol Integr Physiol. 2013;164(4):565-71.
McFarland, D. C., Pesall, J. E., Coy, C. S., & Velleman, S. G. (2013). Effects of 17β-estradiol on turkey myogenic satellite cell proliferation, differentiation, and expression of glypican-1, MyoD and myogenin. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 164(4), 565-71. https://doi.org/10.1016/j.cbpa.2013.01.001
McFarland DC, et al. Effects of 17β-estradiol On Turkey Myogenic Satellite Cell Proliferation, Differentiation, and Expression of Glypican-1, MyoD and Myogenin. Comp Biochem Physiol A Mol Integr Physiol. 2013;164(4):565-71. PubMed PMID: 23319163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of 17β-estradiol on turkey myogenic satellite cell proliferation, differentiation, and expression of glypican-1, MyoD and myogenin. AU - McFarland,Douglas C, AU - Pesall,Jane E, AU - Coy,Cynthia S, AU - Velleman,Sandra G, Y1 - 2013/01/11/ PY - 2012/08/09/received PY - 2012/12/28/revised PY - 2013/01/03/accepted PY - 2013/1/16/entrez PY - 2013/1/16/pubmed PY - 2014/1/15/medline SP - 565 EP - 71 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 164 IS - 4 N2 - The hypothesis of this study was that 17β-estradiol (estradiol) stimulates turkey skeletal muscle growth by influencing myogenic satellite cell proliferation, differentiation, and the gene expression of selected proteins important in regulating growth and development. Increasing levels of estradiol were administered in basal medium containing additional nutrients. Female-derived pectoralis major (PM) satellite cell proliferation was stimulated by estradiol at a level of 10(-9)M following 4days of treatment. Male PM and biceps femoris (BF) satellite cell proliferation was increased at 10(-12)M estradiol. Turkey embryonic myoblast proliferation, however, decreased with 10(-9)M and 10(-5)M estradiol following 3days under these conditions. Estradiol had no effect on the differentiation of any of the 4 groups of cells. Likewise, glypican-1 expression was unaffected by estradiol treatment. MyoD expression decreased in male PM but not BF cells. MyoD expression in female PM cells and embryonic myoblasts were also unaffected by estradiol administration. Estradiol decreased myogenin expression in male satellite cells, but had no effect on female cells. There was a slight decrease in myogenin expression in embryonic myoblasts. The results demonstrate a direct effect of estradiol on avian satellite cell proliferation independent of glypican-1, and decreased expression of MyoD and myogenin in some myogenic cells, coinciding with increased cellular proliferation. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/23319163/Effects_of_17β_estradiol_on_turkey_myogenic_satellite_cell_proliferation_differentiation_and_expression_of_glypican_1_MyoD_and_myogenin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(13)00002-0 DB - PRIME DP - Unbound Medicine ER -