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Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time.

Abstract

AIM

To investigate the impact of different time points of secondary warm ischemia on bile duct in a rat autologous liver transplantation model with external bile drainage.

METHODS

One hundred and thirty-six male inbred SD rats were randomly assigned to one of four groups (I-IV) according to the secondary warm ischemia time of 0, 10, 20 and 40 min. A rat model of autologous liver transplantation with continuous external biliary drainage under ether anesthesia was established. Ten rats in each group were used to evaluate the one-week survival rate. At 6 h, 24 h, 3 d and 7 d after reperfusion of the hepatic artery, 6 rats were killed in each group to collect the blood sample via the infrahepatic vena cava and the median lobe of liver for assay. Warm ischemia time of liver, cold perfusion time, anhepatic phase, operative duration for biliary external drainage and survival rates in the four groups were analyzed for the establishment of models.

RESULTS

No significant difference was shown in warm ischemia time, anhepatic phase and operative duration for biliary external drainage among the four groups. Five of the 40 rats in this study evaluated for the one-week survival rate died, including three deaths of severe pulmonary infection in group IV. A significant decrease of one-week survival rate in group IV was noted compared with the other three groups. With the prolongation of the biliary warm ischemia time, the indexes of the liver function assessment were significantly elevated, and biliary epithelial cell apoptosis index also increased. Pathological examinations showed significantly aggravated inflammation in the portal area and bile duct epithelial cell injury with the prolonged secondary warm ischemia time. Microthrombi were found in the micrangium around the biliary tract in some sections from groups III and IV.

CONCLUSION

The relationship between secondary warm ischemia time and the bile duct injury degree is time-dependent, and 20 min of secondary warm ischemia time is feasible for the study of bile duct injury.

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  • Authors+Show Affiliations

    ,

    Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, Jiangsu Province, China.

    , ,

    Source

    World journal of gastroenterology 18:48 2012 Dec 28 pg 7194-200

    MeSH

    Animals
    Apoptosis
    Bile Ducts
    Biliary Tract
    Disease Models, Animal
    Drainage
    Epithelial Cells
    Hepatic Artery
    Ischemia
    Liver
    Liver Function Tests
    Liver Transplantation
    Male
    Perfusion
    Rats
    Rats, Sprague-Dawley
    Time Factors
    Warm Ischemia

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23326124

    Citation

    Zhu, Xin-Hua, et al. "Establishment of a Rat Liver Transplantation Model With Prolonged Biliary Warm Ischemia Time." World Journal of Gastroenterology, vol. 18, no. 48, 2012, pp. 7194-200.
    Zhu XH, Pan JP, Wu YF, et al. Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time. World J Gastroenterol. 2012;18(48):7194-200.
    Zhu, X. H., Pan, J. P., Wu, Y. F., & Ding, Y. T. (2012). Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time. World Journal of Gastroenterology, 18(48), pp. 7194-200. doi:10.3748/wjg.v18.i48.7194.
    Zhu XH, et al. Establishment of a Rat Liver Transplantation Model With Prolonged Biliary Warm Ischemia Time. World J Gastroenterol. 2012 Dec 28;18(48):7194-200. PubMed PMID: 23326124.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time. AU - Zhu,Xin-Hua, AU - Pan,Jun-Ping, AU - Wu,Ya-Fu, AU - Ding,Yi-Tao, PY - 2012/09/11/received PY - 2012/10/30/revised PY - 2012/11/11/accepted PY - 2013/1/18/entrez PY - 2013/1/18/pubmed PY - 2014/1/10/medline KW - Bile duct KW - Liver KW - Rat KW - Transplantation KW - Warm ischemia SP - 7194 EP - 200 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 18 IS - 48 N2 - AIM: To investigate the impact of different time points of secondary warm ischemia on bile duct in a rat autologous liver transplantation model with external bile drainage. METHODS: One hundred and thirty-six male inbred SD rats were randomly assigned to one of four groups (I-IV) according to the secondary warm ischemia time of 0, 10, 20 and 40 min. A rat model of autologous liver transplantation with continuous external biliary drainage under ether anesthesia was established. Ten rats in each group were used to evaluate the one-week survival rate. At 6 h, 24 h, 3 d and 7 d after reperfusion of the hepatic artery, 6 rats were killed in each group to collect the blood sample via the infrahepatic vena cava and the median lobe of liver for assay. Warm ischemia time of liver, cold perfusion time, anhepatic phase, operative duration for biliary external drainage and survival rates in the four groups were analyzed for the establishment of models. RESULTS: No significant difference was shown in warm ischemia time, anhepatic phase and operative duration for biliary external drainage among the four groups. Five of the 40 rats in this study evaluated for the one-week survival rate died, including three deaths of severe pulmonary infection in group IV. A significant decrease of one-week survival rate in group IV was noted compared with the other three groups. With the prolongation of the biliary warm ischemia time, the indexes of the liver function assessment were significantly elevated, and biliary epithelial cell apoptosis index also increased. Pathological examinations showed significantly aggravated inflammation in the portal area and bile duct epithelial cell injury with the prolonged secondary warm ischemia time. Microthrombi were found in the micrangium around the biliary tract in some sections from groups III and IV. CONCLUSION: The relationship between secondary warm ischemia time and the bile duct injury degree is time-dependent, and 20 min of secondary warm ischemia time is feasible for the study of bile duct injury. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/23326124/Establishment_of_a_rat_liver_transplantation_model_with_prolonged_biliary_warm_ischemia_time_ L2 - http://www.wjgnet.com/1007-9327/full/v18/i48/7194.htm DB - PRIME DP - Unbound Medicine ER -