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A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae).
BMC Res Notes. 2013 Jan 19; 6:24.BR

Abstract

BACKGROUND

Antioxidants play an important role to protect damage caused by oxidative stress (OS). Plants having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties and phenolic contents (total phenols, flavonoids, flavonols and proanthrocyanidins) of methanolic extracts from Morus alba (locally named as Tut and commonly known as white mulberry) stem barks (TSB), root bark (TRB), leaves (TL) and fruits (TF) to make a statistical correlation between phenolic contents and antioxidant potential.

METHODS

The antioxidant activities and phenolic contents of methanolic extractives were evaluated by in vitro standard method using spectrophotometer. The antioxidant activities were determined by total antioxidant capacity, DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods.

RESULTS

Among the extracts, TSB showed the highest antioxidant activity followed by TRB, TF and TL. Based on DPPH and hydroxyl radical scavenging activity, the TSB extract was the most effective one with IC₅₀ 37.75 and 58.90 μg/mL, followed by TRB, TF and TL with IC₅₀ 40.20 and 102.03; 175.01 and 114.63 and 220.23 and 234.63 μg/mL, respectively. The TSB extract had the most potent inhibitory activity against lipid peroxidation with IC₅₀ 145.31 μg/mL. In addition, the reducing capacity on ferrous ion was in the following order: TSB > TRB > TL > TF. The content of phenolics, flavonoids, flavonols and proanthocyanidins of TSB was found to be higher than other extractives.

CONCLUSION

The results indicate high correlation and regression (p-value <0 .001) between phenolic contents and antioxidant potentials of the extracts, hence the Tut plant could serve as effective free radical inhibitor or scavenger which may be a good candidate for pharmaceutical plant-based products. However, further exploration is necessary for effective use in both modern and traditional system of medicines.

Authors+Show Affiliations

Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23331970

Citation

Khan, Muhammad Ali, et al. "A Comparative Study On the Antioxidant Activity of Methanolic Extracts From Different Parts of Morus Alba L. (Moraceae)." BMC Research Notes, vol. 6, 2013, p. 24.
Khan MA, Rahman AA, Islam S, et al. A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Res Notes. 2013;6:24.
Khan, M. A., Rahman, A. A., Islam, S., Khandokhar, P., Parvin, S., Islam, M. B., Hossain, M., Rashid, M., Sadik, G., Nasrin, S., Mollah, M. N., & Alam, A. H. (2013). A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Research Notes, 6, 24. https://doi.org/10.1186/1756-0500-6-24
Khan MA, et al. A Comparative Study On the Antioxidant Activity of Methanolic Extracts From Different Parts of Morus Alba L. (Moraceae). BMC Res Notes. 2013 Jan 19;6:24. PubMed PMID: 23331970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). AU - Khan,Muhammad Ali, AU - Rahman,Aziz Abdur, AU - Islam,Shafiqul, AU - Khandokhar,Proma, AU - Parvin,Shahnaj, AU - Islam,Md Badrul, AU - Hossain,Mosharrof, AU - Rashid,Mamunur, AU - Sadik,Golam, AU - Nasrin,Shamima, AU - Mollah,M Nurul Haque, AU - Alam,A H M Khurshid, Y1 - 2013/01/19/ PY - 2012/10/02/received PY - 2013/01/16/accepted PY - 2013/1/22/entrez PY - 2013/1/22/pubmed PY - 2013/6/26/medline SP - 24 EP - 24 JF - BMC research notes JO - BMC Res Notes VL - 6 N2 - BACKGROUND: Antioxidants play an important role to protect damage caused by oxidative stress (OS). Plants having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties and phenolic contents (total phenols, flavonoids, flavonols and proanthrocyanidins) of methanolic extracts from Morus alba (locally named as Tut and commonly known as white mulberry) stem barks (TSB), root bark (TRB), leaves (TL) and fruits (TF) to make a statistical correlation between phenolic contents and antioxidant potential. METHODS: The antioxidant activities and phenolic contents of methanolic extractives were evaluated by in vitro standard method using spectrophotometer. The antioxidant activities were determined by total antioxidant capacity, DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods. RESULTS: Among the extracts, TSB showed the highest antioxidant activity followed by TRB, TF and TL. Based on DPPH and hydroxyl radical scavenging activity, the TSB extract was the most effective one with IC₅₀ 37.75 and 58.90 μg/mL, followed by TRB, TF and TL with IC₅₀ 40.20 and 102.03; 175.01 and 114.63 and 220.23 and 234.63 μg/mL, respectively. The TSB extract had the most potent inhibitory activity against lipid peroxidation with IC₅₀ 145.31 μg/mL. In addition, the reducing capacity on ferrous ion was in the following order: TSB > TRB > TL > TF. The content of phenolics, flavonoids, flavonols and proanthocyanidins of TSB was found to be higher than other extractives. CONCLUSION: The results indicate high correlation and regression (p-value <0 .001) between phenolic contents and antioxidant potentials of the extracts, hence the Tut plant could serve as effective free radical inhibitor or scavenger which may be a good candidate for pharmaceutical plant-based products. However, further exploration is necessary for effective use in both modern and traditional system of medicines. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/23331970/A_comparative_study_on_the_antioxidant_activity_of_methanolic_extracts_from_different_parts_of_Morus_alba_L___Moraceae__ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-6-24 DB - PRIME DP - Unbound Medicine ER -