Tags

Type your tag names separated by a space and hit enter

Protective effect of lycopene against ochratoxin A induced renal oxidative stress and apoptosis in rats.
Exp Toxicol Pathol. 2013 Sep; 65(6):853-61.ET

Abstract

This study was designed to investigate the possible protective effect of lycopene against the renal toxic effects of OTA. Male Sprague-Dawley rats (<200 g, n=6) were treated with OTA (0.5 mg/kg/day) and/or lycopene (5 mg/kg/day) by gavage for 14 days. Histopathological examinations were performed and apoptotic cell death in both cortex and medulla was evaluated by TUNEL assay. Besides, biochemical parameters and activities of renal antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), thioredoxin reductase (TrxR)], catalase (CAT), superoxide dismutase (SOD); concentrations of total glutathione (GSH), and malondialdehyde (MDA) levels were measured. OTA treatment was found to induce oxidative stress in rat kidney, as evidenced by marked decreases in CAT (35%) activity and GSH levels (44%) as well as increase in SOD activity (22%) vs control group. Furthermore, TUNEL analysis revealed a significant increase in the number of TUNEL-positive cells in cortex (49%) and medulla (75%) in OTA administrated group compared to control (p<0.05). Lycopene supplementation with OTA increased GPx1 activity and GSH levels, and decreased apoptotic cell death in both cortex and medulla vs. control. The results of this study showed that at least one of the mechanisms underlying the renal toxicity of OTA is oxidative stress and apoptosis is the major form of cell death caused by OTA. Besides, our data indicate that the natural antioxidant lycopene might be partially protective against OTA-induced nephrotoxicity and oxidative stress in rat.

Authors+Show Affiliations

Hacettepe University, Faculty of Pharmacy, Department of Toxicology, 06100 Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23332503

Citation

Palabiyik, S Sezin, et al. "Protective Effect of Lycopene Against Ochratoxin a Induced Renal Oxidative Stress and Apoptosis in Rats." Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, vol. 65, no. 6, 2013, pp. 853-61.
Palabiyik SS, Erkekoglu P, Zeybek ND, et al. Protective effect of lycopene against ochratoxin A induced renal oxidative stress and apoptosis in rats. Exp Toxicol Pathol. 2013;65(6):853-61.
Palabiyik, S. S., Erkekoglu, P., Zeybek, N. D., Kizilgun, M., Baydar, D. E., Sahin, G., & Giray, B. K. (2013). Protective effect of lycopene against ochratoxin A induced renal oxidative stress and apoptosis in rats. Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, 65(6), 853-61. https://doi.org/10.1016/j.etp.2012.12.004
Palabiyik SS, et al. Protective Effect of Lycopene Against Ochratoxin a Induced Renal Oxidative Stress and Apoptosis in Rats. Exp Toxicol Pathol. 2013;65(6):853-61. PubMed PMID: 23332503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of lycopene against ochratoxin A induced renal oxidative stress and apoptosis in rats. AU - Palabiyik,S Sezin, AU - Erkekoglu,Pinar, AU - Zeybek,N Dilara, AU - Kizilgun,Murat, AU - Baydar,Dilek Ertoy, AU - Sahin,Gonul, AU - Giray,Belma Kocer, Y1 - 2013/01/15/ PY - 2012/09/10/received PY - 2012/11/27/revised PY - 2012/12/14/accepted PY - 2013/1/22/entrez PY - 2013/1/22/pubmed PY - 2014/2/14/medline KW - (1)O(2) KW - 5,5′-dithiobis-(2-nitrobenzoic) acid KW - 5-thio-2-nitrobenzoic acid KW - Antioxidant enzymes KW - Apoptosis KW - BEN KW - BUN KW - Balkan endemic nephropathy KW - CAT KW - DTNB KW - DTPA KW - GPx1 KW - GR KW - GSH KW - GSSG KW - HPLC KW - Kidney KW - LP KW - Lipid peroxidation KW - MDA KW - NADP(+) KW - NADPH KW - OTA KW - Ochratoxin A KW - Oxidative stress KW - PMSF KW - ROS KW - SD KW - SEM KW - SOD KW - SPSS KW - Sprague Dawley KW - Statistical Package for Social Sciences Program KW - TNB KW - TUNEL KW - TdT KW - TrxR KW - blood urea nitrogen KW - catalase KW - diethylenetriamine pentaacetic acid KW - glutathione peroxidase 1 KW - glutathione reductase KW - high performance liquid chromatography KW - lipid peroxidation KW - malondialdehyde KW - nicotinamide adenine dinucleotide phosphate KW - nicotinamide adenine dinucleotide phosphate, reduced form KW - ochratoxin A KW - oxidized glutathione KW - phenylmethanesulphonyl fluoride KW - reactive oxygen species KW - singlet oxygen KW - standard error of mean KW - superoxide dismutase KW - terminal deoxynucleotidyl transferase KW - terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling KW - thioredoxin reductase KW - total glutathione SP - 853 EP - 61 JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JO - Exp Toxicol Pathol VL - 65 IS - 6 N2 - This study was designed to investigate the possible protective effect of lycopene against the renal toxic effects of OTA. Male Sprague-Dawley rats (<200 g, n=6) were treated with OTA (0.5 mg/kg/day) and/or lycopene (5 mg/kg/day) by gavage for 14 days. Histopathological examinations were performed and apoptotic cell death in both cortex and medulla was evaluated by TUNEL assay. Besides, biochemical parameters and activities of renal antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), thioredoxin reductase (TrxR)], catalase (CAT), superoxide dismutase (SOD); concentrations of total glutathione (GSH), and malondialdehyde (MDA) levels were measured. OTA treatment was found to induce oxidative stress in rat kidney, as evidenced by marked decreases in CAT (35%) activity and GSH levels (44%) as well as increase in SOD activity (22%) vs control group. Furthermore, TUNEL analysis revealed a significant increase in the number of TUNEL-positive cells in cortex (49%) and medulla (75%) in OTA administrated group compared to control (p<0.05). Lycopene supplementation with OTA increased GPx1 activity and GSH levels, and decreased apoptotic cell death in both cortex and medulla vs. control. The results of this study showed that at least one of the mechanisms underlying the renal toxicity of OTA is oxidative stress and apoptosis is the major form of cell death caused by OTA. Besides, our data indicate that the natural antioxidant lycopene might be partially protective against OTA-induced nephrotoxicity and oxidative stress in rat. SN - 1618-1433 UR - https://www.unboundmedicine.com/medline/citation/23332503/Protective_effect_of_lycopene_against_ochratoxin_A_induced_renal_oxidative_stress_and_apoptosis_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0940-2993(12)00148-0 DB - PRIME DP - Unbound Medicine ER -