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DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate.
J Nutr Biochem. 2013 Jul; 24(7):1295-301.JN

Abstract

The proliferative response of neural stem cells (NSCs) to folate may play a critical role in the development, function and repair of the central nervous system. It is important to determine the dose-dependent effects of folate in NSC cultures that are potential sources of transplantable cells for therapies for neurodegenerative diseases. To determine the optimal concentration and mechanism of action of folate for stimulation of NSC proliferation in vitro, NSCs were exposed to folic acid or 5-methyltetrahydrofolate (5-MTHF) (0-200 μmol/L) for 24, 48 or 72 h. Immunocytochemistry and methyl thiazolyl tetrazolium assay showed that the optimal concentration of folic acid for NSC proliferation was 20-40 μmol/L. Stimulation of NSC proliferation by folic acid was associated with DNA methyltransferase (DNMT) activation and was attenuated by the DNMT inhibitor zebularine, which implies that folate dose-dependently stimulates NSC proliferation through a DNMT-dependent mechanism. Based on these new findings and previously published evidence, we have identified a mechanism by which folate stimulates NSC growth.

Authors+Show Affiliations

Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23332600

Citation

Li, Wen, et al. "DNA Methyltransferase Mediates Dose-dependent Stimulation of Neural Stem Cell Proliferation By Folate." The Journal of Nutritional Biochemistry, vol. 24, no. 7, 2013, pp. 1295-301.
Li W, Yu M, Luo S, et al. DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate. J Nutr Biochem. 2013;24(7):1295-301.
Li, W., Yu, M., Luo, S., Liu, H., Gao, Y., Wilson, J. X., & Huang, G. (2013). DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate. The Journal of Nutritional Biochemistry, 24(7), 1295-301. https://doi.org/10.1016/j.jnutbio.2012.11.001
Li W, et al. DNA Methyltransferase Mediates Dose-dependent Stimulation of Neural Stem Cell Proliferation By Folate. J Nutr Biochem. 2013;24(7):1295-301. PubMed PMID: 23332600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate. AU - Li,Wen, AU - Yu,Min, AU - Luo,Suhui, AU - Liu,Huan, AU - Gao,Yuxia, AU - Wilson,John X, AU - Huang,Guowei, Y1 - 2013/01/15/ PY - 2012/08/05/received PY - 2012/10/31/revised PY - 2012/11/09/accepted PY - 2013/1/22/entrez PY - 2013/1/22/pubmed PY - 2014/1/30/medline SP - 1295 EP - 301 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 24 IS - 7 N2 - The proliferative response of neural stem cells (NSCs) to folate may play a critical role in the development, function and repair of the central nervous system. It is important to determine the dose-dependent effects of folate in NSC cultures that are potential sources of transplantable cells for therapies for neurodegenerative diseases. To determine the optimal concentration and mechanism of action of folate for stimulation of NSC proliferation in vitro, NSCs were exposed to folic acid or 5-methyltetrahydrofolate (5-MTHF) (0-200 μmol/L) for 24, 48 or 72 h. Immunocytochemistry and methyl thiazolyl tetrazolium assay showed that the optimal concentration of folic acid for NSC proliferation was 20-40 μmol/L. Stimulation of NSC proliferation by folic acid was associated with DNA methyltransferase (DNMT) activation and was attenuated by the DNMT inhibitor zebularine, which implies that folate dose-dependently stimulates NSC proliferation through a DNMT-dependent mechanism. Based on these new findings and previously published evidence, we have identified a mechanism by which folate stimulates NSC growth. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/23332600/DNA_methyltransferase_mediates_dose_dependent_stimulation_of_neural_stem_cell_proliferation_by_folate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(12)00281-1 DB - PRIME DP - Unbound Medicine ER -