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Utility, reliability and reproducibility of immunoassay multiplex kits.
Methods. 2013 May 15; 61(1):23-9.M

Abstract

Multiplex technologies are becoming increasingly important in biomarker studies as they enable patterns of biomolecules to be examined, which provide a more comprehensive depiction of disease than individual biomarkers. They are crucial in deciphering these patterns, but it is essential that they are endorsed for reliability, reproducibility and precision. Here we outline the theoretical basis of a variety of multiplex technologies: Bead-based multiplex immunoassays (i.e. Cytometric Bead Arrays, Luminex™ and Bio-Plex Pro™), microtitre plate-based arrays (i.e. Mesoscale Discovery (MSD) and Quantsys BioSciences QPlex), Slide-based Arrays (i.e. FastQuant™) and reverse phase protein arrays. Their utility, reliability and reproducibility are discussed.

Authors+Show Affiliations

COPD Research Group, A Floor, West Block, Queens Medical Centre, The University of Nottingham, Nottingham NG7 2UH, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23333412

Citation

Tighe, Paddy, et al. "Utility, Reliability and Reproducibility of Immunoassay Multiplex Kits." Methods (San Diego, Calif.), vol. 61, no. 1, 2013, pp. 23-9.
Tighe P, Negm O, Todd I, et al. Utility, reliability and reproducibility of immunoassay multiplex kits. Methods. 2013;61(1):23-9.
Tighe, P., Negm, O., Todd, I., & Fairclough, L. (2013). Utility, reliability and reproducibility of immunoassay multiplex kits. Methods (San Diego, Calif.), 61(1), 23-9. https://doi.org/10.1016/j.ymeth.2013.01.003
Tighe P, et al. Utility, Reliability and Reproducibility of Immunoassay Multiplex Kits. Methods. 2013 May 15;61(1):23-9. PubMed PMID: 23333412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Utility, reliability and reproducibility of immunoassay multiplex kits. AU - Tighe,Paddy, AU - Negm,Ola, AU - Todd,Ian, AU - Fairclough,Lucy, Y1 - 2013/01/16/ PY - 2012/08/09/received PY - 2013/01/07/accepted PY - 2013/1/22/entrez PY - 2013/1/22/pubmed PY - 2014/1/8/medline SP - 23 EP - 9 JF - Methods (San Diego, Calif.) JO - Methods VL - 61 IS - 1 N2 - Multiplex technologies are becoming increasingly important in biomarker studies as they enable patterns of biomolecules to be examined, which provide a more comprehensive depiction of disease than individual biomarkers. They are crucial in deciphering these patterns, but it is essential that they are endorsed for reliability, reproducibility and precision. Here we outline the theoretical basis of a variety of multiplex technologies: Bead-based multiplex immunoassays (i.e. Cytometric Bead Arrays, Luminex™ and Bio-Plex Pro™), microtitre plate-based arrays (i.e. Mesoscale Discovery (MSD) and Quantsys BioSciences QPlex), Slide-based Arrays (i.e. FastQuant™) and reverse phase protein arrays. Their utility, reliability and reproducibility are discussed. SN - 1095-9130 UR - https://www.unboundmedicine.com/medline/citation/23333412/Utility_reliability_and_reproducibility_of_immunoassay_multiplex_kits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1046-2023(13)00014-5 DB - PRIME DP - Unbound Medicine ER -