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Cartilage regeneration by selected chondrogenic clonal mesenchymal stem cells in the collagenase-induced monkey osteoarthritis model.
J Tissue Eng Regen Med. 2014 Nov; 8(11):896-905.JT

Abstract

Osteoarthritis (OA) is the most common form of arthritis, in which cartilage is irreversibly degraded, causing severe pain and disability. Current therapeutic strategies cannot repair damaged cartilage. We evaluated the repair potential of selected chondrogenic clonal MSCs (sC-MSCs) by delivering them into the injured cartilage site in a collagenase-induced OA model in Cynomolgus monkeys. In vitro characterization showed that the isolated monkey sC-MSCs and polyclonal MSCs (P-MSCs) expressed mesenchymal stem cell markers and could differentiate into chondrocytes. The articular cartilage lesions in animals were treated with normal saline (NS), autologous P-MSCs and sC-MSCs, respectively, by direct delivery. The clinical parameters, radiographic images, histological and immunohistochemical examinations at weeks 8, 16 and 24 post-treatment demonstrated that the abrasions of articular cartilage were significantly improved and repaired by MSC-based treatment, particularly in the sC-MSC-treated group, which displayed consistently higher histological scores than those of other groups. In summary, treatment with sC-MSCs can effectively improve the healing of cartilage lesions in the Cynomolgus monkey collagenase-induced OA model. Due to the genetic proximity of monkey and human, the therapeutic strategy presented in this study will have broad applications in clinical practice.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jiang L
Department of Surgery, CRCHUM, Notre Dame Hospital, University of Montreal, Quebec, Canada; Department of Orthopaedics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
Ma A
No affiliation info available
Song L
No affiliation info available
Hu Y
No affiliation info available
Dun H
No affiliation info available
Daloze P
No affiliation info available
Yu Y
No affiliation info available
Jiang J
No affiliation info available
Zafarullah M
No affiliation info available
Chen H
No affiliation info available

MeSH

AnimalsCartilageChondrogenesisClone CellsCollagenasesDisease Models, AnimalHumansJointsMacaca fascicularisMaleMatrix MetalloproteinasesMesenchymal Stem Cell TransplantationMesenchymal Stem CellsOsteoarthritisRegenerationWound Healing

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23335439

Citation

Jiang, Li, et al. "Cartilage Regeneration By Selected Chondrogenic Clonal Mesenchymal Stem Cells in the Collagenase-induced Monkey Osteoarthritis Model." Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 11, 2014, pp. 896-905.
Jiang L, Ma A, Song L, et al. Cartilage regeneration by selected chondrogenic clonal mesenchymal stem cells in the collagenase-induced monkey osteoarthritis model. J Tissue Eng Regen Med. 2014;8(11):896-905.
Jiang, L., Ma, A., Song, L., Hu, Y., Dun, H., Daloze, P., Yu, Y., Jiang, J., Zafarullah, M., & Chen, H. (2014). Cartilage regeneration by selected chondrogenic clonal mesenchymal stem cells in the collagenase-induced monkey osteoarthritis model. Journal of Tissue Engineering and Regenerative Medicine, 8(11), 896-905. https://doi.org/10.1002/term.1676
Jiang L, et al. Cartilage Regeneration By Selected Chondrogenic Clonal Mesenchymal Stem Cells in the Collagenase-induced Monkey Osteoarthritis Model. J Tissue Eng Regen Med. 2014;8(11):896-905. PubMed PMID: 23335439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cartilage regeneration by selected chondrogenic clonal mesenchymal stem cells in the collagenase-induced monkey osteoarthritis model. AU - Jiang,Li, AU - Ma,Anlun, AU - Song,Lijun, AU - Hu,Yanxin, AU - Dun,Hao, AU - Daloze,Pierre, AU - Yu,Yonglin, AU - Jiang,Jianyuan, AU - Zafarullah,Muhammad, AU - Chen,Huifang, Y1 - 2013/01/21/ PY - 2012/04/26/received PY - 2012/09/11/revised PY - 2012/11/05/accepted PY - 2013/1/22/entrez PY - 2013/1/22/pubmed PY - 2015/6/25/medline KW - cartilage regeneration KW - collagenase-induced KW - mesenchymal stem cells KW - monkey KW - osteoarthritis KW - selected chondrogenic clonal SP - 896 EP - 905 JF - Journal of tissue engineering and regenerative medicine JO - J Tissue Eng Regen Med VL - 8 IS - 11 N2 - Osteoarthritis (OA) is the most common form of arthritis, in which cartilage is irreversibly degraded, causing severe pain and disability. Current therapeutic strategies cannot repair damaged cartilage. We evaluated the repair potential of selected chondrogenic clonal MSCs (sC-MSCs) by delivering them into the injured cartilage site in a collagenase-induced OA model in Cynomolgus monkeys. In vitro characterization showed that the isolated monkey sC-MSCs and polyclonal MSCs (P-MSCs) expressed mesenchymal stem cell markers and could differentiate into chondrocytes. The articular cartilage lesions in animals were treated with normal saline (NS), autologous P-MSCs and sC-MSCs, respectively, by direct delivery. The clinical parameters, radiographic images, histological and immunohistochemical examinations at weeks 8, 16 and 24 post-treatment demonstrated that the abrasions of articular cartilage were significantly improved and repaired by MSC-based treatment, particularly in the sC-MSC-treated group, which displayed consistently higher histological scores than those of other groups. In summary, treatment with sC-MSCs can effectively improve the healing of cartilage lesions in the Cynomolgus monkey collagenase-induced OA model. Due to the genetic proximity of monkey and human, the therapeutic strategy presented in this study will have broad applications in clinical practice. SN - 1932-7005 UR - https://www.unboundmedicine.com/medline/citation/23335439/Cartilage_regeneration_by_selected_chondrogenic_clonal_mesenchymal_stem_cells_in_the_collagenase_induced_monkey_osteoarthritis_model_ L2 - https://doi.org/10.1002/term.1676 DB - PRIME DP - Unbound Medicine ER -