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An ultrasensitive electrochemical aptasensor for thrombin based on the triplex-amplification of hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme and horseradish peroxidase decorated FeTe nanorods.
Analyst. 2013 Mar 07; 138(5):1497-503.A

Abstract

In the present study, we fabricated an ultrasensitive sandwich-type electrochemical aptasensor for thrombin (TB) based on a triplex signal amplification strategy. The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) as well as blocking reagent-horseradish peroxidase (HRP) and iron telluride nanorods (FeTe NRs) could simultaneously amplify the electrochemical signal of thionine (Thi) in the presence of H(2)O(2). Herein, FeTe NRs, as a newly discovered HRP-mimicking enzyme, were employed to construct an aptasensor for the first time. And, the FeTe NRs decorated by gold nanoparticles (abbreviated as AuNPs@FeTe NRs), were not only used as carriers of secondary thrombin aptamer (TBA 2), electron mediator thionine (Thi) and HRP, but also catalyzed the electrochemical reaction of Thi in the presence of H(2)O(2). As can be seen from experiment results, with the triplex signal amplification strategy, the reduction peak current of the fabricated aptasensor was logarithmically related to the concentration of thrombin (TB) over a wide range from 1 pM to 20 nM, and a detection limit of 0.5 pM was obtained. Hence, the proposed aptamer-based sandwich sensing approach for amplified detection of TB could provide a promising way for highly sensitive determination of other analytes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jiang L
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Yuan R
No affiliation info available
Chai Y
No affiliation info available
Yuan Y
No affiliation info available
Bai L
No affiliation info available
Wang Y
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesDNA, CatalyticElectrochemical TechniquesEnzymes, ImmobilizedG-QuadruplexesGoldHeminHorseradish PeroxidaseHumansIron CompoundsLimit of DetectionMetal NanoparticlesNanotubesTelluriumThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23340527

Citation

Jiang, Liping, et al. "An Ultrasensitive Electrochemical Aptasensor for Thrombin Based On the Triplex-amplification of hemin/G-quadruplex Horseradish Peroxidase-mimicking DNAzyme and Horseradish Peroxidase Decorated FeTe Nanorods." The Analyst, vol. 138, no. 5, 2013, pp. 1497-503.
Jiang L, Yuan R, Chai Y, et al. An ultrasensitive electrochemical aptasensor for thrombin based on the triplex-amplification of hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme and horseradish peroxidase decorated FeTe nanorods. Analyst. 2013;138(5):1497-503.
Jiang, L., Yuan, R., Chai, Y., Yuan, Y., Bai, L., & Wang, Y. (2013). An ultrasensitive electrochemical aptasensor for thrombin based on the triplex-amplification of hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme and horseradish peroxidase decorated FeTe nanorods. The Analyst, 138(5), 1497-503. https://doi.org/10.1039/c2an36772f
Jiang L, et al. An Ultrasensitive Electrochemical Aptasensor for Thrombin Based On the Triplex-amplification of hemin/G-quadruplex Horseradish Peroxidase-mimicking DNAzyme and Horseradish Peroxidase Decorated FeTe Nanorods. Analyst. 2013 Mar 7;138(5):1497-503. PubMed PMID: 23340527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An ultrasensitive electrochemical aptasensor for thrombin based on the triplex-amplification of hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme and horseradish peroxidase decorated FeTe nanorods. AU - Jiang,Liping, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Yuan,Yali, AU - Bai,Lijuan, AU - Wang,Yan, PY - 2013/1/24/entrez PY - 2013/1/24/pubmed PY - 2013/12/16/medline SP - 1497 EP - 503 JF - The Analyst JO - Analyst VL - 138 IS - 5 N2 - In the present study, we fabricated an ultrasensitive sandwich-type electrochemical aptasensor for thrombin (TB) based on a triplex signal amplification strategy. The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) as well as blocking reagent-horseradish peroxidase (HRP) and iron telluride nanorods (FeTe NRs) could simultaneously amplify the electrochemical signal of thionine (Thi) in the presence of H(2)O(2). Herein, FeTe NRs, as a newly discovered HRP-mimicking enzyme, were employed to construct an aptasensor for the first time. And, the FeTe NRs decorated by gold nanoparticles (abbreviated as AuNPs@FeTe NRs), were not only used as carriers of secondary thrombin aptamer (TBA 2), electron mediator thionine (Thi) and HRP, but also catalyzed the electrochemical reaction of Thi in the presence of H(2)O(2). As can be seen from experiment results, with the triplex signal amplification strategy, the reduction peak current of the fabricated aptasensor was logarithmically related to the concentration of thrombin (TB) over a wide range from 1 pM to 20 nM, and a detection limit of 0.5 pM was obtained. Hence, the proposed aptamer-based sandwich sensing approach for amplified detection of TB could provide a promising way for highly sensitive determination of other analytes. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/23340527/An_ultrasensitive_electrochemical_aptasensor_for_thrombin_based_on_the_triplex_amplification_of_hemin/G_quadruplex_horseradish_peroxidase_mimicking_DNAzyme_and_horseradish_peroxidase_decorated_FeTe_nanorods_ DB - PRIME DP - Unbound Medicine ER -