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SlARF4, an auxin response factor involved in the control of sugar metabolism during tomato fruit development.
Plant Physiol. 2013 Mar; 161(3):1362-74.PP

Abstract

Successful completion of fruit developmental programs depends on the interplay between multiple phytohormones. However, besides ethylene, the impact of other hormones on fruit quality traits remains elusive. A previous study has shown that down-regulation of SlARF4, a member of the tomato (Solanum lycopersicum) auxin response factor (ARF) gene family, results in a dark-green fruit phenotype with increased chloroplasts (Jones et al., 2002). This study further examines the role of this auxin transcriptional regulator during tomato fruit development at the level of transcripts, enzyme activities, and metabolites. It is noteworthy that the dark-green phenotype of antisense SlARF4-suppressed lines is restricted to fruit, suggesting that SlARF4 controls chlorophyll accumulation specifically in this organ. The SlARF4 underexpressing lines accumulate more starch at early stages of fruit development and display enhanced chlorophyll content and photochemical efficiency, which is consistent with the idea that fruit photosynthetic activity accounts for the elevated starch levels. SlARF4 expression is high in pericarp tissues of immature fruit and then undergoes a dramatic decline at the onset of ripening concomitant with the increase in sugar content. The higher starch content in developing fruits of SlARF4 down-regulated lines correlates with the up-regulation of genes and enzyme activities involved in starch biosynthesis, suggesting their negative regulation by SlARF4. Altogether, the data uncover the involvement of ARFs in the control of sugar content, an essential feature of fruit quality, and provide insight into the link between auxin signaling, chloroplastic activity, and sugar metabolism in developing fruit.

Authors+Show Affiliations

Université de Toulouse, Institut National Polytechnique-Ecole Nationale Supérieure Agronomique de Toulouse, Laboratoire de Génomique et Biotechnologie des Fruits, F-31326 Castanet-Tolosan, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23341361

Citation

Sagar, Maha, et al. "SlARF4, an Auxin Response Factor Involved in the Control of Sugar Metabolism During Tomato Fruit Development." Plant Physiology, vol. 161, no. 3, 2013, pp. 1362-74.
Sagar M, Chervin C, Mila I, et al. SlARF4, an auxin response factor involved in the control of sugar metabolism during tomato fruit development. Plant Physiol. 2013;161(3):1362-74.
Sagar, M., Chervin, C., Mila, I., Hao, Y., Roustan, J. P., Benichou, M., Gibon, Y., Biais, B., Maury, P., Latché, A., Pech, J. C., Bouzayen, M., & Zouine, M. (2013). SlARF4, an auxin response factor involved in the control of sugar metabolism during tomato fruit development. Plant Physiology, 161(3), 1362-74. https://doi.org/10.1104/pp.113.213843
Sagar M, et al. SlARF4, an Auxin Response Factor Involved in the Control of Sugar Metabolism During Tomato Fruit Development. Plant Physiol. 2013;161(3):1362-74. PubMed PMID: 23341361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SlARF4, an auxin response factor involved in the control of sugar metabolism during tomato fruit development. AU - Sagar,Maha, AU - Chervin,Christian, AU - Mila,Isabelle, AU - Hao,Yanwei, AU - Roustan,Jean-Paul, AU - Benichou,Mohamed, AU - Gibon,Yves, AU - Biais,Benoît, AU - Maury,Pierre, AU - Latché,Alain, AU - Pech,Jean-Claude, AU - Bouzayen,Mondher, AU - Zouine,Mohamed, Y1 - 2013/01/22/ PY - 2013/1/24/entrez PY - 2013/1/24/pubmed PY - 2013/8/16/medline SP - 1362 EP - 74 JF - Plant physiology JO - Plant Physiol VL - 161 IS - 3 N2 - Successful completion of fruit developmental programs depends on the interplay between multiple phytohormones. However, besides ethylene, the impact of other hormones on fruit quality traits remains elusive. A previous study has shown that down-regulation of SlARF4, a member of the tomato (Solanum lycopersicum) auxin response factor (ARF) gene family, results in a dark-green fruit phenotype with increased chloroplasts (Jones et al., 2002). This study further examines the role of this auxin transcriptional regulator during tomato fruit development at the level of transcripts, enzyme activities, and metabolites. It is noteworthy that the dark-green phenotype of antisense SlARF4-suppressed lines is restricted to fruit, suggesting that SlARF4 controls chlorophyll accumulation specifically in this organ. The SlARF4 underexpressing lines accumulate more starch at early stages of fruit development and display enhanced chlorophyll content and photochemical efficiency, which is consistent with the idea that fruit photosynthetic activity accounts for the elevated starch levels. SlARF4 expression is high in pericarp tissues of immature fruit and then undergoes a dramatic decline at the onset of ripening concomitant with the increase in sugar content. The higher starch content in developing fruits of SlARF4 down-regulated lines correlates with the up-regulation of genes and enzyme activities involved in starch biosynthesis, suggesting their negative regulation by SlARF4. Altogether, the data uncover the involvement of ARFs in the control of sugar content, an essential feature of fruit quality, and provide insight into the link between auxin signaling, chloroplastic activity, and sugar metabolism in developing fruit. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/23341361/SlARF4_an_auxin_response_factor_involved_in_the_control_of_sugar_metabolism_during_tomato_fruit_development_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=23341361 DB - PRIME DP - Unbound Medicine ER -