Tags

Type your tag names separated by a space and hit enter

Pick one, but be quick: 5' splice sites and the problems of too many choices.
Genes Dev. 2013 Jan 15; 27(2):129-44.GD

Abstract

Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5' Splice sites (5'ss) are the critical elements at the 5' end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5'ss in the human transcriptome. Most 5'ss are recognized by base-pairing with the 5' end of the U1 small nuclear RNA (snRNA). Here we review the history of research on 5'ss selection, highlighting the difficulties of establishing how base-pairing strength determines splicing outcomes. We also discuss recent work demonstrating that U1 snRNA:5'ss helices can accommodate noncanonical registers such as bulged duplexes. In addition, we describe the mechanisms by which other snRNAs, regulatory proteins, splicing enhancers, and the relative positions of alternative 5'ss contribute to selection. Moreover, we discuss mechanisms by which the recognition of numerous candidate 5'ss might lead to selection of a single 5'ss and propose that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5'ss selection.

Authors+Show Affiliations

School of Biological Sciences, Division of Molecular Genetics and Cell Biology, Nanyang Technological University, Singapore. xroca@ntu.edu.sgNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

23348838

Citation

Roca, Xavier, et al. "Pick One, but Be Quick: 5' Splice Sites and the Problems of Too Many Choices." Genes & Development, vol. 27, no. 2, 2013, pp. 129-44.
Roca X, Krainer AR, Eperon IC. Pick one, but be quick: 5' splice sites and the problems of too many choices. Genes Dev. 2013;27(2):129-44.
Roca, X., Krainer, A. R., & Eperon, I. C. (2013). Pick one, but be quick: 5' splice sites and the problems of too many choices. Genes & Development, 27(2), 129-44. https://doi.org/10.1101/gad.209759.112
Roca X, Krainer AR, Eperon IC. Pick One, but Be Quick: 5' Splice Sites and the Problems of Too Many Choices. Genes Dev. 2013 Jan 15;27(2):129-44. PubMed PMID: 23348838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pick one, but be quick: 5' splice sites and the problems of too many choices. AU - Roca,Xavier, AU - Krainer,Adrian R, AU - Eperon,Ian C, PY - 2013/1/26/entrez PY - 2013/1/26/pubmed PY - 2013/3/9/medline SP - 129 EP - 44 JF - Genes & development JO - Genes Dev. VL - 27 IS - 2 N2 - Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5' Splice sites (5'ss) are the critical elements at the 5' end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5'ss in the human transcriptome. Most 5'ss are recognized by base-pairing with the 5' end of the U1 small nuclear RNA (snRNA). Here we review the history of research on 5'ss selection, highlighting the difficulties of establishing how base-pairing strength determines splicing outcomes. We also discuss recent work demonstrating that U1 snRNA:5'ss helices can accommodate noncanonical registers such as bulged duplexes. In addition, we describe the mechanisms by which other snRNAs, regulatory proteins, splicing enhancers, and the relative positions of alternative 5'ss contribute to selection. Moreover, we discuss mechanisms by which the recognition of numerous candidate 5'ss might lead to selection of a single 5'ss and propose that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5'ss selection. SN - 1549-5477 UR - https://www.unboundmedicine.com/medline/citation/23348838/Pick_one_but_be_quick:_5'_splice_sites_and_the_problems_of_too_many_choices_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=23348838 DB - PRIME DP - Unbound Medicine ER -