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Inhibition of estradiol uptake and transforming growth factor alpha secretion in human breast cancer cell line MCF-7 by an alkyl-lysophospholipid.
Cancer Res. 1990 Jun 01; 50(11):3172-5.CR

Abstract

We investigated the effect of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3), an alkyl-lysophospholipid, on the uptake of estrogen, the secretion of transforming growth factor (TGF) alpha and the content of progesterone receptors (PRs) in the hormone-dependent breast cancer cell line, MCF-7. The uptake of labeled estradiol by MCF-7 was dose dependently decreased by 12 h pretreatment with 10-25 micrograms/ml ET-18-OCH3, and this suppression occurred prior to the onset of the inhibitory action of ET-18-OCH3 on MCF-7 growth. Scatchard analysis demonstrated that ET-18-OCH3 reduced the number of estrogen receptors in MCF-7 without affecting their affinity. Both the secretion of TGF-alpha from MCF-7 into the conditioned medium and the PR content of MCF-7 were decreased by 48 h treatment with 10 micrograms/ml ET-18-OCH3. The estradiol uptake, the TGF-alpha secretion, and the PR content were not affected by platelet-activating factor, lyso-PAF, and palmitoyl-lysophosphatidylcholine, all at 10 micrograms/ml. These results suggest that the reduction of estrogen receptor level induced by ET-18-OCH3 resulted in decreases in both the secretion of TGF-alpha and the content of PR in MCF-7, and these effects are specific to ET-18-OCH3. We concluded that these effects of ET-18-OCH3 may lead, at least partly, to its antitumor action in hormone-dependent breast cancer cell lines.

Authors+Show Affiliations

Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2334912

Citation

Kosano, H, et al. "Inhibition of Estradiol Uptake and Transforming Growth Factor Alpha Secretion in Human Breast Cancer Cell Line MCF-7 By an Alkyl-lysophospholipid." Cancer Research, vol. 50, no. 11, 1990, pp. 3172-5.
Kosano H, Yasutomo Y, Kugai N, et al. Inhibition of estradiol uptake and transforming growth factor alpha secretion in human breast cancer cell line MCF-7 by an alkyl-lysophospholipid. Cancer Res. 1990;50(11):3172-5.
Kosano, H., Yasutomo, Y., Kugai, N., Nagata, N., Inagaki, H., Tanaka, S., & Takatani, O. (1990). Inhibition of estradiol uptake and transforming growth factor alpha secretion in human breast cancer cell line MCF-7 by an alkyl-lysophospholipid. Cancer Research, 50(11), 3172-5.
Kosano H, et al. Inhibition of Estradiol Uptake and Transforming Growth Factor Alpha Secretion in Human Breast Cancer Cell Line MCF-7 By an Alkyl-lysophospholipid. Cancer Res. 1990 Jun 1;50(11):3172-5. PubMed PMID: 2334912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of estradiol uptake and transforming growth factor alpha secretion in human breast cancer cell line MCF-7 by an alkyl-lysophospholipid. AU - Kosano,H, AU - Yasutomo,Y, AU - Kugai,N, AU - Nagata,N, AU - Inagaki,H, AU - Tanaka,S, AU - Takatani,O, PY - 1990/6/1/pubmed PY - 1990/6/1/medline PY - 1990/6/1/entrez SP - 3172 EP - 5 JF - Cancer research JO - Cancer Res VL - 50 IS - 11 N2 - We investigated the effect of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3), an alkyl-lysophospholipid, on the uptake of estrogen, the secretion of transforming growth factor (TGF) alpha and the content of progesterone receptors (PRs) in the hormone-dependent breast cancer cell line, MCF-7. The uptake of labeled estradiol by MCF-7 was dose dependently decreased by 12 h pretreatment with 10-25 micrograms/ml ET-18-OCH3, and this suppression occurred prior to the onset of the inhibitory action of ET-18-OCH3 on MCF-7 growth. Scatchard analysis demonstrated that ET-18-OCH3 reduced the number of estrogen receptors in MCF-7 without affecting their affinity. Both the secretion of TGF-alpha from MCF-7 into the conditioned medium and the PR content of MCF-7 were decreased by 48 h treatment with 10 micrograms/ml ET-18-OCH3. The estradiol uptake, the TGF-alpha secretion, and the PR content were not affected by platelet-activating factor, lyso-PAF, and palmitoyl-lysophosphatidylcholine, all at 10 micrograms/ml. These results suggest that the reduction of estrogen receptor level induced by ET-18-OCH3 resulted in decreases in both the secretion of TGF-alpha and the content of PR in MCF-7, and these effects are specific to ET-18-OCH3. We concluded that these effects of ET-18-OCH3 may lead, at least partly, to its antitumor action in hormone-dependent breast cancer cell lines. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/2334912/Inhibition_of_estradiol_uptake_and_transforming_growth_factor_alpha_secretion_in_human_breast_cancer_cell_line_MCF_7_by_an_alkyl_lysophospholipid_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=2334912 DB - PRIME DP - Unbound Medicine ER -