Tags

Type your tag names separated by a space and hit enter

The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis.
J Biol Chem. 2013 Mar 08; 288(10):7204-14.JB

Abstract

Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) are essential for the formation of dentin. Previous in vitro studies have indicated that DMP1 might regulate the expression of DSPP during dentinogenesis. To examine whether DMP1 controls dentinogenesis through the regulation of DSPP in vivo, we cross-bred transgenic mice expressing normal DSPP driven by a 3.6-kb rat Col1a1 promoter with Dmp1 KO mice to generate mice expressing the DSPP transgene in the Dmp1 KO genetic background (referred to as "Dmp1 KO/DSPP Tg mice"). We used morphological, histological, and biochemical techniques to characterize the dentin and alveolar bone of Dmp1 KO/DSPP Tg mice compared with Dmp1 KO and wild-type mice. Our analyses showed that the expression of endogenous DSPP was remarkably reduced in the Dmp1 KO mice. Furthermore, the transgenic expression of DSPP rescued the tooth and alveolar bone defects of the Dmp1 KO mice. In addition, our in vitro analyses showed that DMP1 and its 57-kDa C-terminal fragment significantly up-regulated the Dspp promoter activities in a mesenchymal cell line. In contrast, the expression of DMP1 was not altered in the Dspp KO mice. These results provide strong evidence that DSPP is a downstream effector molecule that mediates the roles of DMP1 in dentinogenesis.

Authors+Show Affiliations

Department of Biomedical Sciences, Texas A&M Health Science Center, Baylor College of Dentistry, Dallas, Texas 75246, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23349460

Citation

Gibson, Monica Prasad, et al. "The Rescue of Dentin Matrix Protein 1 (DMP1)-deficient Tooth Defects By the Transgenic Expression of Dentin Sialophosphoprotein (DSPP) Indicates That DSPP Is a Downstream Effector Molecule of DMP1 in Dentinogenesis." The Journal of Biological Chemistry, vol. 288, no. 10, 2013, pp. 7204-14.
Gibson MP, Zhu Q, Wang S, et al. The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis. J Biol Chem. 2013;288(10):7204-14.
Gibson, M. P., Zhu, Q., Wang, S., Liu, Q., Liu, Y., Wang, X., Yuan, B., Ruest, L. B., Feng, J. Q., D'Souza, R. N., Qin, C., & Lu, Y. (2013). The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis. The Journal of Biological Chemistry, 288(10), 7204-14. https://doi.org/10.1074/jbc.M112.445775
Gibson MP, et al. The Rescue of Dentin Matrix Protein 1 (DMP1)-deficient Tooth Defects By the Transgenic Expression of Dentin Sialophosphoprotein (DSPP) Indicates That DSPP Is a Downstream Effector Molecule of DMP1 in Dentinogenesis. J Biol Chem. 2013 Mar 8;288(10):7204-14. PubMed PMID: 23349460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis. AU - Gibson,Monica Prasad, AU - Zhu,Qinglin, AU - Wang,Suzhen, AU - Liu,Qilin, AU - Liu,Ying, AU - Wang,Xiaofang, AU - Yuan,Baozhi, AU - Ruest,L Bruno, AU - Feng,Jian Q, AU - D'Souza,Rena N, AU - Qin,Chunlin, AU - Lu,Yongbo, Y1 - 2013/01/24/ PY - 2013/1/26/entrez PY - 2013/1/26/pubmed PY - 2013/5/1/medline SP - 7204 EP - 14 JF - The Journal of biological chemistry JO - J Biol Chem VL - 288 IS - 10 N2 - Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) are essential for the formation of dentin. Previous in vitro studies have indicated that DMP1 might regulate the expression of DSPP during dentinogenesis. To examine whether DMP1 controls dentinogenesis through the regulation of DSPP in vivo, we cross-bred transgenic mice expressing normal DSPP driven by a 3.6-kb rat Col1a1 promoter with Dmp1 KO mice to generate mice expressing the DSPP transgene in the Dmp1 KO genetic background (referred to as "Dmp1 KO/DSPP Tg mice"). We used morphological, histological, and biochemical techniques to characterize the dentin and alveolar bone of Dmp1 KO/DSPP Tg mice compared with Dmp1 KO and wild-type mice. Our analyses showed that the expression of endogenous DSPP was remarkably reduced in the Dmp1 KO mice. Furthermore, the transgenic expression of DSPP rescued the tooth and alveolar bone defects of the Dmp1 KO mice. In addition, our in vitro analyses showed that DMP1 and its 57-kDa C-terminal fragment significantly up-regulated the Dspp promoter activities in a mesenchymal cell line. In contrast, the expression of DMP1 was not altered in the Dspp KO mice. These results provide strong evidence that DSPP is a downstream effector molecule that mediates the roles of DMP1 in dentinogenesis. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/23349460/The_rescue_of_dentin_matrix_protein_1__DMP1__deficient_tooth_defects_by_the_transgenic_expression_of_dentin_sialophosphoprotein__DSPP__indicates_that_DSPP_is_a_downstream_effector_molecule_of_DMP1_in_dentinogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(19)62397-5 DB - PRIME DP - Unbound Medicine ER -