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Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) larvae.
PLoS One. 2013; 8(1):e54270.Plos

Abstract

Ingestion of double stranded RNA (dsRNA) has been previously demonstrated to be effective in triggering RNA interference (RNAi) in western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte), providing potential novel opportunities for insect pest control. The putative Snf7 homolog of WCR (DvSnf7) has previously been shown to be an effective RNAi target for insect control, as DvSnf7 RNAi leads to lethality of WCR larvae. Snf7 functions as a part of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway which plays a crucial role in cellular housekeeping by internalization, transport, sorting and lysosomal degradation of transmembrane proteins. To understand the effects that lead to death of WCR larvae by DvSnf7 RNAi, we examined some of the distinct cellular processes associated with ESCRT functions such as de-ubiquitination of proteins and autophagy. Our data indicate that ubiquitinated proteins accumulate in DvSnf7 dsRNA-fed larval tissues and that the autophagy process seems to be impaired. These findings suggest that the malfunctioning of these cellular processes in both midgut and fat body tissues triggered by DvSnf7 RNAi were the main effects leading to the death of WCR. This study also illustrates that Snf7 is an essential gene in WCR and its functions are consistent with biological functions described for other eukaryotes.

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Authors+Show Affiliations

Ramaseshadri P
Department of Biotechnology, Monsanto Company, Chesterfield, Missouri, USA.
Segers G
No affiliation info available
Flannagan R
No affiliation info available
Wiggins E
No affiliation info available
Clinton W
No affiliation info available
Ilagan O
No affiliation info available
McNulty B
No affiliation info available
Clark T
No affiliation info available
Bolognesi R
No affiliation info available

MeSH

AnimalsApoptosisAutophagyCell Physiological PhenomenaColeopteraDigestive SystemEndosomal Sorting Complexes Required for TransportFat BodyGreen Fluorescent ProteinsImmunohistochemistryInsect ControlInsect ProteinsLarvaMicroscopy, ConfocalModels, GeneticRNA InterferenceRNA, Small InterferingReverse Transcriptase Polymerase Chain ReactionUbiquitinated ProteinsUbiquitinationZea mays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23349844

Citation

Ramaseshadri, Parthasarathy, et al. "Physiological and Cellular Responses Caused By RNAi- Mediated Suppression of Snf7 Orthologue in Western Corn Rootworm (Diabrotica Virgifera Virgifera) Larvae." PloS One, vol. 8, no. 1, 2013, pp. e54270.
Ramaseshadri P, Segers G, Flannagan R, et al. Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) larvae. PLoS One. 2013;8(1):e54270.
Ramaseshadri, P., Segers, G., Flannagan, R., Wiggins, E., Clinton, W., Ilagan, O., McNulty, B., Clark, T., & Bolognesi, R. (2013). Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) larvae. PloS One, 8(1), e54270. https://doi.org/10.1371/journal.pone.0054270
Ramaseshadri P, et al. Physiological and Cellular Responses Caused By RNAi- Mediated Suppression of Snf7 Orthologue in Western Corn Rootworm (Diabrotica Virgifera Virgifera) Larvae. PLoS One. 2013;8(1):e54270. PubMed PMID: 23349844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) larvae. AU - Ramaseshadri,Parthasarathy, AU - Segers,Gerrit, AU - Flannagan,Ronald, AU - Wiggins,Elizabeth, AU - Clinton,William, AU - Ilagan,Oliver, AU - McNulty,Brian, AU - Clark,Thomas, AU - Bolognesi,Renata, Y1 - 2013/01/18/ PY - 2012/08/16/received PY - 2012/12/11/accepted PY - 2013/1/26/entrez PY - 2013/1/26/pubmed PY - 2013/7/25/medline SP - e54270 EP - e54270 JF - PloS one JO - PLoS One VL - 8 IS - 1 N2 - Ingestion of double stranded RNA (dsRNA) has been previously demonstrated to be effective in triggering RNA interference (RNAi) in western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte), providing potential novel opportunities for insect pest control. The putative Snf7 homolog of WCR (DvSnf7) has previously been shown to be an effective RNAi target for insect control, as DvSnf7 RNAi leads to lethality of WCR larvae. Snf7 functions as a part of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway which plays a crucial role in cellular housekeeping by internalization, transport, sorting and lysosomal degradation of transmembrane proteins. To understand the effects that lead to death of WCR larvae by DvSnf7 RNAi, we examined some of the distinct cellular processes associated with ESCRT functions such as de-ubiquitination of proteins and autophagy. Our data indicate that ubiquitinated proteins accumulate in DvSnf7 dsRNA-fed larval tissues and that the autophagy process seems to be impaired. These findings suggest that the malfunctioning of these cellular processes in both midgut and fat body tissues triggered by DvSnf7 RNAi were the main effects leading to the death of WCR. This study also illustrates that Snf7 is an essential gene in WCR and its functions are consistent with biological functions described for other eukaryotes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23349844/Physiological_and_cellular_responses_caused_by_RNAi__mediated_suppression_of_Snf7_orthologue_in_western_corn_rootworm__Diabrotica_virgifera_virgifera__larvae_ L2 - https://dx.plos.org/10.1371/journal.pone.0054270 DB - PRIME DP - Unbound Medicine ER -