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Combined exposure of carps (Cyprinus carpio L.) to cyanobacterial biomass and white spot disease.
Neuro Endocrinol Lett. 2012; 33 Suppl 3:77-83.NE

Abstract

OBJECTIVES

Under environmental conditions, fish can be exposed to multiple stressors including natural toxins and infectious agents at the same time. This study brings new knowledge on the effects of controlled exposure to multiple stressors in fish. The aim of this study was to test the hypothesis that influence of cyanobacterial biomass and an infection agent represented by the white spot disease can combine to enhance the effects on fish.

METHODS

Common carps were divided into four groups, each with 40 specimens for 20 days: control group, cyanobacterial biomass exposed group, Ichthyophthirius multifiliis-infected fish (Ich) and cyanobacterial biomass-exposed fish + Ichthyophthirius multifiliis-infected fish. During the experiment we evaluated the clinical signs, mortality, selected haematological parameters, immune parameters and toxin accumulation.

RESULTS

There was no mortality in control fish and cyanobacterial biomass-exposed fish. One specimen died in Ichthyophthirius multifiliis-infected fish and the combined exposure resulted in the death of 13 specimens. The whole leukocyte counts (WBC) of the control group did not show any significant differences. Cyanobacteria alone caused a significant increase of the WBC on day 13 (p≤0.05) and on day 20 (p≤0.01). Also, I. multifiliis caused a significant elevation of WBC (p≤0.01) on day 20. Co-exposition resulted in WBC increased on day 13 and decrease on day 20, but the changes were not significant. It is evident from the differential leukocyte counts that while the increase of WBC in the group exposed to cyanobacteria was caused by elevation of lymphocytes, the increase in the group infected by I. multifiliis was due to the increase of myeloid cells. It well corresponds with the integral of chemiluminescence in the group infected by I. multifiliis, which is significantly elevated on day 20 in comparison with all other groups.

CONCLUSIONS

We can confirm additive action of different agents on the immune system of fish. While single agents seemed to stimulate the immune response, the combination of both caused immunosuppression.

Authors+Show Affiliations

Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. palikovam@vfu.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23353848

Citation

Palikova, Miroslava, et al. "Combined Exposure of Carps (Cyprinus Carpio L.) to Cyanobacterial Biomass and White Spot Disease." Neuro Endocrinology Letters, vol. 33 Suppl 3, 2012, pp. 77-83.
Palikova M, Navratil S, Papezikova I, et al. Combined exposure of carps (Cyprinus carpio L.) to cyanobacterial biomass and white spot disease. Neuro Endocrinol Lett. 2012;33 Suppl 3:77-83.
Palikova, M., Navratil, S., Papezikova, I., Ambroz, P., Vesely, T., Pokorova, D., Mares, J., Adamovsky, O., Navratil, L., & Kopp, R. (2012). Combined exposure of carps (Cyprinus carpio L.) to cyanobacterial biomass and white spot disease. Neuro Endocrinology Letters, 33 Suppl 3, 77-83.
Palikova M, et al. Combined Exposure of Carps (Cyprinus Carpio L.) to Cyanobacterial Biomass and White Spot Disease. Neuro Endocrinol Lett. 2012;33 Suppl 3:77-83. PubMed PMID: 23353848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined exposure of carps (Cyprinus carpio L.) to cyanobacterial biomass and white spot disease. AU - Palikova,Miroslava, AU - Navratil,Stanislav, AU - Papezikova,Ivana, AU - Ambroz,Petr, AU - Vesely,Tomas, AU - Pokorova,Dagmar, AU - Mares,Jan, AU - Adamovsky,Ondrej, AU - Navratil,Lukas, AU - Kopp,Radovan, PY - 2012/09/01/received PY - 2012/11/15/accepted PY - 2013/1/29/entrez PY - 2013/1/29/pubmed PY - 2013/4/12/medline SP - 77 EP - 83 JF - Neuro endocrinology letters JO - Neuro Endocrinol. Lett. VL - 33 Suppl 3 N2 - OBJECTIVES: Under environmental conditions, fish can be exposed to multiple stressors including natural toxins and infectious agents at the same time. This study brings new knowledge on the effects of controlled exposure to multiple stressors in fish. The aim of this study was to test the hypothesis that influence of cyanobacterial biomass and an infection agent represented by the white spot disease can combine to enhance the effects on fish. METHODS: Common carps were divided into four groups, each with 40 specimens for 20 days: control group, cyanobacterial biomass exposed group, Ichthyophthirius multifiliis-infected fish (Ich) and cyanobacterial biomass-exposed fish + Ichthyophthirius multifiliis-infected fish. During the experiment we evaluated the clinical signs, mortality, selected haematological parameters, immune parameters and toxin accumulation. RESULTS: There was no mortality in control fish and cyanobacterial biomass-exposed fish. One specimen died in Ichthyophthirius multifiliis-infected fish and the combined exposure resulted in the death of 13 specimens. The whole leukocyte counts (WBC) of the control group did not show any significant differences. Cyanobacteria alone caused a significant increase of the WBC on day 13 (p≤0.05) and on day 20 (p≤0.01). Also, I. multifiliis caused a significant elevation of WBC (p≤0.01) on day 20. Co-exposition resulted in WBC increased on day 13 and decrease on day 20, but the changes were not significant. It is evident from the differential leukocyte counts that while the increase of WBC in the group exposed to cyanobacteria was caused by elevation of lymphocytes, the increase in the group infected by I. multifiliis was due to the increase of myeloid cells. It well corresponds with the integral of chemiluminescence in the group infected by I. multifiliis, which is significantly elevated on day 20 in comparison with all other groups. CONCLUSIONS: We can confirm additive action of different agents on the immune system of fish. While single agents seemed to stimulate the immune response, the combination of both caused immunosuppression. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/23353848/Combined_exposure_of_carps__Cyprinus_carpio_L___to_cyanobacterial_biomass_and_white_spot_disease_ DB - PRIME DP - Unbound Medicine ER -