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Risk of combined exposure of birds to cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant.
Neuro Endocrinol Lett. 2012; 33 Suppl 3:155-60.NE

Abstract

OBJECTIVES

The objective of this study was to examine the hypothesis that a combination of cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant can enhance avian toxic effects produced by single exposures only.

METHODS

A total of 48 two-month-old Japanese quails (Coturnix coturnix japonica) with average body weight of 160 g were randomly divided into 8 experimental groups of six birds and sex ratio of 1:1. Experimental groups of control Japanese quails (C) and birds exposed to single and combined sub-lethal doses of paraoxon (P), bromadiolone (B), and microcystins in cyanobacterial biomass (M) included: C, P, P+B, B, B+M, P+M, M, and P+B+M. During the 10-day exposure birds in the respective groups received biomass containing 61.62 µg microcystins daily (i.e. 26.54 µg MC-RR, 7.62 µg MC-YR and 27.39 µg MC-LR), two 250 μg/kg doses of paraoxon, and two 500 mg/kg doses of bromadiolone. Group responses were compared using standard plasma biochemistry and antioxidant/oxidative stress parameters in tissues.

RESULTS

While single and double combinations of toxicants induced responses in individual biochemical parameters measured and evaluated using univariate statistical analysis, those in the triple exposure were most extensive. The principal component analysis of antioxidant/oxidative stress parameters (glutathione reductase, lipid peroxidation, and ferric reducing antioxidant power) in tissues (liver, kidney, heart, brain, lungs, gonads, and pectoralis major muscle) clearly separated the triple group (P+B+M) from all single and double exposure groups and the control and indicated thus marked joint effects in the overall pattern of antioxidant/oxidative stress responses of this group. The separation was driven by the modification of the ferric reducing antioxidant power levels in heart and brain and the cardiac lipid peroxidation level, in particular.

CONCLUSIONS

This experiment contributes to the understanding of the pathogenic mechanisms of combined sub-lethal exposure to natural toxins and agrochemicals and may be used for risk assessment of environmental pollution in birds.

Authors+Show Affiliations

Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23353860

Citation

Ondracek, Karel, et al. "Risk of Combined Exposure of Birds to Cyanobacterial Biomass Containing Microcystins, Acetylcholinesterase Inhibitor and Anticoagulant." Neuro Endocrinology Letters, vol. 33 Suppl 3, 2012, pp. 155-60.
Ondracek K, Bandouchova H, Damkova V, et al. Risk of combined exposure of birds to cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant. Neuro Endocrinol Lett. 2012;33 Suppl 3:155-60.
Ondracek, K., Bandouchova, H., Damkova, V., Hilscherova, K., Kral, J., Osickova, J., Mlcakova, V., Pohanka, M., Skochova, H., Vitula, F., Treml, F., & Pikula, J. (2012). Risk of combined exposure of birds to cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant. Neuro Endocrinology Letters, 33 Suppl 3, 155-60.
Ondracek K, et al. Risk of Combined Exposure of Birds to Cyanobacterial Biomass Containing Microcystins, Acetylcholinesterase Inhibitor and Anticoagulant. Neuro Endocrinol Lett. 2012;33 Suppl 3:155-60. PubMed PMID: 23353860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Risk of combined exposure of birds to cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant. AU - Ondracek,Karel, AU - Bandouchova,Hana, AU - Damkova,Veronika, AU - Hilscherova,Klara, AU - Kral,Jiri, AU - Osickova,Jitka, AU - Mlcakova,Veronika, AU - Pohanka,Miroslav, AU - Skochova,Hana, AU - Vitula,Frantisek, AU - Treml,Frantisek, AU - Pikula,Jiri, PY - 2012/09/01/received PY - 2012/11/15/accepted PY - 2013/1/29/entrez PY - 2013/1/29/pubmed PY - 2013/4/12/medline SP - 155 EP - 60 JF - Neuro endocrinology letters JO - Neuro Endocrinol. Lett. VL - 33 Suppl 3 N2 - OBJECTIVES: The objective of this study was to examine the hypothesis that a combination of cyanobacterial biomass containing microcystins, acetylcholinesterase inhibitor and anticoagulant can enhance avian toxic effects produced by single exposures only. METHODS: A total of 48 two-month-old Japanese quails (Coturnix coturnix japonica) with average body weight of 160 g were randomly divided into 8 experimental groups of six birds and sex ratio of 1:1. Experimental groups of control Japanese quails (C) and birds exposed to single and combined sub-lethal doses of paraoxon (P), bromadiolone (B), and microcystins in cyanobacterial biomass (M) included: C, P, P+B, B, B+M, P+M, M, and P+B+M. During the 10-day exposure birds in the respective groups received biomass containing 61.62 µg microcystins daily (i.e. 26.54 µg MC-RR, 7.62 µg MC-YR and 27.39 µg MC-LR), two 250 μg/kg doses of paraoxon, and two 500 mg/kg doses of bromadiolone. Group responses were compared using standard plasma biochemistry and antioxidant/oxidative stress parameters in tissues. RESULTS: While single and double combinations of toxicants induced responses in individual biochemical parameters measured and evaluated using univariate statistical analysis, those in the triple exposure were most extensive. The principal component analysis of antioxidant/oxidative stress parameters (glutathione reductase, lipid peroxidation, and ferric reducing antioxidant power) in tissues (liver, kidney, heart, brain, lungs, gonads, and pectoralis major muscle) clearly separated the triple group (P+B+M) from all single and double exposure groups and the control and indicated thus marked joint effects in the overall pattern of antioxidant/oxidative stress responses of this group. The separation was driven by the modification of the ferric reducing antioxidant power levels in heart and brain and the cardiac lipid peroxidation level, in particular. CONCLUSIONS: This experiment contributes to the understanding of the pathogenic mechanisms of combined sub-lethal exposure to natural toxins and agrochemicals and may be used for risk assessment of environmental pollution in birds. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/23353860/Risk_of_combined_exposure_of_birds_to_cyanobacterial_biomass_containing_microcystins_acetylcholinesterase_inhibitor_and_anticoagulant_ DB - PRIME DP - Unbound Medicine ER -