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Role of the pentose phosphate pathway and the Entner-Doudoroff pathway in glucose metabolism of Gluconobacter oxydans 621H.
Appl Microbiol Biotechnol. 2013 May; 97(10):4315-23.AM

Abstract

Glucose catabolism by the obligatory aerobic acetic acid bacterium Gluconobacter oxydans 621H proceeds in two phases comprising rapid periplasmic oxidation of glucose to gluconate (phase I) and oxidation of gluconate to 2-ketogluconate or 5-ketogluconate (phase II). Only a small amount of glucose and part of the gluconate is taken up into the cells. To determine the roles of the pentose phosphate pathway (PPP) and the Entner-Doudoroff pathway (EDP) for intracellular glucose and gluconate catabolism, mutants defective in either the PPP (Δgnd, Δgnd zwf*) or the EDP (Δedd-eda) were characterized under defined conditions of pH 6 and 15 % dissolved oxygen. In the presence of yeast extract, neither of the two pathways was essential for growth with glucose. However, the PPP mutants showed a reduced growth rate in phase I and completely lacked growth in phase II. In contrast, the EDP mutant showed the same growth behavior as the reference strain. These results demonstrate that the PPP is of major importance for cytoplasmic glucose and gluconate catabolism, whereas the EDP is dispensable. Reasons for this difference are discussed.

Authors+Show Affiliations

Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, Jülich 52425, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23354449

Citation

Richhardt, Janine, et al. "Role of the Pentose Phosphate Pathway and the Entner-Doudoroff Pathway in Glucose Metabolism of Gluconobacter Oxydans 621H." Applied Microbiology and Biotechnology, vol. 97, no. 10, 2013, pp. 4315-23.
Richhardt J, Bringer S, Bott M. Role of the pentose phosphate pathway and the Entner-Doudoroff pathway in glucose metabolism of Gluconobacter oxydans 621H. Appl Microbiol Biotechnol. 2013;97(10):4315-23.
Richhardt, J., Bringer, S., & Bott, M. (2013). Role of the pentose phosphate pathway and the Entner-Doudoroff pathway in glucose metabolism of Gluconobacter oxydans 621H. Applied Microbiology and Biotechnology, 97(10), 4315-23. https://doi.org/10.1007/s00253-013-4707-2
Richhardt J, Bringer S, Bott M. Role of the Pentose Phosphate Pathway and the Entner-Doudoroff Pathway in Glucose Metabolism of Gluconobacter Oxydans 621H. Appl Microbiol Biotechnol. 2013;97(10):4315-23. PubMed PMID: 23354449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the pentose phosphate pathway and the Entner-Doudoroff pathway in glucose metabolism of Gluconobacter oxydans 621H. AU - Richhardt,Janine, AU - Bringer,Stephanie, AU - Bott,Michael, Y1 - 2013/01/25/ PY - 2012/11/13/received PY - 2013/01/10/accepted PY - 2013/01/09/revised PY - 2013/1/29/entrez PY - 2013/1/29/pubmed PY - 2013/10/22/medline SP - 4315 EP - 23 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 97 IS - 10 N2 - Glucose catabolism by the obligatory aerobic acetic acid bacterium Gluconobacter oxydans 621H proceeds in two phases comprising rapid periplasmic oxidation of glucose to gluconate (phase I) and oxidation of gluconate to 2-ketogluconate or 5-ketogluconate (phase II). Only a small amount of glucose and part of the gluconate is taken up into the cells. To determine the roles of the pentose phosphate pathway (PPP) and the Entner-Doudoroff pathway (EDP) for intracellular glucose and gluconate catabolism, mutants defective in either the PPP (Δgnd, Δgnd zwf*) or the EDP (Δedd-eda) were characterized under defined conditions of pH 6 and 15 % dissolved oxygen. In the presence of yeast extract, neither of the two pathways was essential for growth with glucose. However, the PPP mutants showed a reduced growth rate in phase I and completely lacked growth in phase II. In contrast, the EDP mutant showed the same growth behavior as the reference strain. These results demonstrate that the PPP is of major importance for cytoplasmic glucose and gluconate catabolism, whereas the EDP is dispensable. Reasons for this difference are discussed. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/23354449/Role_of_the_pentose_phosphate_pathway_and_the_Entner_Doudoroff_pathway_in_glucose_metabolism_of_Gluconobacter_oxydans_621H_ L2 - https://dx.doi.org/10.1007/s00253-013-4707-2 DB - PRIME DP - Unbound Medicine ER -