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Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis.
BMC Complement Altern Med. 2013 Jan 28; 13:23.BC

Abstract

BACKGROUND

Geopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. However, studies concerning the biological activity and chemical composition of geopropolis are scarce. In this study, we evaluated the antimicrobial and antiproliferative activity of the ethanolic extract of geopropolis (EEGP) collected by Melipona scutellaris and its bioactive fraction against important clinical microorganisms as well as their in vitro cytotoxicity and chemical profile.

METHODS

The antimicrobial activity of EEGP and fractions was examined by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against six bacteria strains as well as their ability to inhibit Streptococcus mutans biofilm adherence. Total growth inhibition (TGI) was chosen to assay the antiproliferative activity of EEGP and its bioactive fraction against normal and cancer cell lines. The chemical composition of M. scutellaris geopropolis was identified by reversed-phase high-performance liquid chromatography and gas chromatography-mass spectrometry.

RESULTS

EEGP significantly inhibited the growth of Staphylococcus aureus strains and S. mutans at low concentrations, and its hexane fraction (HF) presented the highest antibacterial activity. Also, both EEGP and HF inhibited S. mutans biofilm adherence (p < 0.05) and showed selectivity against human cancer cell lines, although only HF demonstrated selectivity at low concentrations. The chemical analyses performed suggest the absence of flavonoids and the presence of benzophenones as geopropolis major compounds.

CONCLUSIONS

The empirical use of this unique type of geopropolis by folk medicine practitioners was confirmed in the present study, since it showed antimicrobial and antiproliferative potential against the cancer cell lines studied. It is possible that the major compounds found in this type of geopropolis are responsible for its properties.

Authors+Show Affiliations

Department of Physiological Sciences, Piracicaba Dental School, University of Campinas UNICAMP, Av, Limeira 901, 13414-903 Piracicaba, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23356696

Citation

da Cunha, Marcos Guilherme, et al. "Antimicrobial and Antiproliferative Activities of Stingless Bee Melipona Scutellaris Geopropolis." BMC Complementary and Alternative Medicine, vol. 13, 2013, p. 23.
da Cunha MG, Franchin M, de Carvalho Galvão LC, et al. Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis. BMC Complement Altern Med. 2013;13:23.
da Cunha, M. G., Franchin, M., de Carvalho Galvão, L. C., de Ruiz, A. L., de Carvalho, J. E., Ikegaki, M., de Alencar, S. M., Koo, H., & Rosalen, P. L. (2013). Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis. BMC Complementary and Alternative Medicine, 13, 23. https://doi.org/10.1186/1472-6882-13-23
da Cunha MG, et al. Antimicrobial and Antiproliferative Activities of Stingless Bee Melipona Scutellaris Geopropolis. BMC Complement Altern Med. 2013 Jan 28;13:23. PubMed PMID: 23356696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis. AU - da Cunha,Marcos Guilherme, AU - Franchin,Marcelo, AU - de Carvalho Galvão,Lívia Câmara, AU - de Ruiz,Ana Lúcia Tasca Góis, AU - de Carvalho,João Ernesto, AU - Ikegaki,Masarahu, AU - de Alencar,Severino Matias, AU - Koo,Hyun, AU - Rosalen,Pedro Luiz, Y1 - 2013/01/28/ PY - 2012/08/29/received PY - 2013/01/21/accepted PY - 2013/1/30/entrez PY - 2013/1/30/pubmed PY - 2013/6/19/medline SP - 23 EP - 23 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 13 N2 - BACKGROUND: Geopropolis is a type of propolis containing resin, wax, and soil, collected by threatened stingless bee species native to tropical countries and used in folk medicine. However, studies concerning the biological activity and chemical composition of geopropolis are scarce. In this study, we evaluated the antimicrobial and antiproliferative activity of the ethanolic extract of geopropolis (EEGP) collected by Melipona scutellaris and its bioactive fraction against important clinical microorganisms as well as their in vitro cytotoxicity and chemical profile. METHODS: The antimicrobial activity of EEGP and fractions was examined by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against six bacteria strains as well as their ability to inhibit Streptococcus mutans biofilm adherence. Total growth inhibition (TGI) was chosen to assay the antiproliferative activity of EEGP and its bioactive fraction against normal and cancer cell lines. The chemical composition of M. scutellaris geopropolis was identified by reversed-phase high-performance liquid chromatography and gas chromatography-mass spectrometry. RESULTS: EEGP significantly inhibited the growth of Staphylococcus aureus strains and S. mutans at low concentrations, and its hexane fraction (HF) presented the highest antibacterial activity. Also, both EEGP and HF inhibited S. mutans biofilm adherence (p < 0.05) and showed selectivity against human cancer cell lines, although only HF demonstrated selectivity at low concentrations. The chemical analyses performed suggest the absence of flavonoids and the presence of benzophenones as geopropolis major compounds. CONCLUSIONS: The empirical use of this unique type of geopropolis by folk medicine practitioners was confirmed in the present study, since it showed antimicrobial and antiproliferative potential against the cancer cell lines studied. It is possible that the major compounds found in this type of geopropolis are responsible for its properties. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/23356696/Antimicrobial_and_antiproliferative_activities_of_stingless_bee_Melipona_scutellaris_geopropolis_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-23 DB - PRIME DP - Unbound Medicine ER -