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The relation between adult dark spermatogonia and other parameters of fertility potential in cryptorchid testes.
J Urol. 2013 Oct; 190(4 Suppl):1566-71.JU

Abstract

PURPOSE

The fertility potential of boys with cryptorchidism may be related to the number of adult dark spermatogonia per tubular transverse section in testicular biopsies taken at orchiopexy. Placental-like alkaline phosphatase positive gonocytes in testes within year 1 of life indicate preserved ability for germ cell transformation. We related these parameters to the total number of tubular germ cells and other factors associated with fertility potential.

MATERIALS AND METHODS

The study comprised 89 boys 0.7 to 3 years old (median age 1.8) who underwent bilateral testicular biopsy at bilateral orchiopexy and provided blood samples for gonadotropins and inhibin B.

RESULTS

Of 76 boys with adult dark spermatogonia 44 (58%) had a normal mean number of spermatogonia per tubular transverse section compared to 2 of 13 (15%) without adult dark spermatogonia (p <0.05). In the 30 boys with good fertility potential, including a normal mean number of tubular germ cells, and normal gonadotropins and inhibin B, the mean number of adult dark tubular germ cells was 0.081 vs 0.031 in the 38 with low fertility potential, including impaired tubular germ cells and/or low inhibin B but no reactive increase in gonadotropins (p <0.05). In the 21 patients with increased gonadotropins the mean number of adult dark spermatogonia per tubular transverse section was 0.063. Of the 20 boys with normal mean adult dark spermatogonia per tubular transverse section 12 (60%) had good fertility potential, including a normal mean number of tubular germ cells, normal gonadotropins and normal inhibin B, compared to only 18 of 69 (26%) with an impaired mean number of adult dark spermatogonia per tubular transverse section (p <0.05). Of 46 boys with a normal mean number of tubular germ cells 26 (57%) had placental-like alkaline phosphatase positive cells compared to 14 of 43 (33%) with a decreased mean number of tubular germ cells (p <0.05).

CONCLUSIONS

The number of placental-like alkaline phosphatase positive gonocytes and adult dark spermatogonia per tubular transverse section are important parameters related to the fertility potential of boys with cryptorchid testes.

Authors+Show Affiliations

Department of Pediatric Surgery, Rigshospitalet, Copenhagen, Denmark; Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23357211

Citation

Thorup, Jorgen, et al. "The Relation Between Adult Dark Spermatogonia and Other Parameters of Fertility Potential in Cryptorchid Testes." The Journal of Urology, vol. 190, no. 4 Suppl, 2013, pp. 1566-71.
Thorup J, Kvist K, Clasen-Linde E, et al. The relation between adult dark spermatogonia and other parameters of fertility potential in cryptorchid testes. J Urol. 2013;190(4 Suppl):1566-71.
Thorup, J., Kvist, K., Clasen-Linde, E., Petersen, B. L., & Cortes, D. (2013). The relation between adult dark spermatogonia and other parameters of fertility potential in cryptorchid testes. The Journal of Urology, 190(4 Suppl), 1566-71. https://doi.org/10.1016/j.juro.2013.01.058
Thorup J, et al. The Relation Between Adult Dark Spermatogonia and Other Parameters of Fertility Potential in Cryptorchid Testes. J Urol. 2013;190(4 Suppl):1566-71. PubMed PMID: 23357211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The relation between adult dark spermatogonia and other parameters of fertility potential in cryptorchid testes. AU - Thorup,Jorgen, AU - Kvist,Kolja, AU - Clasen-Linde,Erik, AU - Petersen,Bodil Laub, AU - Cortes,Dina, Y1 - 2013/01/25/ PY - 2013/01/17/accepted PY - 2013/1/30/entrez PY - 2013/1/30/pubmed PY - 2013/11/15/medline KW - Ad KW - Ad spermatogonia per transverse tubule KW - Ad/T KW - FSH KW - LH KW - PLAP KW - S/T KW - adult dark KW - cryptorchidism KW - follicle-stimulating hormone KW - germ cells per transverse tubule KW - gonadotropins KW - infertility, male KW - inhibin B KW - luteinizing hormone KW - placental-like alkaline phosphatase KW - testis SP - 1566 EP - 71 JF - The Journal of urology JO - J. Urol. VL - 190 IS - 4 Suppl N2 - PURPOSE: The fertility potential of boys with cryptorchidism may be related to the number of adult dark spermatogonia per tubular transverse section in testicular biopsies taken at orchiopexy. Placental-like alkaline phosphatase positive gonocytes in testes within year 1 of life indicate preserved ability for germ cell transformation. We related these parameters to the total number of tubular germ cells and other factors associated with fertility potential. MATERIALS AND METHODS: The study comprised 89 boys 0.7 to 3 years old (median age 1.8) who underwent bilateral testicular biopsy at bilateral orchiopexy and provided blood samples for gonadotropins and inhibin B. RESULTS: Of 76 boys with adult dark spermatogonia 44 (58%) had a normal mean number of spermatogonia per tubular transverse section compared to 2 of 13 (15%) without adult dark spermatogonia (p <0.05). In the 30 boys with good fertility potential, including a normal mean number of tubular germ cells, and normal gonadotropins and inhibin B, the mean number of adult dark tubular germ cells was 0.081 vs 0.031 in the 38 with low fertility potential, including impaired tubular germ cells and/or low inhibin B but no reactive increase in gonadotropins (p <0.05). In the 21 patients with increased gonadotropins the mean number of adult dark spermatogonia per tubular transverse section was 0.063. Of the 20 boys with normal mean adult dark spermatogonia per tubular transverse section 12 (60%) had good fertility potential, including a normal mean number of tubular germ cells, normal gonadotropins and normal inhibin B, compared to only 18 of 69 (26%) with an impaired mean number of adult dark spermatogonia per tubular transverse section (p <0.05). Of 46 boys with a normal mean number of tubular germ cells 26 (57%) had placental-like alkaline phosphatase positive cells compared to 14 of 43 (33%) with a decreased mean number of tubular germ cells (p <0.05). CONCLUSIONS: The number of placental-like alkaline phosphatase positive gonocytes and adult dark spermatogonia per tubular transverse section are important parameters related to the fertility potential of boys with cryptorchid testes. SN - 1527-3792 UR - https://www.unboundmedicine.com/medline/citation/23357211/The_relation_between_adult_dark_spermatogonia_and_other_parameters_of_fertility_potential_in_cryptorchid_testes_ L2 - https://www.jurology.com/doi/full/10.1016/j.juro.2013.01.058?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -