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Aronia melanocarpa as a protector against nitration of fibrinogen.
Int J Biol Macromol. 2013 Apr; 55:264-8.IJ

Abstract

Fibrinogen (Fg) also known as coagulation factor I represents about 4% of the total human plasma proteins. The main function of Fg is its involvement in last phase of blood coagulation cascade, when thrombin-induced conversion of dissolved plasma fibrinogen into an insoluble fibrin clot occurs. The reaction of fibrinogen with peroxynitrite causes both structural modifications and changes of the biological properties of this plasma glycoprotein. Recently, there is an increased interest in the screening of natural products present in fruits, vegetables and herbs for their possible antioxidative activities. Therefore, the aim of our study was to estimate the effect of extract from berries of Aronia melanocarpa against nitrative and oxidative damage induced by peroxynitrite. The extract from A. melanocarpa (0.5-50 μg/ml) added to Fg 10 min before peroxynitrite (100 μM) significantly inhibited both the formation of the high molecular weight protein aggregates and nitration of Fg molecule. The extract also abolished peroxynitrite-induced inhibition of fibrinogen polymerization (by 95% at 50 μg/ml). The obtained results indicate that natural extract from berries of A. melanocarpa has protective effects against peroxynitrite-induced nitrative damage of plasma fibrinogen, and therefore may contribute in the prevention of peroxynitrite-related cardiovascular or inflammatory diseases.

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Authors+Show Affiliations

Bijak M
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. mbijak@biol.uni.lodz.pl
Saluk J
No affiliation info available
Antosik A
No affiliation info available
Ponczek MB
No affiliation info available
Żbikowska HM
No affiliation info available
Borowiecka M
No affiliation info available
Nowak P
No affiliation info available

MeSH

Dose-Response Relationship, DrugFibrinogenHumansOxidation-ReductionPeroxynitrous AcidPhotiniaPlant ExtractsProtein Multimerization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23357800

Citation

Bijak, Michał, et al. "Aronia Melanocarpa as a Protector Against Nitration of Fibrinogen." International Journal of Biological Macromolecules, vol. 55, 2013, pp. 264-8.
Bijak M, Saluk J, Antosik A, et al. Aronia melanocarpa as a protector against nitration of fibrinogen. Int J Biol Macromol. 2013;55:264-8.
Bijak, M., Saluk, J., Antosik, A., Ponczek, M. B., Żbikowska, H. M., Borowiecka, M., & Nowak, P. (2013). Aronia melanocarpa as a protector against nitration of fibrinogen. International Journal of Biological Macromolecules, 55, 264-8. https://doi.org/10.1016/j.ijbiomac.2013.01.019
Bijak M, et al. Aronia Melanocarpa as a Protector Against Nitration of Fibrinogen. Int J Biol Macromol. 2013;55:264-8. PubMed PMID: 23357800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aronia melanocarpa as a protector against nitration of fibrinogen. AU - Bijak,Michał, AU - Saluk,Joanna, AU - Antosik,Adam, AU - Ponczek,Michał B, AU - Żbikowska,Halina M, AU - Borowiecka,Marta, AU - Nowak,Paweł, Y1 - 2013/01/25/ PY - 2012/11/18/received PY - 2012/12/18/revised PY - 2013/01/13/accepted PY - 2013/1/30/entrez PY - 2013/1/30/pubmed PY - 2013/8/24/medline SP - 264 EP - 8 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 55 N2 - Fibrinogen (Fg) also known as coagulation factor I represents about 4% of the total human plasma proteins. The main function of Fg is its involvement in last phase of blood coagulation cascade, when thrombin-induced conversion of dissolved plasma fibrinogen into an insoluble fibrin clot occurs. The reaction of fibrinogen with peroxynitrite causes both structural modifications and changes of the biological properties of this plasma glycoprotein. Recently, there is an increased interest in the screening of natural products present in fruits, vegetables and herbs for their possible antioxidative activities. Therefore, the aim of our study was to estimate the effect of extract from berries of Aronia melanocarpa against nitrative and oxidative damage induced by peroxynitrite. The extract from A. melanocarpa (0.5-50 μg/ml) added to Fg 10 min before peroxynitrite (100 μM) significantly inhibited both the formation of the high molecular weight protein aggregates and nitration of Fg molecule. The extract also abolished peroxynitrite-induced inhibition of fibrinogen polymerization (by 95% at 50 μg/ml). The obtained results indicate that natural extract from berries of A. melanocarpa has protective effects against peroxynitrite-induced nitrative damage of plasma fibrinogen, and therefore may contribute in the prevention of peroxynitrite-related cardiovascular or inflammatory diseases. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/23357800/Aronia_melanocarpa_as_a_protector_against_nitration_of_fibrinogen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(13)00026-3 DB - PRIME DP - Unbound Medicine ER -