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Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways.
J Agric Food Chem. 2013 Feb 20; 61(7):1509-20.JA

Abstract

Chicoric acid has been reported to possess various bioactivities. However, the antiobesity effects of chicoric acid remain poorly understood. In this study, we investigated the effects of chicoric acid on 3T3-L1 preadipocytes and its molecular mechanisms of apoptosis. Chicoric acid inhibited cell viability and induced apoptosis in 3T3-L1 preadipocytes which was characterized by chromatin condensation and poly ADP-ribose-polymerase (PARP) cleavage. Mitochondrial membrane potential (MMP) loss, Bax/Bcl-2 dysregulation, cytochrome c release, and caspase-3 activation were observed, indicating mitochondria-dependent apoptosis induced by chicoric acid. Furthermore, PI3K/Akt and MAPK (p38 MAPK, JNK, and ERK1/2) signaling pathways were involved in chicoric acid-induced apoptosis. The employment of protein kinase inhibitors LY294002, SB203580, SP600125, and U0126 revealed that PI3K/Akt signaling pathway interplayed with MAPK signaling pathways. Moreover, chicoric acid induced reactive oxygen species (ROS) generation. Pretreatment with the antioxidant N-acetylcysteine (NAC) significantly blocked cell death and changes of Akt and MAPK signalings induced by chicoric acid. In addition, chicoric acid down regulated HO-1 and COX-2 via the PI3K/Akt pathway.

Authors+Show Affiliations

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23363008

Citation

Xiao, Haifang, et al. "Chicoric Acid Induces Apoptosis in 3T3-L1 Preadipocytes Through ROS-mediated PI3K/Akt and MAPK Signaling Pathways." Journal of Agricultural and Food Chemistry, vol. 61, no. 7, 2013, pp. 1509-20.
Xiao H, Wang J, Yuan L, et al. Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. J Agric Food Chem. 2013;61(7):1509-20.
Xiao, H., Wang, J., Yuan, L., Xiao, C., Wang, Y., & Liu, X. (2013). Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. Journal of Agricultural and Food Chemistry, 61(7), 1509-20. https://doi.org/10.1021/jf3050268
Xiao H, et al. Chicoric Acid Induces Apoptosis in 3T3-L1 Preadipocytes Through ROS-mediated PI3K/Akt and MAPK Signaling Pathways. J Agric Food Chem. 2013 Feb 20;61(7):1509-20. PubMed PMID: 23363008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. AU - Xiao,Haifang, AU - Wang,Jing, AU - Yuan,Li, AU - Xiao,Chunxia, AU - Wang,Yutang, AU - Liu,Xuebo, Y1 - 2013/02/11/ PY - 2013/2/1/entrez PY - 2013/2/1/pubmed PY - 2014/1/24/medline SP - 1509 EP - 20 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 61 IS - 7 N2 - Chicoric acid has been reported to possess various bioactivities. However, the antiobesity effects of chicoric acid remain poorly understood. In this study, we investigated the effects of chicoric acid on 3T3-L1 preadipocytes and its molecular mechanisms of apoptosis. Chicoric acid inhibited cell viability and induced apoptosis in 3T3-L1 preadipocytes which was characterized by chromatin condensation and poly ADP-ribose-polymerase (PARP) cleavage. Mitochondrial membrane potential (MMP) loss, Bax/Bcl-2 dysregulation, cytochrome c release, and caspase-3 activation were observed, indicating mitochondria-dependent apoptosis induced by chicoric acid. Furthermore, PI3K/Akt and MAPK (p38 MAPK, JNK, and ERK1/2) signaling pathways were involved in chicoric acid-induced apoptosis. The employment of protein kinase inhibitors LY294002, SB203580, SP600125, and U0126 revealed that PI3K/Akt signaling pathway interplayed with MAPK signaling pathways. Moreover, chicoric acid induced reactive oxygen species (ROS) generation. Pretreatment with the antioxidant N-acetylcysteine (NAC) significantly blocked cell death and changes of Akt and MAPK signalings induced by chicoric acid. In addition, chicoric acid down regulated HO-1 and COX-2 via the PI3K/Akt pathway. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/23363008/Chicoric_acid_induces_apoptosis_in_3T3_L1_preadipocytes_through_ROS_mediated_PI3K/Akt_and_MAPK_signaling_pathways_ L2 - https://doi.org/10.1021/jf3050268 DB - PRIME DP - Unbound Medicine ER -