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Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB.
World J Gastroenterol. 2013 Jan 21; 19(3):399-403.WJ

Abstract

AIM

To study the effects of Helicobacter pylori (H. pylori) tumor necrosis factor-α (TNF) inducing protein (Tip-α) on cytokine expression and its mechanism.

METHODS

We cloned Tip-α from the H. pylori strain 26695, transformed Escherichia coli with an expression plasmid, and then confirmed the expression product by Western blotting. Using different concentrations of Tip-α that affected SGC7901 and GES-1 cells at different times, we assessed cytokine levels using enzyme-linked immunosorbent assay. We blocked SGC7901 cells with pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of nuclear factor κB (NF-κB). We then detected interleukin (IL)-1β and TNF-α levels in SGC7901 cells.

RESULTS

Western blot analysis using an anti-Tip-α antibody revealed a 23-kDa protein, which indicated that recombinant Tip-α protein was recombined successfully. The levels of IL-1β, IL-8 and TNF-α were significantly higher following Tip-α interference, whether GES-1 cells or SGC-7901 cells were used (P < 0.05). However, the levels of cytokines (including IL-1β, IL-8 and TNF-α) secreted by SGC-7901 cells were greater than those secreted by GES-1 cells following treatment with Tip-α at the same concentration and for the same duration (P < 0.05). After blocking NF-κB with PDTC, the cells (GES-1 cells and SGC-7901 cells) underwent interference with Tip-α. We found that IL-1β and TNF-α levels were significantly decreased compared to cells that only underwent Tip-α interference (P < 0.05).

CONCLUSION

Tip-α plays an important role in cytokine expression through NF-κB.

Authors+Show Affiliations

Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23372364

Citation

Tang, Chun-Li, et al. "Helicobacter Pylori Tumor Necrosis Factor-α Inducing Protein Promotes Cytokine Expression Via Nuclear Factor-κB." World Journal of Gastroenterology, vol. 19, no. 3, 2013, pp. 399-403.
Tang CL, Hao B, Zhang GX, et al. Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB. World J Gastroenterol. 2013;19(3):399-403.
Tang, C. L., Hao, B., Zhang, G. X., Shi, R. H., & Cheng, W. F. (2013). Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB. World Journal of Gastroenterology, 19(3), 399-403. https://doi.org/10.3748/wjg.v19.i3.399
Tang CL, et al. Helicobacter Pylori Tumor Necrosis Factor-α Inducing Protein Promotes Cytokine Expression Via Nuclear Factor-κB. World J Gastroenterol. 2013 Jan 21;19(3):399-403. PubMed PMID: 23372364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB. AU - Tang,Chun-Li, AU - Hao,Bo, AU - Zhang,Guo-Xin, AU - Shi,Rui-Hua, AU - Cheng,Wen-Fang, PY - 2012/09/12/received PY - 2012/12/10/revised PY - 2012/12/20/accepted PY - 2013/2/2/entrez PY - 2013/2/2/pubmed PY - 2014/1/5/medline KW - Helicobacter pylori KW - Interleukin-1β KW - Interleukin-8 KW - Nuclear factor-κB KW - Tumor necrosis factor-α KW - Tumor necrosis factor-α inducing protein SP - 399 EP - 403 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 19 IS - 3 N2 - AIM: To study the effects of Helicobacter pylori (H. pylori) tumor necrosis factor-α (TNF) inducing protein (Tip-α) on cytokine expression and its mechanism. METHODS: We cloned Tip-α from the H. pylori strain 26695, transformed Escherichia coli with an expression plasmid, and then confirmed the expression product by Western blotting. Using different concentrations of Tip-α that affected SGC7901 and GES-1 cells at different times, we assessed cytokine levels using enzyme-linked immunosorbent assay. We blocked SGC7901 cells with pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of nuclear factor κB (NF-κB). We then detected interleukin (IL)-1β and TNF-α levels in SGC7901 cells. RESULTS: Western blot analysis using an anti-Tip-α antibody revealed a 23-kDa protein, which indicated that recombinant Tip-α protein was recombined successfully. The levels of IL-1β, IL-8 and TNF-α were significantly higher following Tip-α interference, whether GES-1 cells or SGC-7901 cells were used (P < 0.05). However, the levels of cytokines (including IL-1β, IL-8 and TNF-α) secreted by SGC-7901 cells were greater than those secreted by GES-1 cells following treatment with Tip-α at the same concentration and for the same duration (P < 0.05). After blocking NF-κB with PDTC, the cells (GES-1 cells and SGC-7901 cells) underwent interference with Tip-α. We found that IL-1β and TNF-α levels were significantly decreased compared to cells that only underwent Tip-α interference (P < 0.05). CONCLUSION: Tip-α plays an important role in cytokine expression through NF-κB. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/23372364/Helicobacter_pylori_tumor_necrosis_factor_α_inducing_protein_promotes_cytokine_expression_via_nuclear_factor_κB_ L2 - https://www.wjgnet.com/1007-9327/full/v19/i3/399.htm DB - PRIME DP - Unbound Medicine ER -