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Surface-enhanced Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at the single-cell level.
Anal Chem. 2013 Mar 05; 85(5):2809-16.AC

Abstract

Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool, enabling ultrahigh sensitive detection of analytes at low concentrations, even down to single-molecule level. Of particular note, in comparison to sufficient investigations on SERS-based detection of biomolecules (e.g., DNA and protein), there has been relatively scanty information regarding in vitro and in vivo detection. In this Article, we demonstrate a kind of SERS-active substrate, i.e., AgNPs-decorated silicon wafer (AgNPs@Si), as a high-performance in vitro sensing platform for single-cell detection of apoptotic cells. The AgNPs@Si yields highly reproducible SERS signals with an enhancement factor of ∼10(7). Remarkably, cellular experiments show that facile, noninvasive, label-free, and sensitive detection of apoptotic cells is readily realized using the high-performance SERS-active platform. Three kinds of apoptotic cells treated with apoptosis inducer are facilely and sensitively detected at the single-cell level, suggesting the exciting potential of AgNPs@Si for SERS-based in vitro analysis and detection.

Authors+Show Affiliations

Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou 215123, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23373817

Citation

Jiang, Xiangxu, et al. "Surface-enhanced Raman Scattering-based Sensing in Vitro: Facile and Label-free Detection of Apoptotic Cells at the Single-cell Level." Analytical Chemistry, vol. 85, no. 5, 2013, pp. 2809-16.
Jiang X, Jiang Z, Xu T, et al. Surface-enhanced Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at the single-cell level. Anal Chem. 2013;85(5):2809-16.
Jiang, X., Jiang, Z., Xu, T., Su, S., Zhong, Y., Peng, F., Su, Y., & He, Y. (2013). Surface-enhanced Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at the single-cell level. Analytical Chemistry, 85(5), 2809-16. https://doi.org/10.1021/ac303337b
Jiang X, et al. Surface-enhanced Raman Scattering-based Sensing in Vitro: Facile and Label-free Detection of Apoptotic Cells at the Single-cell Level. Anal Chem. 2013 Mar 5;85(5):2809-16. PubMed PMID: 23373817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at the single-cell level. AU - Jiang,Xiangxu, AU - Jiang,Ziyun, AU - Xu,Tingting, AU - Su,Shao, AU - Zhong,Yiling, AU - Peng,Fei, AU - Su,Yuanyuan, AU - He,Yao, Y1 - 2013/02/18/ PY - 2013/2/5/entrez PY - 2013/2/5/pubmed PY - 2013/11/20/medline SP - 2809 EP - 16 JF - Analytical chemistry JO - Anal Chem VL - 85 IS - 5 N2 - Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool, enabling ultrahigh sensitive detection of analytes at low concentrations, even down to single-molecule level. Of particular note, in comparison to sufficient investigations on SERS-based detection of biomolecules (e.g., DNA and protein), there has been relatively scanty information regarding in vitro and in vivo detection. In this Article, we demonstrate a kind of SERS-active substrate, i.e., AgNPs-decorated silicon wafer (AgNPs@Si), as a high-performance in vitro sensing platform for single-cell detection of apoptotic cells. The AgNPs@Si yields highly reproducible SERS signals with an enhancement factor of ∼10(7). Remarkably, cellular experiments show that facile, noninvasive, label-free, and sensitive detection of apoptotic cells is readily realized using the high-performance SERS-active platform. Three kinds of apoptotic cells treated with apoptosis inducer are facilely and sensitively detected at the single-cell level, suggesting the exciting potential of AgNPs@Si for SERS-based in vitro analysis and detection. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/23373817/Surface_enhanced_Raman_scattering_based_sensing_in_vitro:_facile_and_label_free_detection_of_apoptotic_cells_at_the_single_cell_level_ L2 - https://doi.org/10.1021/ac303337b DB - PRIME DP - Unbound Medicine ER -