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Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae).
Asian Pac J Trop Med. 2013 Mar; 6(3):180-7.AP

Abstract

OBJECTIVE

To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem.

METHODS

During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids, tannins and phytosterols. Antioxidant activity of the extract was determined by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity and DNA damage inhibition activity. Analysis of phenolic compounds was performed by Folin-Ciocalteau reagent method and gradient high performance liquid chromatography technique.

RESULTS

Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins, flavonoids, tannins and phytosterols as major phytochemical groups. The extract exhibited high 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC(50)= 110.74 μg/mL), metal chelating activity (IC(50)= 758.17 μg/mL), reducing power activity and DNA damage inhibition efficiency. The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid (3,4,5-OH), apigenin-7-O-glucoside (apigetrin), quercetin-3-O-rutinoside (rutin) and myricetin (3,5,7,3,4,5-OH) by high performance liquid chromatography analysis. The extract was found non toxic towards human erythrocytes in the hemolytic assay (IC(50) = 24.89 mg/mL).

CONCLUSIONS

These results conclud that A. lanata stem possesses high antioxidant activity and can be used for the development of natural and safe antioxidant compounds.

Authors+Show Affiliations

Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu - 632 014, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23375030

Citation

Kumar, Gaurav, et al. "Phytochemical Composition and in Vitro Antioxidant Activity of Aqueous Extract of Aerva Lanata (L.) Juss. Ex Schult. Stem (Amaranthaceae)." Asian Pacific Journal of Tropical Medicine, vol. 6, no. 3, 2013, pp. 180-7.
Kumar G, Karthik L, Rao KV. Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae). Asian Pac J Trop Med. 2013;6(3):180-7.
Kumar, G., Karthik, L., & Rao, K. V. (2013). Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae). Asian Pacific Journal of Tropical Medicine, 6(3), 180-7. https://doi.org/10.1016/S1995-7645(13)60020-6
Kumar G, Karthik L, Rao KV. Phytochemical Composition and in Vitro Antioxidant Activity of Aqueous Extract of Aerva Lanata (L.) Juss. Ex Schult. Stem (Amaranthaceae). Asian Pac J Trop Med. 2013;6(3):180-7. PubMed PMID: 23375030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae). AU - Kumar,Gaurav, AU - Karthik,Loganathan, AU - Rao,Kokati Vankata Bhaskara, PY - 2012/10/10/received PY - 2012/12/15/revised PY - 2013/01/15/accepted PY - 2013/2/5/entrez PY - 2013/2/5/pubmed PY - 2013/8/6/medline SP - 180 EP - 7 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 6 IS - 3 N2 - OBJECTIVE: To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem. METHODS: During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids, tannins and phytosterols. Antioxidant activity of the extract was determined by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity and DNA damage inhibition activity. Analysis of phenolic compounds was performed by Folin-Ciocalteau reagent method and gradient high performance liquid chromatography technique. RESULTS: Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins, flavonoids, tannins and phytosterols as major phytochemical groups. The extract exhibited high 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC(50)= 110.74 μg/mL), metal chelating activity (IC(50)= 758.17 μg/mL), reducing power activity and DNA damage inhibition efficiency. The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid (3,4,5-OH), apigenin-7-O-glucoside (apigetrin), quercetin-3-O-rutinoside (rutin) and myricetin (3,5,7,3,4,5-OH) by high performance liquid chromatography analysis. The extract was found non toxic towards human erythrocytes in the hemolytic assay (IC(50) = 24.89 mg/mL). CONCLUSIONS: These results conclud that A. lanata stem possesses high antioxidant activity and can be used for the development of natural and safe antioxidant compounds. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/23375030/Phytochemical_composition_and_in_vitro_antioxidant_activity_of_aqueous_extract_of_Aerva_lanata__L___Juss__ex_Schult__Stem__Amaranthaceae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(13)60020-6 DB - PRIME DP - Unbound Medicine ER -