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Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform.
Analyst. 2013 Mar 21; 138(6):1844-50.A

Abstract

A simple and sensitive label-free electrochemiluminescence (ECL) immunosensor based on the use of luminol functionalized gold nanoparticles (luminol-AuNPs) as antibody carriers and sensing platform is described for detecting the acute myocardial infarction biomarker cTnI. The ECL immunosensor was fabricated by the assembly of luminol-AuNPs conjugated with biotinylated antibodies against cTnI (biotin-anti-cTnI-luminol-AuNPs) with the streptavidin coated AuNPs (SA-AuNPs) modified Au electrode directly by virtue of the biotin-SA system. The fabricated sensing platform exhibited stable and strong ECL intensity and could be used for the recognition of target antigen. In the presence of cTnI, a decrease in the ECL intensity was observed. Direct detection of the ECL signal changes during antigen-antibody immunoreactions can be used for the quantification of cTnI. The ECL response exhibited a quite wide dynamic range from 1000 ng mL(-1) down to 0.1 ng mL(-1). The proposed method has been successfully applied in the detection of cTnI in real plasma samples. This protocol is simple, fast, sensitive, specific, stable and reliable. This work reveals that the luminol-AuNPs are excellent sensing platforms for the fabrication of simple and sensitive immunosensors. Moreover, the proposed strategy may also be extended for the detection of other biomarkers, which is of great application potential in clinical and pharmaceutical analysis.

Authors+Show Affiliations

Department of Chemistry, University of Science & Technology of China, Hefei, Anhui 230026, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23377497

Citation

Li, Fang, et al. "Label-free Electrochemiluminescence Immunosensor for Cardiac Troponin I Using Luminol Functionalized Gold Nanoparticles as a Sensing Platform." The Analyst, vol. 138, no. 6, 2013, pp. 1844-50.
Li F, Yu Y, Cui H, et al. Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. Analyst. 2013;138(6):1844-50.
Li, F., Yu, Y., Cui, H., Yang, D., & Bian, Z. (2013). Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. The Analyst, 138(6), 1844-50. https://doi.org/10.1039/c3an36805j
Li F, et al. Label-free Electrochemiluminescence Immunosensor for Cardiac Troponin I Using Luminol Functionalized Gold Nanoparticles as a Sensing Platform. Analyst. 2013 Mar 21;138(6):1844-50. PubMed PMID: 23377497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. AU - Li,Fang, AU - Yu,Yuqi, AU - Cui,Hua, AU - Yang,Di, AU - Bian,Zhiping, Y1 - 2013/02/04/ PY - 2013/2/5/entrez PY - 2013/2/5/pubmed PY - 2013/11/19/medline SP - 1844 EP - 50 JF - The Analyst JO - Analyst VL - 138 IS - 6 N2 - A simple and sensitive label-free electrochemiluminescence (ECL) immunosensor based on the use of luminol functionalized gold nanoparticles (luminol-AuNPs) as antibody carriers and sensing platform is described for detecting the acute myocardial infarction biomarker cTnI. The ECL immunosensor was fabricated by the assembly of luminol-AuNPs conjugated with biotinylated antibodies against cTnI (biotin-anti-cTnI-luminol-AuNPs) with the streptavidin coated AuNPs (SA-AuNPs) modified Au electrode directly by virtue of the biotin-SA system. The fabricated sensing platform exhibited stable and strong ECL intensity and could be used for the recognition of target antigen. In the presence of cTnI, a decrease in the ECL intensity was observed. Direct detection of the ECL signal changes during antigen-antibody immunoreactions can be used for the quantification of cTnI. The ECL response exhibited a quite wide dynamic range from 1000 ng mL(-1) down to 0.1 ng mL(-1). The proposed method has been successfully applied in the detection of cTnI in real plasma samples. This protocol is simple, fast, sensitive, specific, stable and reliable. This work reveals that the luminol-AuNPs are excellent sensing platforms for the fabrication of simple and sensitive immunosensors. Moreover, the proposed strategy may also be extended for the detection of other biomarkers, which is of great application potential in clinical and pharmaceutical analysis. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/23377497/Label_free_electrochemiluminescence_immunosensor_for_cardiac_troponin_I_using_luminol_functionalized_gold_nanoparticles_as_a_sensing_platform_ L2 - https://doi.org/10.1039/c3an36805j DB - PRIME DP - Unbound Medicine ER -