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Expression of dehydrin gene from Arctic Cerastium arcticum increases abiotic stress tolerance and enhances the fermentation capacity of a genetically engineered Saccharomyces cerevisiae laboratory strain.
Appl Microbiol Biotechnol. 2013 Oct; 97(20):8997-9009.AM

Abstract

We investigated Arctic plants to determine if they have a specific mechanism enabling them to adapt to extreme environments because they are subject to such conditions throughout their life cycles. Among the cell defense systems of the Arctic mouse-ear chickweed Cerastium arcticum, we identified a stress-responsive dehydrin gene CaDHN that belongs to the SK5 subclass and contains conserved regions with one S segment at the N-terminus and five K segments from the N-terminus to the C-terminus. To investigate the molecular properties of CaDHN, the yeast Saccharomyces was transformed with CaDHN. CaDHN-expressing transgenic yeast (TG) cells recovered more rapidly from challenge with exogenous stimuli, including oxidants (hydrogen peroxide, menadione, and tert-butyl hydroperoxide), high salinity, freezing and thawing, and metal (Zn(2+)), than wild-type (WT) cells. TG cells were sensitive to copper, cobalt, and sodium dodecyl sulfate. In addition, the cell survival of TG cells was higher than that of WT cells when cells at the mid-log and stationary stages were exposed to increased ethanol concentrations. There was a significant difference in cultures that have an ethanol content >16 %. During glucose-based batch fermentation at generally used (30 °C) and low (18 °C) temperatures, TG cells produced a higher alcohol concentration through improved cell survival. Specifically, the final alcohol concentrations were 13.3 and 13.2 % in TG cells during fermentation at 30 and 18 °C, respectively, whereas they were 10.2 and 9.4 %, respectively, in WT cells under the same fermentation conditions. An in vitro assay revealed that purified CaDHN acted as a reactive oxygen species scavenger by neutralizing H2O2 and a chaperone by preventing high temperature-mediated catalase inactivation. Taken together, our results show that CaDHN expression in transgenic yeast confers tolerance to various abiotic stresses by improving redox homeostasis and enhances fermentation capacity, especially at low temperatures (18 °C).

Authors+Show Affiliations

Advanced Bio-resource R&D Center, Department of Biology, College of Natural Sciences, Kyungpook National University, #1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23377791

Citation

Kim, Il-Sup, et al. "Expression of Dehydrin Gene From Arctic Cerastium Arcticum Increases Abiotic Stress Tolerance and Enhances the Fermentation Capacity of a Genetically Engineered Saccharomyces Cerevisiae Laboratory Strain." Applied Microbiology and Biotechnology, vol. 97, no. 20, 2013, pp. 8997-9009.
Kim IS, Kim HY, Kim YS, et al. Expression of dehydrin gene from Arctic Cerastium arcticum increases abiotic stress tolerance and enhances the fermentation capacity of a genetically engineered Saccharomyces cerevisiae laboratory strain. Appl Microbiol Biotechnol. 2013;97(20):8997-9009.
Kim, I. S., Kim, H. Y., Kim, Y. S., Choi, H. G., Kang, S. H., & Yoon, H. S. (2013). Expression of dehydrin gene from Arctic Cerastium arcticum increases abiotic stress tolerance and enhances the fermentation capacity of a genetically engineered Saccharomyces cerevisiae laboratory strain. Applied Microbiology and Biotechnology, 97(20), 8997-9009. https://doi.org/10.1007/s00253-013-4729-9
Kim IS, et al. Expression of Dehydrin Gene From Arctic Cerastium Arcticum Increases Abiotic Stress Tolerance and Enhances the Fermentation Capacity of a Genetically Engineered Saccharomyces Cerevisiae Laboratory Strain. Appl Microbiol Biotechnol. 2013;97(20):8997-9009. PubMed PMID: 23377791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of dehydrin gene from Arctic Cerastium arcticum increases abiotic stress tolerance and enhances the fermentation capacity of a genetically engineered Saccharomyces cerevisiae laboratory strain. AU - Kim,Il-Sup, AU - Kim,Hyun-Young, AU - Kim,Young-Saeng, AU - Choi,Han-Gu, AU - Kang,Sung-Ho, AU - Yoon,Ho-Sung, Y1 - 2013/02/03/ PY - 2012/10/18/received PY - 2013/01/15/accepted PY - 2013/01/13/revised PY - 2013/2/5/entrez PY - 2013/2/5/pubmed PY - 2014/4/17/medline SP - 8997 EP - 9009 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 97 IS - 20 N2 - We investigated Arctic plants to determine if they have a specific mechanism enabling them to adapt to extreme environments because they are subject to such conditions throughout their life cycles. Among the cell defense systems of the Arctic mouse-ear chickweed Cerastium arcticum, we identified a stress-responsive dehydrin gene CaDHN that belongs to the SK5 subclass and contains conserved regions with one S segment at the N-terminus and five K segments from the N-terminus to the C-terminus. To investigate the molecular properties of CaDHN, the yeast Saccharomyces was transformed with CaDHN. CaDHN-expressing transgenic yeast (TG) cells recovered more rapidly from challenge with exogenous stimuli, including oxidants (hydrogen peroxide, menadione, and tert-butyl hydroperoxide), high salinity, freezing and thawing, and metal (Zn(2+)), than wild-type (WT) cells. TG cells were sensitive to copper, cobalt, and sodium dodecyl sulfate. In addition, the cell survival of TG cells was higher than that of WT cells when cells at the mid-log and stationary stages were exposed to increased ethanol concentrations. There was a significant difference in cultures that have an ethanol content >16 %. During glucose-based batch fermentation at generally used (30 °C) and low (18 °C) temperatures, TG cells produced a higher alcohol concentration through improved cell survival. Specifically, the final alcohol concentrations were 13.3 and 13.2 % in TG cells during fermentation at 30 and 18 °C, respectively, whereas they were 10.2 and 9.4 %, respectively, in WT cells under the same fermentation conditions. An in vitro assay revealed that purified CaDHN acted as a reactive oxygen species scavenger by neutralizing H2O2 and a chaperone by preventing high temperature-mediated catalase inactivation. Taken together, our results show that CaDHN expression in transgenic yeast confers tolerance to various abiotic stresses by improving redox homeostasis and enhances fermentation capacity, especially at low temperatures (18 °C). SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/23377791/Expression_of_dehydrin_gene_from_Arctic_Cerastium_arcticum_increases_abiotic_stress_tolerance_and_enhances_the_fermentation_capacity_of_a_genetically_engineered_Saccharomyces_cerevisiae_laboratory_strain_ L2 - https://dx.doi.org/10.1007/s00253-013-4729-9 DB - PRIME DP - Unbound Medicine ER -