TY - JOUR T1 - Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts. AU - Apostolou,Anna, AU - Stagos,Dimitrios, AU - Galitsiou,Elissavet, AU - Spyrou,Argiris, AU - Haroutounian,Serko, AU - Portesis,Nikolaos, AU - Trizoglou,Ioanna, AU - Wallace Hayes,A, AU - Tsatsakis,Aristides M, AU - Kouretas,Dimitrios, Y1 - 2013/02/01/ PY - 2012/12/10/received PY - 2013/01/22/accepted PY - 2013/2/6/entrez PY - 2013/2/6/pubmed PY - 2014/10/7/medline KW - 1,1-diphenyl-2-picrylhydrazyl KW - 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) KW - 2,2′-azobis(2-methylpropionamidine) dihydrochloride KW - AAPH KW - ABTS KW - ABTS radical cation KW - ABTS(+) KW - Anticancer activity KW - Antioxidant KW - DMEM KW - DNA damage KW - DPPH KW - Dulbecco’s modified Eagle’s medium KW - Grape stem extracts KW - HPLC KW - HRP KW - PBS KW - Polyphenols KW - RCS KW - ROS KW - TPC KW - Vitis vinifera KW - high-performance liquid chromatography KW - horseradish peroxidase enzyme KW - phosphate buffer saline KW - radical scavenging capacity KW - reactive oxygen species KW - total phenolic content SP - 60 EP - 8 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 61 N2 - Grape extracts and wine have been studied widely due to their beneficial effects on human health. However, there are only few studies from grape stems extracts. Therefore, the main objective of the present study was the assessment in stem extracts from Greek Vitis vinifera varieties of the total polyphenolic content (TPC), the identification of the polyphenols present in them, and the evaluation of their antioxidant activity, protection against ROS-induced DNA damage and inhibition of liver (HepG2) and cervical (HeLa) cancer cell growth. The range of the TPC in grape stem extracts was from 345 to 584 mg GAE/g dry weight. Moreover, stem extracts contained different classes of polyphenols as flavonols, flavanols, procyanidins, phenolic acids and stilbenes. In DPPH and ABTS assays, the IC50 values of the stem extracts had an average of 7.8 ± 2.8 and 5.4 ± 2.6 μg/mL respectively. Also, all stem extracts inhibited OH- and ROO-induced DNA damage dose dependent with average IC50 values of 478 ± 217 and 1.15 ± 0.85 μg/mL respectively. Furthermore, stem extracts inhibited at low concentrations the growth of HepG2 and HeLa cancer cells with average IC50 values of 50 ± 12 and 32 ± 16 μg/mL respectively. The above activities of grape stem extracts were comparable to those of seed extracts. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23380202/Assessment_of_polyphenolic_content_antioxidant_activity_protection_against_ROS_induced_DNA_damage_and_anticancer_activity_of_Vitis_vinifera_stem_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(13)00067-7 DB - PRIME DP - Unbound Medicine ER -