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Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation.
PLoS One. 2013; 8(1):e55470.Plos

Abstract

FXYD proteins are novel regulators of Na(+)-K(+)-ATPase (NKA). In fish subjected to salinity challenges, NKA activity in osmoregulatory organs (e.g., gills) is a primary driving force for the many ion transport systems that act in concert to maintain a stable internal environment. Although teleostean FXYD proteins have been identified and investigated, previous studies focused on only a limited group of species. The purposes of the present study were to establish the brackish medaka (Oryzias dancena) as a potential saltwater fish model for osmoregulatory studies and to investigate the diversity of teleostean FXYD expression profiles by comparing two closely related euryhaline model teleosts, brackish medaka and Japanese medaka (O. latipes), upon exposure to salinity changes. Seven members of the FXYD protein family were identified in each medaka species, and the expression of most branchial fxyd genes was salinity-dependent. Among the cloned genes, fxyd11 was expressed specifically in the gills and at a significantly higher level than the other fxyd genes. In the brackish medaka, branchial fxyd11 expression was localized to the NKA-immunoreactive cells in gill epithelia. Furthermore, the FXYD11 protein interacted with the NKA α-subunit and was expressed at a higher level in freshwater-acclimated individuals relative to fish in other salinity groups. The protein sequences and tissue distributions of the FXYD proteins were very similar between the two medaka species, but different expression profiles were observed upon salinity challenge for most branchial fxyd genes. Salinity changes produced different effects on the FXYD11 and NKA α-subunit expression patterns in the gills of the brackish medaka. To our knowledge, this report is the first to focus on FXYD expression in the gills of closely related euryhaline teleosts. Given the advantages conferred by the well-developed Japanese medaka system, we propose the brackish medaka as a saltwater fish model for osmoregulatory studies.

Authors+Show Affiliations

Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23383199

Citation

Yang, Wen-Kai, et al. "Expression Profiles of Branchial FXYD Proteins in the Brackish Medaka Oryzias Dancena: a Potential Saltwater Fish Model for Studies of Osmoregulation." PloS One, vol. 8, no. 1, 2013, pp. e55470.
Yang WK, Kang CK, Chang CH, et al. Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation. PLoS One. 2013;8(1):e55470.
Yang, W. K., Kang, C. K., Chang, C. H., Hsu, A. D., Lee, T. H., & Hwang, P. P. (2013). Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation. PloS One, 8(1), e55470. https://doi.org/10.1371/journal.pone.0055470
Yang WK, et al. Expression Profiles of Branchial FXYD Proteins in the Brackish Medaka Oryzias Dancena: a Potential Saltwater Fish Model for Studies of Osmoregulation. PLoS One. 2013;8(1):e55470. PubMed PMID: 23383199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation. AU - Yang,Wen-Kai, AU - Kang,Chao-Kai, AU - Chang,Chia-Hao, AU - Hsu,An-Di, AU - Lee,Tsung-Han, AU - Hwang,Pung-Pung, Y1 - 2013/01/31/ PY - 2012/09/14/received PY - 2012/12/23/accepted PY - 2013/2/6/entrez PY - 2013/2/6/pubmed PY - 2013/7/19/medline SP - e55470 EP - e55470 JF - PloS one JO - PLoS One VL - 8 IS - 1 N2 - FXYD proteins are novel regulators of Na(+)-K(+)-ATPase (NKA). In fish subjected to salinity challenges, NKA activity in osmoregulatory organs (e.g., gills) is a primary driving force for the many ion transport systems that act in concert to maintain a stable internal environment. Although teleostean FXYD proteins have been identified and investigated, previous studies focused on only a limited group of species. The purposes of the present study were to establish the brackish medaka (Oryzias dancena) as a potential saltwater fish model for osmoregulatory studies and to investigate the diversity of teleostean FXYD expression profiles by comparing two closely related euryhaline model teleosts, brackish medaka and Japanese medaka (O. latipes), upon exposure to salinity changes. Seven members of the FXYD protein family were identified in each medaka species, and the expression of most branchial fxyd genes was salinity-dependent. Among the cloned genes, fxyd11 was expressed specifically in the gills and at a significantly higher level than the other fxyd genes. In the brackish medaka, branchial fxyd11 expression was localized to the NKA-immunoreactive cells in gill epithelia. Furthermore, the FXYD11 protein interacted with the NKA α-subunit and was expressed at a higher level in freshwater-acclimated individuals relative to fish in other salinity groups. The protein sequences and tissue distributions of the FXYD proteins were very similar between the two medaka species, but different expression profiles were observed upon salinity challenge for most branchial fxyd genes. Salinity changes produced different effects on the FXYD11 and NKA α-subunit expression patterns in the gills of the brackish medaka. To our knowledge, this report is the first to focus on FXYD expression in the gills of closely related euryhaline teleosts. Given the advantages conferred by the well-developed Japanese medaka system, we propose the brackish medaka as a saltwater fish model for osmoregulatory studies. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23383199/Expression_profiles_of_branchial_FXYD_proteins_in_the_brackish_medaka_Oryzias_dancena:_a_potential_saltwater_fish_model_for_studies_of_osmoregulation_ L2 - https://dx.plos.org/10.1371/journal.pone.0055470 DB - PRIME DP - Unbound Medicine ER -