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[Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats].
Zhen Ci Yan Jiu 2012; 37(6):470-5ZC

Abstract

OBJECTIVE

To observe the effects of acupuncture of "Baihui" (GV 20) and "Zusanli" (ST 36) on the expression of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha proteins in the bilateral cerebral tissue in cerebral ischemia reperfusion injury (CI/RI) rats, so as to explore its mechanism underlying improving CI.

METHODS

Seventy-eight SD rats were randomized into sham-operation (control) group (n = 6), model group (n = 36) and acupuncture group (n = 36), and the latter two groups were further divided into 12, 24, 48, 72, 96 and 144 h subgroups (n = 6 in each subgroup) according to the reperfusion time. Acupuncture stimulation was applied to GV 20 and left ST 36 for 20 min at the required time-points after successful operation, once a day. The expression of IL-1beta and TNF-alpha in bilateral brain tissue was examined by immunohistochemistry.

RESULTS

Immunohistochemical staining showed that the expression of cerebral IL-1beta protein in cerebral ischemia regions exhibited a double-peak, and the peak time in the model group was at 48 h and 96 h, while the expression of TNF-alpha protein in cerebral ischemia regions exhibited a single-peak, and the peak time in both model and acupuncture groups was at 72 h. In comparison with the normal group and sham group, cerebral IL-1beta and TNF-alpha protein expression levels of both ischemic and non-ischemic regions were increased significantly in the model group (P < 0.05). Compared to the model group, the expression levels of cerebral IL-1beta and TNF-alpha of both ischemic and non-ischemic regions in the acupuncture group at most of the time-points after CI/RI were decreased obviously (P < 0.05).

CONCLUSION

Acupuncture can effectively suppress the expression of IL-1beta and TNF-alpha proteins in bilateral ischemic and non-ischemic cerebral regions in CI/RI rats,suggesting an anti-inflammatory effect of acupuncture intervention.

Authors+Show Affiliations

Chinese Academy of Medical Science, Peking Union Medical College, Department of Chinese Medicine, Peking Union Medical College Hospital, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23383456

Citation

Chen, Su-Hui, et al. "[Effects of Acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) On Expression of Cerebral IL-1beta and TNF-alpha Proteins in Cerebral Ischemia Reperfusion Injury Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 6, 2012, pp. 470-5.
Chen SH, Sun H, Xu H, et al. [Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats]. Zhen Ci Yan Jiu. 2012;37(6):470-5.
Chen, S. H., Sun, H., Xu, H., Zhang, Y. M., Gao, Y., & Li, S. (2012). [Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(6), pp. 470-5.
Chen SH, et al. [Effects of Acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) On Expression of Cerebral IL-1beta and TNF-alpha Proteins in Cerebral Ischemia Reperfusion Injury Rats]. Zhen Ci Yan Jiu. 2012;37(6):470-5. PubMed PMID: 23383456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats]. AU - Chen,Su-Hui, AU - Sun,Hua, AU - Xu,Hong, AU - Zhang,Ya-Min, AU - Gao,Yang, AU - Li,Sha, PY - 2013/2/7/entrez PY - 2013/2/7/pubmed PY - 2013/3/21/medline SP - 470 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 6 N2 - OBJECTIVE: To observe the effects of acupuncture of "Baihui" (GV 20) and "Zusanli" (ST 36) on the expression of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha proteins in the bilateral cerebral tissue in cerebral ischemia reperfusion injury (CI/RI) rats, so as to explore its mechanism underlying improving CI. METHODS: Seventy-eight SD rats were randomized into sham-operation (control) group (n = 6), model group (n = 36) and acupuncture group (n = 36), and the latter two groups were further divided into 12, 24, 48, 72, 96 and 144 h subgroups (n = 6 in each subgroup) according to the reperfusion time. Acupuncture stimulation was applied to GV 20 and left ST 36 for 20 min at the required time-points after successful operation, once a day. The expression of IL-1beta and TNF-alpha in bilateral brain tissue was examined by immunohistochemistry. RESULTS: Immunohistochemical staining showed that the expression of cerebral IL-1beta protein in cerebral ischemia regions exhibited a double-peak, and the peak time in the model group was at 48 h and 96 h, while the expression of TNF-alpha protein in cerebral ischemia regions exhibited a single-peak, and the peak time in both model and acupuncture groups was at 72 h. In comparison with the normal group and sham group, cerebral IL-1beta and TNF-alpha protein expression levels of both ischemic and non-ischemic regions were increased significantly in the model group (P < 0.05). Compared to the model group, the expression levels of cerebral IL-1beta and TNF-alpha of both ischemic and non-ischemic regions in the acupuncture group at most of the time-points after CI/RI were decreased obviously (P < 0.05). CONCLUSION: Acupuncture can effectively suppress the expression of IL-1beta and TNF-alpha proteins in bilateral ischemic and non-ischemic cerebral regions in CI/RI rats,suggesting an anti-inflammatory effect of acupuncture intervention. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/23383456/[Effects_of_acupuncture_of_"Baihui"_GV_20__and_"Zusanli"_ST_36__on_expression_of_cerebral_IL_1beta_and_TNF_alpha_proteins_in_cerebral_ischemia_reperfusion_injury_rats]_ L2 - https://medlineplus.gov/acupuncture.html DB - PRIME DP - Unbound Medicine ER -