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[Effects of electroacupuncture on microvascular ultrastructure and VEGF expression of the right cerebral cortex in focal cerebral ischemia/reperfusion injury rats].
Zhen Ci Yan Jiu. 2012 Dec; 37(6):476-81.ZC

Abstract

OBJECTIVE

To study the protective effects of electroacupuncture (EA) on the cortical microvasculature in rats with focal cerebral ischemia/reperfusion injury (CI/RI).

METHODS

Eighty SD rats were randomly divided into: normal group (n = 8), sham-operation (sham) group (n = 8), model group (n = 32) and EA group (n = 32). The II/II model was established by occlusion of the middle cerebral artery for 30 min and reperfusion for 1 day (d), 2 d, 4 d and 8 d respectively. EA was applied to "Baihui" (GV20), "Shuigou" (GV 26) and bilateral "Zusanli" (ST 36) areas for 30 min, once daily for 1 d, 2 d, 4 d and 8 d. The right cortical microvascular ultrastructure was examined with transmission electron microscopy and cerebral vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative real-time PCR.

RESULTS

The microvascular ultrastructure of the right cerebral cortex was severely injured in rats of the model group whereas greatly improved in rats of EA group. VEGF mRNA expression of the right cerebral cortex was increased significantly in the model group (P < 0.05, P < 0.01) and further upregulated in the EA group (P < 0.01).

CONCLUSION

EA intervention can alleviate the injury of microvascular ultrastructure of focal ischemic cerebral tissue and upregulate cerebral VEGF mRNA expression, suggesting a role of EA in protecting the ischemic brain tissues by facilitating the angiogenesis of capillary vessels and the function reconstruction of the damaged microvasculature.

Authors+Show Affiliations

Department of Chinese Medicine, Affiliated Tianyou Hospital of Wuhan University of Science and Technology, Wuhan 430064, China.No affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23383457

Citation

Mao, Qing-Ju, and Bang-Guo Chen. "[Effects of Electroacupuncture On Microvascular Ultrastructure and VEGF Expression of the Right Cerebral Cortex in Focal Cerebral Ischemia/reperfusion Injury Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 6, 2012, pp. 476-81.
Mao QJ, Chen BG. [Effects of electroacupuncture on microvascular ultrastructure and VEGF expression of the right cerebral cortex in focal cerebral ischemia/reperfusion injury rats]. Zhen Ci Yan Jiu. 2012;37(6):476-81.
Mao, Q. J., & Chen, B. G. (2012). [Effects of electroacupuncture on microvascular ultrastructure and VEGF expression of the right cerebral cortex in focal cerebral ischemia/reperfusion injury rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(6), 476-81.
Mao QJ, Chen BG. [Effects of Electroacupuncture On Microvascular Ultrastructure and VEGF Expression of the Right Cerebral Cortex in Focal Cerebral Ischemia/reperfusion Injury Rats]. Zhen Ci Yan Jiu. 2012;37(6):476-81. PubMed PMID: 23383457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on microvascular ultrastructure and VEGF expression of the right cerebral cortex in focal cerebral ischemia/reperfusion injury rats]. AU - Mao,Qing-Ju, AU - Chen,Bang-Guo, PY - 2013/2/7/entrez PY - 2013/2/7/pubmed PY - 2013/3/21/medline SP - 476 EP - 81 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 6 N2 - OBJECTIVE: To study the protective effects of electroacupuncture (EA) on the cortical microvasculature in rats with focal cerebral ischemia/reperfusion injury (CI/RI). METHODS: Eighty SD rats were randomly divided into: normal group (n = 8), sham-operation (sham) group (n = 8), model group (n = 32) and EA group (n = 32). The II/II model was established by occlusion of the middle cerebral artery for 30 min and reperfusion for 1 day (d), 2 d, 4 d and 8 d respectively. EA was applied to "Baihui" (GV20), "Shuigou" (GV 26) and bilateral "Zusanli" (ST 36) areas for 30 min, once daily for 1 d, 2 d, 4 d and 8 d. The right cortical microvascular ultrastructure was examined with transmission electron microscopy and cerebral vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative real-time PCR. RESULTS: The microvascular ultrastructure of the right cerebral cortex was severely injured in rats of the model group whereas greatly improved in rats of EA group. VEGF mRNA expression of the right cerebral cortex was increased significantly in the model group (P < 0.05, P < 0.01) and further upregulated in the EA group (P < 0.01). CONCLUSION: EA intervention can alleviate the injury of microvascular ultrastructure of focal ischemic cerebral tissue and upregulate cerebral VEGF mRNA expression, suggesting a role of EA in protecting the ischemic brain tissues by facilitating the angiogenesis of capillary vessels and the function reconstruction of the damaged microvasculature. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/23383457/[Effects_of_electroacupuncture_on_microvascular_ultrastructure_and_VEGF_expression_of_the_right_cerebral_cortex_in_focal_cerebral_ischemia/reperfusion_injury_rats]_ DB - PRIME DP - Unbound Medicine ER -