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Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook.
J Ethnopharmacol. 2013 Mar 27; 146(2):505-14.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Potentilla fulgens (Wall.) ex Hook. (Rosaceae) is a potent medicinal plant of the Western Himalayas, known under the name "Himalayan Cinquefoil or Bajradanti", and has been used traditionally to treat ailments including peptic ulcers, mouth ulcers, diarrhea, diabetes and cancer.

OBJECTIVE

The aim of the present study was to scientifically evaluate the gastric-ulcer protective effect of P. fulgens ethanolic root extract (EPF) on experimental rats.

MATERIAL AND METHODS

The gastroprotective activity of EPF was evaluated on four gastric-ulcer models such as pyloric ligation (PL), ethanol (EtOH), cold restrain stress (CRS) and aspirin (ASP)-induced gastric ulcers. The gastric acid obtained from 4h PL-induced gastric ulcer rats was determined for total volume content, pH and total acid-pepsin output. Total carbohydrates and protein ratio, expressed as index of mucin activity, and DNA content were estimated in the gastric juice and gastric mucosal tissue. The microvascular permeability, H(+)K(+)-ATPase activity, gastric mucus and histamine content were also determined. The levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione) and malondialdehyde in the stomach tissue (mucosal scrapings) were quantified. A histopathological study of the stomach was evaluated using eosin-haematoxylin stain.

RESULTS

EPF (200-400mg/kg, p.o.) showed significant protection against acute gastric-ulcer induced by EtOH, PL and CRS (400mg/kg, p.o.), but was found to be ineffective against ASP-induced ulcerogens. The effect of EPF on gastric juice studies in 4h PL rats significantly produced an increased level in gastric pH, whereas the effect on gastric volume and acid-pepsin output was observed to decrease significantly. However, EPF was found to have no significant effect on the defensive factors, thus revealing its antisecretory property by inhibiting the aggressive factors. EPF, significantly decreased the histamine level, inhibited the H(+)K(+)-ATPase activity and prevented the microvascular injury caused by ethanol in the rat stomach. Moreover, it was also observed to have antioxidant effects by producing a significant increase in the levels of SOD, CAT, and GSH and decreased the LPO activity. Histopathological studies showed that EPF significantly prevented gastric lesions caused by ethanol.

CONCLUSIONS

The present study showed that EPF has potent gastroprotective and antisecretory effects, thus justifying the traditional usage of this herb to treat gastric ulcers.

Authors+Show Affiliations

Department of Pharmaceutics, Indian Institute of Technology, Banaras Hindu University, Varanasi 221 005, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23384786

Citation

Laloo, Damiki, et al. "Gastroprotective Activity of Ethanolic Root Extract of Potentilla Fulgens Wall. Ex Hook." Journal of Ethnopharmacology, vol. 146, no. 2, 2013, pp. 505-14.
Laloo D, Prasad SK, Krishnamurthy S, et al. Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. J Ethnopharmacol. 2013;146(2):505-14.
Laloo, D., Prasad, S. K., Krishnamurthy, S., & Hemalatha, S. (2013). Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. Journal of Ethnopharmacology, 146(2), 505-14. https://doi.org/10.1016/j.jep.2013.01.015
Laloo D, et al. Gastroprotective Activity of Ethanolic Root Extract of Potentilla Fulgens Wall. Ex Hook. J Ethnopharmacol. 2013 Mar 27;146(2):505-14. PubMed PMID: 23384786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. AU - Laloo,Damiki, AU - Prasad,Satyendra K, AU - Krishnamurthy,Sairam, AU - Hemalatha,Siva, Y1 - 2013/02/04/ PY - 2012/10/05/received PY - 2012/12/31/revised PY - 2013/01/12/accepted PY - 2013/2/7/entrez PY - 2013/2/7/pubmed PY - 2013/8/29/medline SP - 505 EP - 14 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 146 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Potentilla fulgens (Wall.) ex Hook. (Rosaceae) is a potent medicinal plant of the Western Himalayas, known under the name "Himalayan Cinquefoil or Bajradanti", and has been used traditionally to treat ailments including peptic ulcers, mouth ulcers, diarrhea, diabetes and cancer. OBJECTIVE: The aim of the present study was to scientifically evaluate the gastric-ulcer protective effect of P. fulgens ethanolic root extract (EPF) on experimental rats. MATERIAL AND METHODS: The gastroprotective activity of EPF was evaluated on four gastric-ulcer models such as pyloric ligation (PL), ethanol (EtOH), cold restrain stress (CRS) and aspirin (ASP)-induced gastric ulcers. The gastric acid obtained from 4h PL-induced gastric ulcer rats was determined for total volume content, pH and total acid-pepsin output. Total carbohydrates and protein ratio, expressed as index of mucin activity, and DNA content were estimated in the gastric juice and gastric mucosal tissue. The microvascular permeability, H(+)K(+)-ATPase activity, gastric mucus and histamine content were also determined. The levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione) and malondialdehyde in the stomach tissue (mucosal scrapings) were quantified. A histopathological study of the stomach was evaluated using eosin-haematoxylin stain. RESULTS: EPF (200-400mg/kg, p.o.) showed significant protection against acute gastric-ulcer induced by EtOH, PL and CRS (400mg/kg, p.o.), but was found to be ineffective against ASP-induced ulcerogens. The effect of EPF on gastric juice studies in 4h PL rats significantly produced an increased level in gastric pH, whereas the effect on gastric volume and acid-pepsin output was observed to decrease significantly. However, EPF was found to have no significant effect on the defensive factors, thus revealing its antisecretory property by inhibiting the aggressive factors. EPF, significantly decreased the histamine level, inhibited the H(+)K(+)-ATPase activity and prevented the microvascular injury caused by ethanol in the rat stomach. Moreover, it was also observed to have antioxidant effects by producing a significant increase in the levels of SOD, CAT, and GSH and decreased the LPO activity. Histopathological studies showed that EPF significantly prevented gastric lesions caused by ethanol. CONCLUSIONS: The present study showed that EPF has potent gastroprotective and antisecretory effects, thus justifying the traditional usage of this herb to treat gastric ulcers. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23384786/Gastroprotective_activity_of_ethanolic_root_extract_of_Potentilla_fulgens_Wall__ex_Hook_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00037-8 DB - PRIME DP - Unbound Medicine ER -