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Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice.
Phytopathology. 2013 Jun; 103(6):594-9.P

Abstract

The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China.

Authors+Show Affiliations

Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23384860

Citation

Lv, Qiming, et al. "Functional Analysis of Pid3-A4, an Ortholog of Rice Blast Resistance Gene Pid3 Revealed By Allele Mining in Common Wild Rice." Phytopathology, vol. 103, no. 6, 2013, pp. 594-9.
Lv Q, Xu X, Shang J, et al. Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice. Phytopathology. 2013;103(6):594-9.
Lv, Q., Xu, X., Shang, J., Jiang, G., Pang, Z., Zhou, Z., Wang, J., Liu, Y., Li, T., Li, X., Xu, J., Cheng, Z., Zhao, X., Li, S., & Zhu, L. (2013). Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice. Phytopathology, 103(6), 594-9. https://doi.org/10.1094/PHYTO-10-12-0260-R
Lv Q, et al. Functional Analysis of Pid3-A4, an Ortholog of Rice Blast Resistance Gene Pid3 Revealed By Allele Mining in Common Wild Rice. Phytopathology. 2013;103(6):594-9. PubMed PMID: 23384860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice. AU - Lv,Qiming, AU - Xu,Xiao, AU - Shang,Junjun, AU - Jiang,Guanghuai, AU - Pang,Zhiqian, AU - Zhou,Zhuangzhi, AU - Wang,Jing, AU - Liu,Ya, AU - Li,Ting, AU - Li,Xiaobing, AU - Xu,Jichen, AU - Cheng,Zhukuan, AU - Zhao,Xianfeng, AU - Li,Shigui, AU - Zhu,Lihuang, PY - 2013/2/7/entrez PY - 2013/2/7/pubmed PY - 2013/7/13/medline SP - 594 EP - 9 JF - Phytopathology JO - Phytopathology VL - 103 IS - 6 N2 - The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/23384860/Functional_analysis_of_Pid3_A4_an_ortholog_of_rice_blast_resistance_gene_Pid3_revealed_by_allele_mining_in_common_wild_rice_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-12-0260-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -