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Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies.
Methods Mol Biol. 2013; 984:139-51.MM

Abstract

In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgun-like methodology used for proteomic analysis. Particularly, CE-MS using a time-of-flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Simó C
Laboratory of Foodomics, CIAL, CSIC, Madrid, Spain.
Cifuentes A
No affiliation info available
Kašička V
No affiliation info available

MeSH

DipeptidesElectrophoresis, CapillaryLimit of DetectionPeptide FragmentsPlant ProteinsPlants, Genetically ModifiedProteolysisProteomeProteomicsSeedsSoybeansSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryVicia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23386342

Citation

Simó, Carolina, et al. "Capillary Electrophoresis-mass Spectrometry for Peptide Analysis: Target-based Approaches and Proteomics/peptidomics Strategies." Methods in Molecular Biology (Clifton, N.J.), vol. 984, 2013, pp. 139-51.
Simó C, Cifuentes A, Kašička V. Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies. Methods Mol Biol. 2013;984:139-51.
Simó, C., Cifuentes, A., & Kašička, V. (2013). Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies. Methods in Molecular Biology (Clifton, N.J.), 984, 139-51. https://doi.org/10.1007/978-1-62703-296-4_11
Simó C, Cifuentes A, Kašička V. Capillary Electrophoresis-mass Spectrometry for Peptide Analysis: Target-based Approaches and Proteomics/peptidomics Strategies. Methods Mol Biol. 2013;984:139-51. PubMed PMID: 23386342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies. AU - Simó,Carolina, AU - Cifuentes,Alejandro, AU - Kašička,Václav, PY - 2013/2/7/entrez PY - 2013/2/7/pubmed PY - 2013/7/19/medline SP - 139 EP - 51 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 984 N2 - In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgun-like methodology used for proteomic analysis. Particularly, CE-MS using a time-of-flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/23386342/Capillary_electrophoresis_mass_spectrometry_for_Peptide_analysis:_target_based_approaches_and_proteomics/peptidomics_strategies_ L2 - https://dx.doi.org/10.1007/978-1-62703-296-4_11 DB - PRIME DP - Unbound Medicine ER -