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[Simulated weightlessness inhibits antitumor immunity of T lymphocytes in melanoma-bearing mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Feb; 29(2):127-31.XB

Abstract

OBJECTIVE

To investigate the effects of simulated weightlessness on antitumor immunity of T lymphocytes in mice.

METHODS

The malignant melanoma was xenografted by subcutaneous injection of B16 cells into the right hind limb of every mouse. The mice suspended by tail at a -15 degree to 20 degree head-down tilt were used as simulated weightlessness models. The effects of simulated weightlessness on tumor volume and survival time were observed. T the numbers of leucocytes, lymphocytes and T lymphocyte subsets in peripheral blood of tumor-bearing mice under simulated weightlessness were monitored by an automatic hemacytometer and a flow cytometer. The effects of simulated weightlessness on the production of IL-2, TNF-α and IFN-γ in T lymphocytes and the cytotoxicities of tumor-specific CTLs to tumor cells were analyzed by ELISA and LDH release.

RESULTS

Compared with control group, the tumors grew faster, the survival times were shorter, the number of lymphocytes, the ratio of lymphocytes, CD3(+);, CD4(+);/CD3(+); and CD8(+);/CD3(+); T lymphocytes in peripheral blood dropped, and the proliferation of splenic T lymphocytes induced by mitogen was reduced (P<0.05 or P<0.01) in the simulated weightlessness group. The production of IL-2, TNF-α and IFN-γ induced by tumor cells and cytotoxicities of tumor-specific CTLs to tumor cells were inhibited in mice under simulated weightlessness (P<0.05 or P<0.01).

CONCLUSION

Simulated weightlessness inhibits antitumor immunity of T lymphocytes.

Authors+Show Affiliations

Department of Pathology and Experimental Medicine, 306th Hospital of PLA, Beijing 100101, China. sishy306@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

23388329

Citation

Si, Shaoyan, et al. "[Simulated Weightlessness Inhibits Antitumor Immunity of T Lymphocytes in Melanoma-bearing Mice]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 29, no. 2, 2013, pp. 127-31.
Si S, Song S, Shi L, et al. [Simulated weightlessness inhibits antitumor immunity of T lymphocytes in melanoma-bearing mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013;29(2):127-31.
Si, S., Song, S., Shi, L., Liu, J., Xu, B., Yi, Y., Tan, X., & Zhang, J. (2013). [Simulated weightlessness inhibits antitumor immunity of T lymphocytes in melanoma-bearing mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 29(2), 127-31.
Si S, et al. [Simulated Weightlessness Inhibits Antitumor Immunity of T Lymphocytes in Melanoma-bearing Mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013;29(2):127-31. PubMed PMID: 23388329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simulated weightlessness inhibits antitumor immunity of T lymphocytes in melanoma-bearing mice]. AU - Si,Shaoyan, AU - Song,Shujun, AU - Shi,Liang, AU - Liu,Junli, AU - Xu,Bingxin, AU - Yi,Yong, AU - Tan,Xiaoqing, AU - Zhang,Jianzhong, PY - 2013/2/8/entrez PY - 2013/2/8/pubmed PY - 2014/1/18/medline SP - 127 EP - 31 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 29 IS - 2 N2 - OBJECTIVE: To investigate the effects of simulated weightlessness on antitumor immunity of T lymphocytes in mice. METHODS: The malignant melanoma was xenografted by subcutaneous injection of B16 cells into the right hind limb of every mouse. The mice suspended by tail at a -15 degree to 20 degree head-down tilt were used as simulated weightlessness models. The effects of simulated weightlessness on tumor volume and survival time were observed. T the numbers of leucocytes, lymphocytes and T lymphocyte subsets in peripheral blood of tumor-bearing mice under simulated weightlessness were monitored by an automatic hemacytometer and a flow cytometer. The effects of simulated weightlessness on the production of IL-2, TNF-α and IFN-γ in T lymphocytes and the cytotoxicities of tumor-specific CTLs to tumor cells were analyzed by ELISA and LDH release. RESULTS: Compared with control group, the tumors grew faster, the survival times were shorter, the number of lymphocytes, the ratio of lymphocytes, CD3(+);, CD4(+);/CD3(+); and CD8(+);/CD3(+); T lymphocytes in peripheral blood dropped, and the proliferation of splenic T lymphocytes induced by mitogen was reduced (P<0.05 or P<0.01) in the simulated weightlessness group. The production of IL-2, TNF-α and IFN-γ induced by tumor cells and cytotoxicities of tumor-specific CTLs to tumor cells were inhibited in mice under simulated weightlessness (P<0.05 or P<0.01). CONCLUSION: Simulated weightlessness inhibits antitumor immunity of T lymphocytes. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/23388329/[Simulated_weightlessness_inhibits_antitumor_immunity_of_T_lymphocytes_in_melanoma_bearing_mice]_ L2 - https://medlineplus.gov/bodyweight.html DB - PRIME DP - Unbound Medicine ER -