Tags

Type your tag names separated by a space and hit enter

Osthole ameliorates renal ischemia-reperfusion injury in rats.
J Surg Res. 2013 Jul; 183(1):347-54.JS

Abstract

BACKGROUND

Renal ischemia-reperfusion (I/R) injury is a major cause of acute kidney injury. The pathogenetic mechanisms underlying I/R injury involve oxidative stress and apoptosis. Osthole, a natural coumarin derivative, has been reported to possess antioxidant and antiapoptotic activities. This study aimed to investigate the potential effects of osthole on renal I/R injury in an in vivo rat model.

MATERIALS AND METHODS

We induced renal I/R injury by clamping the left renal artery for 45 min followed by reperfusion, along with a contralateral nephrectomy. We randomly assigned 54 rats to three groups (18 rats/group): sham-operated, vehicle-treated I/R, and osthole-treated I/R. We treated rats intraperitoneally with osthole (40 mg/kg) or vehicle (40 mg/kg) 30 min before renal ischemia. We harvested serum and kidneys at 1, 6, and 24 h after reperfusion. Renal function and histological changes were assessed. We also determined markers of oxidative stress and cell apoptosis in kidneys.

RESULTS

Osthole treatment significantly attenuated renal dysfunction and histologic damage induced by I/R injury. The I/R-induced elevation in kidney malondialdehyde level decreased, whereas reduced kidney superoxide dismutase and catalase activities were markedly increased. Moreover, osthole-treated rats had a dramatic decrease in apoptotic tubular cells, along with a decrease in caspase-3 and an increase in the Bcl-2/Bax ratio.

CONCLUSIONS

Osthole treatment protects murine kidney from renal I/R injury by suppressing oxidative stress and cell apoptosis. Thus, osthole may represent a novel practical strategy to prevent renal I/R injury.

Authors+Show Affiliations

Department of Urology, Peking University Third Hospital, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23391166

Citation

Zheng, Yi, et al. "Osthole Ameliorates Renal Ischemia-reperfusion Injury in Rats." The Journal of Surgical Research, vol. 183, no. 1, 2013, pp. 347-54.
Zheng Y, Lu M, Ma L, et al. Osthole ameliorates renal ischemia-reperfusion injury in rats. J Surg Res. 2013;183(1):347-54.
Zheng, Y., Lu, M., Ma, L., Zhang, S., Qiu, M., & Wang, Y. (2013). Osthole ameliorates renal ischemia-reperfusion injury in rats. The Journal of Surgical Research, 183(1), 347-54. https://doi.org/10.1016/j.jss.2013.01.008
Zheng Y, et al. Osthole Ameliorates Renal Ischemia-reperfusion Injury in Rats. J Surg Res. 2013;183(1):347-54. PubMed PMID: 23391166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osthole ameliorates renal ischemia-reperfusion injury in rats. AU - Zheng,Yi, AU - Lu,Min, AU - Ma,Lulin, AU - Zhang,Shudong, AU - Qiu,Min, AU - Wang,Yunpeng, Y1 - 2013/02/01/ PY - 2012/11/21/received PY - 2012/12/29/revised PY - 2013/01/03/accepted PY - 2013/2/9/entrez PY - 2013/2/9/pubmed PY - 2013/8/28/medline SP - 347 EP - 54 JF - The Journal of surgical research JO - J Surg Res VL - 183 IS - 1 N2 - BACKGROUND: Renal ischemia-reperfusion (I/R) injury is a major cause of acute kidney injury. The pathogenetic mechanisms underlying I/R injury involve oxidative stress and apoptosis. Osthole, a natural coumarin derivative, has been reported to possess antioxidant and antiapoptotic activities. This study aimed to investigate the potential effects of osthole on renal I/R injury in an in vivo rat model. MATERIALS AND METHODS: We induced renal I/R injury by clamping the left renal artery for 45 min followed by reperfusion, along with a contralateral nephrectomy. We randomly assigned 54 rats to three groups (18 rats/group): sham-operated, vehicle-treated I/R, and osthole-treated I/R. We treated rats intraperitoneally with osthole (40 mg/kg) or vehicle (40 mg/kg) 30 min before renal ischemia. We harvested serum and kidneys at 1, 6, and 24 h after reperfusion. Renal function and histological changes were assessed. We also determined markers of oxidative stress and cell apoptosis in kidneys. RESULTS: Osthole treatment significantly attenuated renal dysfunction and histologic damage induced by I/R injury. The I/R-induced elevation in kidney malondialdehyde level decreased, whereas reduced kidney superoxide dismutase and catalase activities were markedly increased. Moreover, osthole-treated rats had a dramatic decrease in apoptotic tubular cells, along with a decrease in caspase-3 and an increase in the Bcl-2/Bax ratio. CONCLUSIONS: Osthole treatment protects murine kidney from renal I/R injury by suppressing oxidative stress and cell apoptosis. Thus, osthole may represent a novel practical strategy to prevent renal I/R injury. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/23391166/Osthole_ameliorates_renal_ischemia_reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(13)00009-7 DB - PRIME DP - Unbound Medicine ER -