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The venom of the fishing spider Dolomedes sulfurous contains various neurotoxins acting on voltage-activated ion channels in rat dorsal root ganglion neurons.
Toxicon. 2013 Apr; 65:68-75.T

Abstract

Dolomedes sulfurous is a venomous spider distributed in the south of China and characterized with feeding on fish. The venom exhibits great diversity and contains hundreds of peptides as revealed by off-line RP-HPLC/MALDI-TOF-MS analysis. The venom peptides followed a triple-modal distribution, with 40.7% of peptides falling in the mass range of 1000-3000 Da, 25.6% peptides in the 7000-9000 Da range and 23.5% peptides in the 3000-5000 Da range. This distribution modal is rather different from these of peptides from other spider venoms analyzed. The venom could inhibit voltage-activated Na(+), K(+) and Ca(2+) channels in rat DRG neurons as revealed by voltage-clamp analysis. Significantly, the venom exhibited inhibitory effects on TTX-R Na(+) and T-type Ca(2+) currents, suggesting that there exist both channel antagonists which might be valuable tools for investigation of both channels and drug development. Additionally, intrathoracically injection of venom could cause serve neurotoxic effects on zebrafish and death at higher concentrations. The LD50 value was calculated to be 28.8 μg/g body weight. Our results indicated that the venom of D. sulfurous contain diverse neurotoxins which serve to capture prey. Intensive studies will be necessary to investigate the structures and functions of specific peptides of the venom in the future.

Authors+Show Affiliations

Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23391637

Citation

Wang, Hengyun, et al. "The Venom of the Fishing Spider Dolomedes Sulfurous Contains Various Neurotoxins Acting On Voltage-activated Ion Channels in Rat Dorsal Root Ganglion Neurons." Toxicon : Official Journal of the International Society On Toxinology, vol. 65, 2013, pp. 68-75.
Wang H, Zhang F, Li D, et al. The venom of the fishing spider Dolomedes sulfurous contains various neurotoxins acting on voltage-activated ion channels in rat dorsal root ganglion neurons. Toxicon. 2013;65:68-75.
Wang, H., Zhang, F., Li, D., Xu, S., He, J., Yu, H., Li, J., Liu, Z., & Liang, S. (2013). The venom of the fishing spider Dolomedes sulfurous contains various neurotoxins acting on voltage-activated ion channels in rat dorsal root ganglion neurons. Toxicon : Official Journal of the International Society On Toxinology, 65, 68-75. https://doi.org/10.1016/j.toxicon.2013.01.014
Wang H, et al. The Venom of the Fishing Spider Dolomedes Sulfurous Contains Various Neurotoxins Acting On Voltage-activated Ion Channels in Rat Dorsal Root Ganglion Neurons. Toxicon. 2013;65:68-75. PubMed PMID: 23391637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The venom of the fishing spider Dolomedes sulfurous contains various neurotoxins acting on voltage-activated ion channels in rat dorsal root ganglion neurons. AU - Wang,Hengyun, AU - Zhang,Fan, AU - Li,Dan, AU - Xu,Shiyan, AU - He,Juan, AU - Yu,Hai, AU - Li,Jiayan, AU - Liu,Zhonghua, AU - Liang,Songping, Y1 - 2013/02/04/ PY - 2012/10/26/received PY - 2013/01/08/revised PY - 2013/01/16/accepted PY - 2013/2/9/entrez PY - 2013/2/9/pubmed PY - 2013/8/31/medline SP - 68 EP - 75 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 65 N2 - Dolomedes sulfurous is a venomous spider distributed in the south of China and characterized with feeding on fish. The venom exhibits great diversity and contains hundreds of peptides as revealed by off-line RP-HPLC/MALDI-TOF-MS analysis. The venom peptides followed a triple-modal distribution, with 40.7% of peptides falling in the mass range of 1000-3000 Da, 25.6% peptides in the 7000-9000 Da range and 23.5% peptides in the 3000-5000 Da range. This distribution modal is rather different from these of peptides from other spider venoms analyzed. The venom could inhibit voltage-activated Na(+), K(+) and Ca(2+) channels in rat DRG neurons as revealed by voltage-clamp analysis. Significantly, the venom exhibited inhibitory effects on TTX-R Na(+) and T-type Ca(2+) currents, suggesting that there exist both channel antagonists which might be valuable tools for investigation of both channels and drug development. Additionally, intrathoracically injection of venom could cause serve neurotoxic effects on zebrafish and death at higher concentrations. The LD50 value was calculated to be 28.8 μg/g body weight. Our results indicated that the venom of D. sulfurous contain diverse neurotoxins which serve to capture prey. Intensive studies will be necessary to investigate the structures and functions of specific peptides of the venom in the future. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/23391637/The_venom_of_the_fishing_spider_Dolomedes_sulfurous_contains_various_neurotoxins_acting_on_voltage_activated_ion_channels_in_rat_dorsal_root_ganglion_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(13)00040-8 DB - PRIME DP - Unbound Medicine ER -