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Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry.
Anal Bioanal Chem. 2013 Apr; 405(10):3331-40.AB

Abstract

Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable recoveries of P-CTXs (74-103 %) in fish blood. Matrix effects (6-26 %) in blood extracts were found to be significantly reduced compared with those in muscle extracts (suppressed by 34-75 % as reported in other studies), thereby minimizing potential for false negative results. The target P-CTXs were detectable in whole blood from four coral reef fish species collected in a CFP-endemic region. Similar trends in total P-CTX levels and patterns of P-CTX composition profiles in blood and muscle of these fish were observed, suggesting a relationship between blood and muscle levels of P-CTXs. This optimized method provides an essential tool for studies of P-CTX pharmacokinetics and pharmacodynamics in fish, which are needed for establishing the use of fish blood as a reliable sample for the assessment and control of CFP.

Authors+Show Affiliations

State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong SAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23392409

Citation

Mak, Yim Ling, et al. "Simultaneous Quantification of Pacific Ciguatoxins in Fish Blood Using Liquid Chromatography-tandem Mass Spectrometry." Analytical and Bioanalytical Chemistry, vol. 405, no. 10, 2013, pp. 3331-40.
Mak YL, Wu JJ, Chan WH, et al. Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2013;405(10):3331-40.
Mak, Y. L., Wu, J. J., Chan, W. H., Murphy, M. B., Lam, J. C., Chan, L. L., & Lam, P. K. (2013). Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 405(10), 3331-40. https://doi.org/10.1007/s00216-013-6766-8
Mak YL, et al. Simultaneous Quantification of Pacific Ciguatoxins in Fish Blood Using Liquid Chromatography-tandem Mass Spectrometry. Anal Bioanal Chem. 2013;405(10):3331-40. PubMed PMID: 23392409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry. AU - Mak,Yim Ling, AU - Wu,Jia Jun, AU - Chan,Wing Hei, AU - Murphy,Margaret B, AU - Lam,James C W, AU - Chan,Leo L, AU - Lam,Paul K S, Y1 - 2013/02/08/ PY - 2012/11/13/received PY - 2013/01/17/accepted PY - 2013/01/10/revised PY - 2013/2/9/entrez PY - 2013/2/9/pubmed PY - 2013/8/29/medline SP - 3331 EP - 40 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 405 IS - 10 N2 - Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable recoveries of P-CTXs (74-103 %) in fish blood. Matrix effects (6-26 %) in blood extracts were found to be significantly reduced compared with those in muscle extracts (suppressed by 34-75 % as reported in other studies), thereby minimizing potential for false negative results. The target P-CTXs were detectable in whole blood from four coral reef fish species collected in a CFP-endemic region. Similar trends in total P-CTX levels and patterns of P-CTX composition profiles in blood and muscle of these fish were observed, suggesting a relationship between blood and muscle levels of P-CTXs. This optimized method provides an essential tool for studies of P-CTX pharmacokinetics and pharmacodynamics in fish, which are needed for establishing the use of fish blood as a reliable sample for the assessment and control of CFP. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/23392409/Simultaneous_quantification_of_Pacific_ciguatoxins_in_fish_blood_using_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/s00216-013-6766-8 DB - PRIME DP - Unbound Medicine ER -