Tags

Type your tag names separated by a space and hit enter

Blocking of thromboxane A₂ receptor attenuates airway mucus hyperproduction induced by cigarette smoke.
Eur J Pharmacol. 2013 Mar 05; 703(1-3):11-7.EJ

Abstract

Cigarette smoking is one of the risk factors for chronic obstructive pulmonary disease (COPD). In this study, we investigated the effects of thromboxane A2 (TxA2) receptor antagonists on airway mucus production induced by cigarette smoke. Rats were exposed to cigarette smoke 1h/day, 6 days/week for 4 weeks. Seratrodast (2, 5, 10mg/kg day) was administered intragastrically prior to smoke exposure. Thromboxane B2 (TxB2) in the bronchoalveolar lavage fluid and lung tissues was determined by enzyme immunoassay. Airway mucus production was determined by alcin-blue/periodic acid sthiff (AB-PAS) staining, Muc5ac immunohistochemical staining, and RT-PCR. The phosphorylation of ERK and p38 was evaluated by Western blotting. Seratrodast reduced the overproduction of TxB2 in both bronchoalveolar lavage fluid and lung tissues. Cigarette smoke exposure markedly increased AB/PAS-stained goblet cells and rat Muc5ac expression in the airway, which was significantly attenuated by seratrodast administration. The induced phosphorylation of ERK and p38 was also attenuated by seratrodast. TxA2 receptor antagonist could reduce Muc5ac production induced by cigarette smoke in vivo, possibly through the mitogen-activated protein kinases (MAPK) signaling pathway.

Authors+Show Affiliations

Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23399768

Citation

An, Jing, et al. "Blocking of Thromboxane A₂ Receptor Attenuates Airway Mucus Hyperproduction Induced By Cigarette Smoke." European Journal of Pharmacology, vol. 703, no. 1-3, 2013, pp. 11-7.
An J, Li JQ, Wang T, et al. Blocking of thromboxane A₂ receptor attenuates airway mucus hyperproduction induced by cigarette smoke. Eur J Pharmacol. 2013;703(1-3):11-7.
An, J., Li, J. Q., Wang, T., Li, X. O., Guo, L. L., Wan, C., Liao, Z. L., Dong, J. J., Xu, D., & Wen, F. Q. (2013). Blocking of thromboxane A₂ receptor attenuates airway mucus hyperproduction induced by cigarette smoke. European Journal of Pharmacology, 703(1-3), 11-7. https://doi.org/10.1016/j.ejphar.2013.01.042
An J, et al. Blocking of Thromboxane A₂ Receptor Attenuates Airway Mucus Hyperproduction Induced By Cigarette Smoke. Eur J Pharmacol. 2013 Mar 5;703(1-3):11-7. PubMed PMID: 23399768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blocking of thromboxane A₂ receptor attenuates airway mucus hyperproduction induced by cigarette smoke. AU - An,Jing, AU - Li,Ji-Qiong, AU - Wang,Tao, AU - Li,Xiao-Ou, AU - Guo,Ling-Li, AU - Wan,Chun, AU - Liao,Zeng-Lin, AU - Dong,Jia-Jia, AU - Xu,Dan, AU - Wen,Fu-Qiang, Y1 - 2013/02/08/ PY - 2012/05/07/received PY - 2013/01/19/revised PY - 2013/01/29/accepted PY - 2013/2/13/entrez PY - 2013/2/13/pubmed PY - 2013/9/24/medline SP - 11 EP - 7 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 703 IS - 1-3 N2 - Cigarette smoking is one of the risk factors for chronic obstructive pulmonary disease (COPD). In this study, we investigated the effects of thromboxane A2 (TxA2) receptor antagonists on airway mucus production induced by cigarette smoke. Rats were exposed to cigarette smoke 1h/day, 6 days/week for 4 weeks. Seratrodast (2, 5, 10mg/kg day) was administered intragastrically prior to smoke exposure. Thromboxane B2 (TxB2) in the bronchoalveolar lavage fluid and lung tissues was determined by enzyme immunoassay. Airway mucus production was determined by alcin-blue/periodic acid sthiff (AB-PAS) staining, Muc5ac immunohistochemical staining, and RT-PCR. The phosphorylation of ERK and p38 was evaluated by Western blotting. Seratrodast reduced the overproduction of TxB2 in both bronchoalveolar lavage fluid and lung tissues. Cigarette smoke exposure markedly increased AB/PAS-stained goblet cells and rat Muc5ac expression in the airway, which was significantly attenuated by seratrodast administration. The induced phosphorylation of ERK and p38 was also attenuated by seratrodast. TxA2 receptor antagonist could reduce Muc5ac production induced by cigarette smoke in vivo, possibly through the mitogen-activated protein kinases (MAPK) signaling pathway. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/23399768/Blocking_of_thromboxane_A₂_receptor_attenuates_airway_mucus_hyperproduction_induced_by_cigarette_smoke_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(13)00062-9 DB - PRIME DP - Unbound Medicine ER -