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Cyclooxgenase-2 inhibiting perfluoropoly (ethylene glycol) ether theranostic nanoemulsions-in vitro study.
PLoS One. 2013; 8(2):e55802.Plos

Abstract

Cylcooxgenase-2 (COX-2) expressing macrophages, constituting a major portion of tumor mass, are involved in several pro-tumorigenic mechanisms. In addition, macrophages are actively recruited by the tumor and represent a viable target for anticancer therapy. COX-2 specific inhibitor, celecoxib, apart from its anticancer properties was shown to switch macrophage phenotype from tumor promoting to tumor suppressing. Celecoxib has low aqueous solubility, which may limit its tumor inhibiting effect. As opposed to oral administration, we propose that maximum anticancer effect may be achieved by nanoemulsion mediated intravenous delivery. Here we report multifunctional celecoxib nanoemulsions that can be imaged by both near-infrared fluorescence (NIRF) and (19)F magnetic resonance. Celecoxib loaded nanoemulsions showed a dose dependent uptake in mouse macrophages as measured by (19)F NMR and NIRF signal intensities of labeled cells. Dramatic inhibition of intracellular COX-2 enzyme was observed in activated macrophages upon nanoemulsion uptake. COX-2 enzyme inhibition was statistically equivalent between free drug and drug loaded nanoemulsion. However, nanoemulsion mediated drug delivery may be advantageous, helping to avoid systemic exposure to celecoxib and related side effects. Dual molecular imaging signatures of the presented nanoemulsions allow for future in vivo monitoring of the labeled macrophages and may help in examining the role of macrophage COX-2 inhibition in inflammation-cancer interactions. These features strongly support the future use of the presented nanoemulsions as anti-COX-2 theranostic nanomedicine with possible anticancer applications.

Authors+Show Affiliations

Graduate School of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University, Pittsburgh, Pennsylvania, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23409048

Citation

Patel, Sravan Kumar, et al. "Cyclooxgenase-2 Inhibiting Perfluoropoly (ethylene Glycol) Ether Theranostic Nanoemulsions-in Vitro Study." PloS One, vol. 8, no. 2, 2013, pp. e55802.
Patel SK, Zhang Y, Pollock JA, et al. Cyclooxgenase-2 inhibiting perfluoropoly (ethylene glycol) ether theranostic nanoemulsions-in vitro study. PLoS ONE. 2013;8(2):e55802.
Patel, S. K., Zhang, Y., Pollock, J. A., & Janjic, J. M. (2013). Cyclooxgenase-2 inhibiting perfluoropoly (ethylene glycol) ether theranostic nanoemulsions-in vitro study. PloS One, 8(2), e55802. https://doi.org/10.1371/journal.pone.0055802
Patel SK, et al. Cyclooxgenase-2 Inhibiting Perfluoropoly (ethylene Glycol) Ether Theranostic Nanoemulsions-in Vitro Study. PLoS ONE. 2013;8(2):e55802. PubMed PMID: 23409048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclooxgenase-2 inhibiting perfluoropoly (ethylene glycol) ether theranostic nanoemulsions-in vitro study. AU - Patel,Sravan Kumar, AU - Zhang,Yang, AU - Pollock,John A, AU - Janjic,Jelena M, Y1 - 2013/02/07/ PY - 2012/09/20/received PY - 2013/01/02/accepted PY - 2013/2/15/entrez PY - 2013/2/15/pubmed PY - 2013/8/6/medline SP - e55802 EP - e55802 JF - PloS one JO - PLoS ONE VL - 8 IS - 2 N2 - Cylcooxgenase-2 (COX-2) expressing macrophages, constituting a major portion of tumor mass, are involved in several pro-tumorigenic mechanisms. In addition, macrophages are actively recruited by the tumor and represent a viable target for anticancer therapy. COX-2 specific inhibitor, celecoxib, apart from its anticancer properties was shown to switch macrophage phenotype from tumor promoting to tumor suppressing. Celecoxib has low aqueous solubility, which may limit its tumor inhibiting effect. As opposed to oral administration, we propose that maximum anticancer effect may be achieved by nanoemulsion mediated intravenous delivery. Here we report multifunctional celecoxib nanoemulsions that can be imaged by both near-infrared fluorescence (NIRF) and (19)F magnetic resonance. Celecoxib loaded nanoemulsions showed a dose dependent uptake in mouse macrophages as measured by (19)F NMR and NIRF signal intensities of labeled cells. Dramatic inhibition of intracellular COX-2 enzyme was observed in activated macrophages upon nanoemulsion uptake. COX-2 enzyme inhibition was statistically equivalent between free drug and drug loaded nanoemulsion. However, nanoemulsion mediated drug delivery may be advantageous, helping to avoid systemic exposure to celecoxib and related side effects. Dual molecular imaging signatures of the presented nanoemulsions allow for future in vivo monitoring of the labeled macrophages and may help in examining the role of macrophage COX-2 inhibition in inflammation-cancer interactions. These features strongly support the future use of the presented nanoemulsions as anti-COX-2 theranostic nanomedicine with possible anticancer applications. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23409048/Cyclooxgenase_2_inhibiting_perfluoropoly__ethylene_glycol__ether_theranostic_nanoemulsions_in_vitro_study_ L2 - http://dx.plos.org/10.1371/journal.pone.0055802 DB - PRIME DP - Unbound Medicine ER -