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DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16.
Free Radic Res 2013; 47(4):316-24FR

Abstract

Earlier studies have demonstrated that Deuterohaemin-AlaHisThrValGluLys (DhHP-6), a novel porphyrin-peptide, increases lifespan and enhances stress resistance of Caenorhabditis elegans. To explore the possible mechanisms, in this study we investigated the roles of SIR-2.1 and DAF-16 in DhHP-6's function using wild-type and various other mutant strains of C. elegans. DhHP-6's effect was dependent upon DAF-16, and it did not extend the lifespan of the loss-of-function daf-16 mutant strain (daf-16(mu86) I). DhHP-6 enhanced DAF-16 translocation from cytoplasm to nuclei; and it increased DAF-16's transcriptional activity, likely by activating the SIR-2.1/DAF-16 complex. DhHP-6's effect was also dependent upon SIR-2.1, and it did not increase the lifespan of the worms with SIR-2.1 deacetylase activity inhibited by niacin amide (SIR-2.1 inhibitor) and SIR-2.1 RNA interference (RNAi). Niacin amide and RNAi increased DAF-16's nuclear localization; but they decreased DAF-16's transcriptional activity, likely by preventing the formation of the SIR-2.1/DAF-16 complex. These results suggest that DhHP-6 extends the lifespan of C. elegans via SIR 2.1 and DAF-16, and they provide new insights into the molecular mechanisms of aging.

Authors+Show Affiliations

College of Life Science, Jilin University, Changchun, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23410029

Citation

Huang, Lei, et al. "DhHP-6 Extends Lifespan of Caenorhabditis Elegans By Enhancing Nuclear Translocation and Transcriptional Activity of DAF-16." Free Radical Research, vol. 47, no. 4, 2013, pp. 316-24.
Huang L, Li P, Wang G, et al. DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16. Free Radic Res. 2013;47(4):316-24.
Huang, L., Li, P., Wang, G., Guan, S., Sun, X., & Wang, L. (2013). DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16. Free Radical Research, 47(4), pp. 316-24. doi:10.3109/10715762.2013.773588.
Huang L, et al. DhHP-6 Extends Lifespan of Caenorhabditis Elegans By Enhancing Nuclear Translocation and Transcriptional Activity of DAF-16. Free Radic Res. 2013;47(4):316-24. PubMed PMID: 23410029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16. AU - Huang,Lei, AU - Li,Pengfei, AU - Wang,Guan, AU - Guan,Shuwen, AU - Sun,Xiaoli, AU - Wang,Liping, Y1 - 2013/03/08/ PY - 2013/2/16/entrez PY - 2013/2/16/pubmed PY - 2013/9/5/medline SP - 316 EP - 24 JF - Free radical research JO - Free Radic. Res. VL - 47 IS - 4 N2 - Earlier studies have demonstrated that Deuterohaemin-AlaHisThrValGluLys (DhHP-6), a novel porphyrin-peptide, increases lifespan and enhances stress resistance of Caenorhabditis elegans. To explore the possible mechanisms, in this study we investigated the roles of SIR-2.1 and DAF-16 in DhHP-6's function using wild-type and various other mutant strains of C. elegans. DhHP-6's effect was dependent upon DAF-16, and it did not extend the lifespan of the loss-of-function daf-16 mutant strain (daf-16(mu86) I). DhHP-6 enhanced DAF-16 translocation from cytoplasm to nuclei; and it increased DAF-16's transcriptional activity, likely by activating the SIR-2.1/DAF-16 complex. DhHP-6's effect was also dependent upon SIR-2.1, and it did not increase the lifespan of the worms with SIR-2.1 deacetylase activity inhibited by niacin amide (SIR-2.1 inhibitor) and SIR-2.1 RNA interference (RNAi). Niacin amide and RNAi increased DAF-16's nuclear localization; but they decreased DAF-16's transcriptional activity, likely by preventing the formation of the SIR-2.1/DAF-16 complex. These results suggest that DhHP-6 extends the lifespan of C. elegans via SIR 2.1 and DAF-16, and they provide new insights into the molecular mechanisms of aging. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/23410029/DhHP_6_extends_lifespan_of_Caenorhabditis_elegans_by_enhancing_nuclear_translocation_and_transcriptional_activity_of_DAF_16_ L2 - http://www.tandfonline.com/doi/full/10.3109/10715762.2013.773588 DB - PRIME DP - Unbound Medicine ER -