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Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status.
Hum Reprod. 2013 Apr; 28(4):1054-61.HR

Abstract

STUDY QUESTION

Does DPY19L2 status influence intracytoplasmic sperm injection (ICSI) outcomes with or without assisted oocyte activation (AOA)?

SUMMARY ANSWER

DPY19L2 mutations have no major impact on ICSI outcomes in globozoospermic patients.

WHAT IS KNOWN ALREADY

Globozoospermia is a rare and severe teratozoospermia characterized by round-headed spermatozoa lacking an acrosome. Recently, it has been shown that DPY19L2 mutations can be found in a vast majority of, but not all, globozoospermic patients (66.7%). These patients suffer from primary infertility due to a sperm-related oocyte activation deficiency secondary to the absence of an acrosome that can be overcome by the application of AOA.

STUDY DESIGN, SIZE, DURATION

Cohort study, retrospective, 34 patients, 83 cycles.

MATERIALS, SETTING, METHODS

Clinical and biologic data were collected from 29 patients mutated for DPY19L2 and 5 non-mutated patients. In total, 35 ICSI cycles using AOA and 48 conventional ICSI cycles were included in the analysis. Patients were divided into groups according to whether or not they were mutated for DPY19L2 and whether or not they received AOA.

MAIN RESULTS AND THE ROLE OF CHANCE

Regardless of the presence of a DPY19L2 mutation, the fertilization rates with AOA are restored to normal when compared with conventional ICSI in our cohort of globozoospermic patients. Also, when performing ICSI plus AOA, both mutated and non-mutated cases have similar positive hCG rates, ongoing pregnancy rates and live birth rates per transfer. On the contrary, the fertilization rate in globozoospermic patients using conventional ICSI is correlated with the presence of a DPY19L2 mutation, with slightly better, although still very low, fertilization rates in patients carrying a DPY19L2 mutation. Nevertheless, when performing conventional ICSI, both mutated and non-mutated cases have similar very low positive hCG rates, ongoing pregnancy rates and live birth rates per transfer.

LIMITATIONS

A limitation of this study is the low number of included non-mutated cases.

WIDER IMPLICATIONS OF THE FINDINGS

We propose a pathway for the clinical management of globozoospermic patients depending on the phenotype that includes several diagnostic and therapeutic steps.

STUDY FUNDING/COMPETING INTEREST(S)

None.

Authors+Show Affiliations

Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut National de Santé et de Recherche Médicale (INSERM) U964, Centre National de Recherche Scientifique (CNRS) UMR1704, Université de Strasbourg, 67404 Illkirch, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23411621

Citation

Kuentz, P, et al. "Assisted Oocyte Activation Overcomes Fertilization Failure in Globozoospermic Patients Regardless of the DPY19L2 Status." Human Reproduction (Oxford, England), vol. 28, no. 4, 2013, pp. 1054-61.
Kuentz P, Vanden Meerschaut F, Elinati E, et al. Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status. Hum Reprod. 2013;28(4):1054-61.
Kuentz, P., Vanden Meerschaut, F., Elinati, E., Nasr-Esfahani, M. H., Gurgan, T., Iqbal, N., Carré-Pigeon, F., Brugnon, F., Gitlin, S. A., Velez de la Calle, J., Kilani, Z., De Sutter, P., & Viville, S. (2013). Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status. Human Reproduction (Oxford, England), 28(4), 1054-61. https://doi.org/10.1093/humrep/det005
Kuentz P, et al. Assisted Oocyte Activation Overcomes Fertilization Failure in Globozoospermic Patients Regardless of the DPY19L2 Status. Hum Reprod. 2013;28(4):1054-61. PubMed PMID: 23411621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status. AU - Kuentz,P, AU - Vanden Meerschaut,F, AU - Elinati,E, AU - Nasr-Esfahani,M H, AU - Gurgan,T, AU - Iqbal,N, AU - Carré-Pigeon,F, AU - Brugnon,F, AU - Gitlin,S A, AU - Velez de la Calle,J, AU - Kilani,Z, AU - De Sutter,P, AU - Viville,S, Y1 - 2013/02/14/ PY - 2013/2/16/entrez PY - 2013/2/16/pubmed PY - 2013/9/27/medline SP - 1054 EP - 61 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 28 IS - 4 N2 - STUDY QUESTION: Does DPY19L2 status influence intracytoplasmic sperm injection (ICSI) outcomes with or without assisted oocyte activation (AOA)? SUMMARY ANSWER: DPY19L2 mutations have no major impact on ICSI outcomes in globozoospermic patients. WHAT IS KNOWN ALREADY: Globozoospermia is a rare and severe teratozoospermia characterized by round-headed spermatozoa lacking an acrosome. Recently, it has been shown that DPY19L2 mutations can be found in a vast majority of, but not all, globozoospermic patients (66.7%). These patients suffer from primary infertility due to a sperm-related oocyte activation deficiency secondary to the absence of an acrosome that can be overcome by the application of AOA. STUDY DESIGN, SIZE, DURATION: Cohort study, retrospective, 34 patients, 83 cycles. MATERIALS, SETTING, METHODS: Clinical and biologic data were collected from 29 patients mutated for DPY19L2 and 5 non-mutated patients. In total, 35 ICSI cycles using AOA and 48 conventional ICSI cycles were included in the analysis. Patients were divided into groups according to whether or not they were mutated for DPY19L2 and whether or not they received AOA. MAIN RESULTS AND THE ROLE OF CHANCE: Regardless of the presence of a DPY19L2 mutation, the fertilization rates with AOA are restored to normal when compared with conventional ICSI in our cohort of globozoospermic patients. Also, when performing ICSI plus AOA, both mutated and non-mutated cases have similar positive hCG rates, ongoing pregnancy rates and live birth rates per transfer. On the contrary, the fertilization rate in globozoospermic patients using conventional ICSI is correlated with the presence of a DPY19L2 mutation, with slightly better, although still very low, fertilization rates in patients carrying a DPY19L2 mutation. Nevertheless, when performing conventional ICSI, both mutated and non-mutated cases have similar very low positive hCG rates, ongoing pregnancy rates and live birth rates per transfer. LIMITATIONS: A limitation of this study is the low number of included non-mutated cases. WIDER IMPLICATIONS OF THE FINDINGS: We propose a pathway for the clinical management of globozoospermic patients depending on the phenotype that includes several diagnostic and therapeutic steps. STUDY FUNDING/COMPETING INTEREST(S): None. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/23411621/Assisted_oocyte_activation_overcomes_fertilization_failure_in_globozoospermic_patients_regardless_of_the_DPY19L2_status_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/det005 DB - PRIME DP - Unbound Medicine ER -