Tags

Type your tag names separated by a space and hit enter

JNK and p38 MAPK regulate oxidative stress and the inflammatory response in chlorpyrifos-induced apoptosis.
Toxicol Lett. 2013 Apr 26; 218(3):235-45.TL

Abstract

To investigate mechanisms of neuronal cell death in response to chlorpyrifos (CPF), a pesticide, we evaluated the regulation of ROS and COX-2 in human neuroblastoma SH-SY5Y cells treated with CPF. CPF treatment produced cytotoxic effects that appeared to involve an increase in ROS. In addition, CPF treatment activated MAPK pathways including JNK, ERK1/2, and p38 MAPK, and MAPK inhibitors abolished the cytotoxicity and reduced ROS generation. Our data demonstrate that CPF induced apoptosis involving MAPK activation through ROS production. Furthermore, after the CPF treatment, COX-2 expression increased. Interestingly, JNK and p38 MAPK inhibitors attenuated the CPF-induced COX-2 expression while an ERK1/2 inhibitor did not. These findings suggest that pathways involving JNK and p38 MAPK, but not ERK1/2, mediated apoptosis and are involved in the inflammatory response. In conclusion, the JNK and p38 MAPK pathways might be critical mediators in CPF-induced neuronal apoptosis by both generating ROS and up-regulating COX-2.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Hanyang University, 133-791 Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23416140

Citation

Ki, Yeo-Woon, et al. "JNK and P38 MAPK Regulate Oxidative Stress and the Inflammatory Response in Chlorpyrifos-induced Apoptosis." Toxicology Letters, vol. 218, no. 3, 2013, pp. 235-45.
Ki YW, Park JH, Lee JE, et al. JNK and p38 MAPK regulate oxidative stress and the inflammatory response in chlorpyrifos-induced apoptosis. Toxicol Lett. 2013;218(3):235-45.
Ki, Y. W., Park, J. H., Lee, J. E., Shin, I. C., & Koh, H. C. (2013). JNK and p38 MAPK regulate oxidative stress and the inflammatory response in chlorpyrifos-induced apoptosis. Toxicology Letters, 218(3), 235-45. https://doi.org/10.1016/j.toxlet.2013.02.003
Ki YW, et al. JNK and P38 MAPK Regulate Oxidative Stress and the Inflammatory Response in Chlorpyrifos-induced Apoptosis. Toxicol Lett. 2013 Apr 26;218(3):235-45. PubMed PMID: 23416140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - JNK and p38 MAPK regulate oxidative stress and the inflammatory response in chlorpyrifos-induced apoptosis. AU - Ki,Yeo-Woon, AU - Park,Jae Hyeon, AU - Lee,Jeong Eun, AU - Shin,In Chul, AU - Koh,Hyun Chul, Y1 - 2013/02/14/ PY - 2012/11/23/received PY - 2013/01/29/revised PY - 2013/02/05/accepted PY - 2013/2/19/entrez PY - 2013/2/19/pubmed PY - 2013/5/17/medline SP - 235 EP - 45 JF - Toxicology letters JO - Toxicol Lett VL - 218 IS - 3 N2 - To investigate mechanisms of neuronal cell death in response to chlorpyrifos (CPF), a pesticide, we evaluated the regulation of ROS and COX-2 in human neuroblastoma SH-SY5Y cells treated with CPF. CPF treatment produced cytotoxic effects that appeared to involve an increase in ROS. In addition, CPF treatment activated MAPK pathways including JNK, ERK1/2, and p38 MAPK, and MAPK inhibitors abolished the cytotoxicity and reduced ROS generation. Our data demonstrate that CPF induced apoptosis involving MAPK activation through ROS production. Furthermore, after the CPF treatment, COX-2 expression increased. Interestingly, JNK and p38 MAPK inhibitors attenuated the CPF-induced COX-2 expression while an ERK1/2 inhibitor did not. These findings suggest that pathways involving JNK and p38 MAPK, but not ERK1/2, mediated apoptosis and are involved in the inflammatory response. In conclusion, the JNK and p38 MAPK pathways might be critical mediators in CPF-induced neuronal apoptosis by both generating ROS and up-regulating COX-2. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/23416140/JNK_and_p38_MAPK_regulate_oxidative_stress_and_the_inflammatory_response_in_chlorpyrifos_induced_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(13)00060-X DB - PRIME DP - Unbound Medicine ER -