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Alterations in inflammatory, antiviral and regulatory cytokine responses in peripheral blood mononuclear cells from pregnant women with asthma.
Respirology 2013; 18(5):827-33R

Abstract

BACKGROUND & OBJECTIVE

Severe asthma exacerbations during pregnancy are a common complication leading to poor health outcomes for both the mother and the baby. Asthma exacerbations are caused most frequently by respiratory viruses. A balance between antiviral and inflammatory immune responses is critical during pregnancy; the balance may be altered by asthma and respiratory virus infection.

METHODS

Peripheral blood mononuclear cells (PBMCs) were isolated from (i) non-pregnant healthy controls, (ii) pregnant non-asthmatics, (iii) post-partum non-asthmatics, (iv) non-pregnant asthmatics (v) pregnant asthmatics, and (vi) post-partum asthmatics. Cells were cultured in vitro with the mitogen phytohaemagglutinin or with a strain of the 2009 pandemic swine influenza. Interferon (IFN)-γ, interleukin (IL)-10 and IL-17 protein were measured from culture supernatant. Neutrophil counts were obtained in samples from pregnant and post-partum women.

RESULTS

Following the phytohaemagglutinin stimulation of PBMCs, pregnant asthmatics had significantly higher IL-17 and significantly lower IFN-γ responses compared with healthy non-pregnant women. Following infection with influenza, a significant reduction was also observed in IFN-γ and IL-10 production from PBMC of pregnant asthmatics. The IL-17 response to phytohaemagglutinin correlated with the neutrophil percentage. Differences in IFN-γ, IL-10 and IL-17 were found to persist for at least 6 months post-partum.

CONCLUSIONS

A reduction in antiviral and regulatory immunity with increased inflammation during pregnancy occurs in PBMC from pregnant women with asthma. This novel information may relate to the increased susceptibility and disease severity to respiratory virus infections observed during pregnancy.

Authors+Show Affiliations

Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle, Newcastle, New South Wales, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23421904

Citation

Vanders, Rebecca L., et al. "Alterations in Inflammatory, Antiviral and Regulatory Cytokine Responses in Peripheral Blood Mononuclear Cells From Pregnant Women With Asthma." Respirology (Carlton, Vic.), vol. 18, no. 5, 2013, pp. 827-33.
Vanders RL, Gibson PG, Wark PA, et al. Alterations in inflammatory, antiviral and regulatory cytokine responses in peripheral blood mononuclear cells from pregnant women with asthma. Respirology. 2013;18(5):827-33.
Vanders, R. L., Gibson, P. G., Wark, P. A., & Murphy, V. E. (2013). Alterations in inflammatory, antiviral and regulatory cytokine responses in peripheral blood mononuclear cells from pregnant women with asthma. Respirology (Carlton, Vic.), 18(5), pp. 827-33. doi:10.1111/resp.12068.
Vanders RL, et al. Alterations in Inflammatory, Antiviral and Regulatory Cytokine Responses in Peripheral Blood Mononuclear Cells From Pregnant Women With Asthma. Respirology. 2013;18(5):827-33. PubMed PMID: 23421904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations in inflammatory, antiviral and regulatory cytokine responses in peripheral blood mononuclear cells from pregnant women with asthma. AU - Vanders,Rebecca L, AU - Gibson,Peter G, AU - Wark,Peter A B, AU - Murphy,Vanessa E, PY - 2012/09/17/received PY - 2012/11/29/revised PY - 2012/12/27/accepted PY - 2013/2/21/entrez PY - 2013/2/21/pubmed PY - 2014/3/13/medline SP - 827 EP - 33 JF - Respirology (Carlton, Vic.) JO - Respirology VL - 18 IS - 5 N2 - BACKGROUND & OBJECTIVE: Severe asthma exacerbations during pregnancy are a common complication leading to poor health outcomes for both the mother and the baby. Asthma exacerbations are caused most frequently by respiratory viruses. A balance between antiviral and inflammatory immune responses is critical during pregnancy; the balance may be altered by asthma and respiratory virus infection. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from (i) non-pregnant healthy controls, (ii) pregnant non-asthmatics, (iii) post-partum non-asthmatics, (iv) non-pregnant asthmatics (v) pregnant asthmatics, and (vi) post-partum asthmatics. Cells were cultured in vitro with the mitogen phytohaemagglutinin or with a strain of the 2009 pandemic swine influenza. Interferon (IFN)-γ, interleukin (IL)-10 and IL-17 protein were measured from culture supernatant. Neutrophil counts were obtained in samples from pregnant and post-partum women. RESULTS: Following the phytohaemagglutinin stimulation of PBMCs, pregnant asthmatics had significantly higher IL-17 and significantly lower IFN-γ responses compared with healthy non-pregnant women. Following infection with influenza, a significant reduction was also observed in IFN-γ and IL-10 production from PBMC of pregnant asthmatics. The IL-17 response to phytohaemagglutinin correlated with the neutrophil percentage. Differences in IFN-γ, IL-10 and IL-17 were found to persist for at least 6 months post-partum. CONCLUSIONS: A reduction in antiviral and regulatory immunity with increased inflammation during pregnancy occurs in PBMC from pregnant women with asthma. This novel information may relate to the increased susceptibility and disease severity to respiratory virus infections observed during pregnancy. SN - 1440-1843 UR - https://www.unboundmedicine.com/medline/citation/23421904/Alterations_in_inflammatory_antiviral_and_regulatory_cytokine_responses_in_peripheral_blood_mononuclear_cells_from_pregnant_women_with_asthma_ L2 - https://doi.org/10.1111/resp.12068 DB - PRIME DP - Unbound Medicine ER -