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Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection.
J Chromatogr A. 2013 Mar 22; 1282:161-6.JC

Abstract

A noncompetitive immunoassay based on gold nanoparticles (AuNPs) amplified capillary electrophoresis (CE) chemiluminescence (CL) detection was developed for the determination of carcinoembryonic antigen (CEA). In this method, citrate-modified AuNPs were conjugated with horseradish peroxidase (HRP) labeled CEA antibody (Ab*), and incubated with limited amount of CEA antigen. CEA-Ab*-AuNPs complex and excess of Ab*-AuNPs were then separated and quantified by CE with CL detection. Highly sensitive CL detection was achieved by means of p-iodophenol (PIP) enhanced luminol-H2O2-HPR CL reaction and AuNPs amplified. Under the optimal conditions, the CE assay was accomplished within 5min. The linear range for CEA detection was 0.05-20ng/mL with a detection limit of 0.034ng/mL (signal/noise=3), which is three orders magnitude lower than that of without AuNPs amplified. The current method was successfully applied for the quantification of CEA in human serum samples. It was demonstrated that the current CE-CL AuNPs amplified noncompetitive immunoassay was sensitive and highly selective. It may serve as a tool for clinical analysis of CEA to assist in the diagnosis of cancer.

Authors+Show Affiliations

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23422894

Citation

Jiang, Jing, et al. "Highly Sensitive Immunoassay of Carcinoembryonic Antigen By Capillary Electrophoresis With Gold Nanoparticles Amplified Chemiluminescence Detection." Journal of Chromatography. A, vol. 1282, 2013, pp. 161-6.
Jiang J, Zhao S, Huang Y, et al. Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection. J Chromatogr A. 2013;1282:161-6.
Jiang, J., Zhao, S., Huang, Y., Qin, G., & Ye, F. (2013). Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection. Journal of Chromatography. A, 1282, 161-6. https://doi.org/10.1016/j.chroma.2013.01.066
Jiang J, et al. Highly Sensitive Immunoassay of Carcinoembryonic Antigen By Capillary Electrophoresis With Gold Nanoparticles Amplified Chemiluminescence Detection. J Chromatogr A. 2013 Mar 22;1282:161-6. PubMed PMID: 23422894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection. AU - Jiang,Jing, AU - Zhao,Shulin, AU - Huang,Yong, AU - Qin,Guoxin, AU - Ye,Fanggui, Y1 - 2013/01/23/ PY - 2012/10/04/received PY - 2013/01/14/revised PY - 2013/01/15/accepted PY - 2013/2/21/entrez PY - 2013/2/21/pubmed PY - 2013/5/15/medline SP - 161 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1282 N2 - A noncompetitive immunoassay based on gold nanoparticles (AuNPs) amplified capillary electrophoresis (CE) chemiluminescence (CL) detection was developed for the determination of carcinoembryonic antigen (CEA). In this method, citrate-modified AuNPs were conjugated with horseradish peroxidase (HRP) labeled CEA antibody (Ab*), and incubated with limited amount of CEA antigen. CEA-Ab*-AuNPs complex and excess of Ab*-AuNPs were then separated and quantified by CE with CL detection. Highly sensitive CL detection was achieved by means of p-iodophenol (PIP) enhanced luminol-H2O2-HPR CL reaction and AuNPs amplified. Under the optimal conditions, the CE assay was accomplished within 5min. The linear range for CEA detection was 0.05-20ng/mL with a detection limit of 0.034ng/mL (signal/noise=3), which is three orders magnitude lower than that of without AuNPs amplified. The current method was successfully applied for the quantification of CEA in human serum samples. It was demonstrated that the current CE-CL AuNPs amplified noncompetitive immunoassay was sensitive and highly selective. It may serve as a tool for clinical analysis of CEA to assist in the diagnosis of cancer. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/23422894/Highly_sensitive_immunoassay_of_carcinoembryonic_antigen_by_capillary_electrophoresis_with_gold_nanoparticles_amplified_chemiluminescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(13)00170-2 DB - PRIME DP - Unbound Medicine ER -