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[Ovariectomy and calcium/vitamin D2/D3 deficient diet as a model of osteoporosis in the spine of Sprague-Dawley rats].
Z Orthop Unfall. 2013 Feb; 151(1):14-9.ZO

Abstract

BACKGROUND

Osteoporosis is a widespread disease characterised by low bone mass and structural deterioration of bone resulting in an increased susceptibility to fractures. Osteoporosis affects women more frequently than men; every second woman older than 50 years suffers from an osteoporotic fracture, frequently a vertebral fracture. The aim of this study was to induce osteoporosis in rats to establish an osteoporotic small-animal model that simulates the human pathology particularly in the spine. Therefore, bone density parameters, which are routinely determined in the spine of osteoporotic patients, were investigated by Dual-Energy X-ray Absorptiometry (DEXA).

MATERIALS AND METHODS

Fourteen-week-old female Sprague-Dawley rats (n = 50) were either sham-operated (control group: sham) or ovariectomised (experimental group). Ovariectomised rats were further divided into two groups; one received calcium/vitamin D2/D3 deficient diet (OVX + diet), and the other received subcutaneous steroid injections (dexamethasone 0.3 mg/kg body weight) twice a month (OVX + steroid). Rats were scanned by DEXA at three time points (Month = M, 0 M, 1 M and 3 M). DEXA measurement of the spine delivered T-value, Z-value, bone mineral content (BMC), and the scanned area. Fifteen female patients at an age of 57-72 years were scanned in 8-10 regions of the spine (150 measurements). T-values and Z-values were pre-calculated based on patient databases. Statistical analysis was performed using two-way ANOVA followed by Bonferroni correction, with significance considered at p < 0.05.

RESULTS

T-value and Z-value of both rat groups were compared with the patient data as well as with each others. Both treated rat groups revealed significantly lower T- and Z-values than controls. Despite the significant difference, the reference line (-2.5 for T-value and -1.5 for Z-value) was only reached by the OVX + diet group. On the other hand, the sham group showed an increase in BMC over time, while no change was seen in OVX + diet or OVX + steroid. Bone area demonstrated a significant increase up to M3. However, the increase in bone area within the OVX + diet group was notably higher than in both sham and OVX + steroid groups. Patients showed significantly lower T- and Z-values than sham and OVX + steroid but insignificant ones when compared with OVX + diet.

CONCLUSION

A reproducible vertebral osteoporosis can be generated in a rat model by combination of ovariectomy with administration of a calcium/vitamin D3 deficient diet. T- and Z-values of this experimental group mimicked values obtained from osteoporotic patients, reflecting a simulation of their pathology. Interestingly, the increase in bone area over time with the steady BMC results in lower mineral density (BMD) of the OVX + diet group. Therefore, this rat model presents a reliable experimental set-up that may serve as a tool to better understand and treat osteoporosis.

Authors+Show Affiliations

Klinik und Poliklinik für Unfallchirurgie, Universitätsklinikum Gieβen und Marburg GmbH, Gieβen. gudrun.schlewitz@chiru.med.uni-giessen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

ger

PubMed ID

23423586

Citation

Schlewitz, G, et al. "[Ovariectomy and Calcium/vitamin D2/D3 Deficient Diet as a Model of Osteoporosis in the Spine of Sprague-Dawley Rats]." Zeitschrift Fur Orthopadie Und Unfallchirurgie, vol. 151, no. 1, 2013, pp. 14-9.
Schlewitz G, Govindarajan P, Schliefke N, et al. [Ovariectomy and calcium/vitamin D2/D3 deficient diet as a model of osteoporosis in the spine of Sprague-Dawley rats]. Z Orthop Unfall. 2013;151(1):14-9.
Schlewitz, G., Govindarajan, P., Schliefke, N., Alt, V., Böcker, W., Elkhassawna, T., Thormann, U., Lips, K. S., Hemdan, N. Y., Zahner, D., Schnettler, R., & Heiss, C. (2013). [Ovariectomy and calcium/vitamin D2/D3 deficient diet as a model of osteoporosis in the spine of Sprague-Dawley rats]. Zeitschrift Fur Orthopadie Und Unfallchirurgie, 151(1), 14-9. https://doi.org/10.1055/s-0032-1327976
Schlewitz G, et al. [Ovariectomy and Calcium/vitamin D2/D3 Deficient Diet as a Model of Osteoporosis in the Spine of Sprague-Dawley Rats]. Z Orthop Unfall. 2013;151(1):14-9. PubMed PMID: 23423586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Ovariectomy and calcium/vitamin D2/D3 deficient diet as a model of osteoporosis in the spine of Sprague-Dawley rats]. AU - Schlewitz,G, AU - Govindarajan,P, AU - Schliefke,N, AU - Alt,V, AU - Böcker,W, AU - Elkhassawna,T, AU - Thormann,U, AU - Lips,K S, AU - Hemdan,N Y, AU - Zahner,D, AU - Schnettler,R, AU - Heiss,C, Y1 - 2013/02/19/ PY - 2013/2/21/entrez PY - 2013/2/21/pubmed PY - 2013/8/27/medline SP - 14 EP - 9 JF - Zeitschrift fur Orthopadie und Unfallchirurgie JO - Z Orthop Unfall VL - 151 IS - 1 N2 - BACKGROUND: Osteoporosis is a widespread disease characterised by low bone mass and structural deterioration of bone resulting in an increased susceptibility to fractures. Osteoporosis affects women more frequently than men; every second woman older than 50 years suffers from an osteoporotic fracture, frequently a vertebral fracture. The aim of this study was to induce osteoporosis in rats to establish an osteoporotic small-animal model that simulates the human pathology particularly in the spine. Therefore, bone density parameters, which are routinely determined in the spine of osteoporotic patients, were investigated by Dual-Energy X-ray Absorptiometry (DEXA). MATERIALS AND METHODS: Fourteen-week-old female Sprague-Dawley rats (n = 50) were either sham-operated (control group: sham) or ovariectomised (experimental group). Ovariectomised rats were further divided into two groups; one received calcium/vitamin D2/D3 deficient diet (OVX + diet), and the other received subcutaneous steroid injections (dexamethasone 0.3 mg/kg body weight) twice a month (OVX + steroid). Rats were scanned by DEXA at three time points (Month = M, 0 M, 1 M and 3 M). DEXA measurement of the spine delivered T-value, Z-value, bone mineral content (BMC), and the scanned area. Fifteen female patients at an age of 57-72 years were scanned in 8-10 regions of the spine (150 measurements). T-values and Z-values were pre-calculated based on patient databases. Statistical analysis was performed using two-way ANOVA followed by Bonferroni correction, with significance considered at p < 0.05. RESULTS: T-value and Z-value of both rat groups were compared with the patient data as well as with each others. Both treated rat groups revealed significantly lower T- and Z-values than controls. Despite the significant difference, the reference line (-2.5 for T-value and -1.5 for Z-value) was only reached by the OVX + diet group. On the other hand, the sham group showed an increase in BMC over time, while no change was seen in OVX + diet or OVX + steroid. Bone area demonstrated a significant increase up to M3. However, the increase in bone area within the OVX + diet group was notably higher than in both sham and OVX + steroid groups. Patients showed significantly lower T- and Z-values than sham and OVX + steroid but insignificant ones when compared with OVX + diet. CONCLUSION: A reproducible vertebral osteoporosis can be generated in a rat model by combination of ovariectomy with administration of a calcium/vitamin D3 deficient diet. T- and Z-values of this experimental group mimicked values obtained from osteoporotic patients, reflecting a simulation of their pathology. Interestingly, the increase in bone area over time with the steady BMC results in lower mineral density (BMD) of the OVX + diet group. Therefore, this rat model presents a reliable experimental set-up that may serve as a tool to better understand and treat osteoporosis. SN - 1864-6743 UR - https://www.unboundmedicine.com/medline/citation/23423586/[Ovariectomy_and_calcium/vitamin_D2/D3_deficient_diet_as_a_model_of_osteoporosis_in_the_spine_of_Sprague_Dawley_rats]_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0032-1327976 DB - PRIME DP - Unbound Medicine ER -