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Antioxidant capacities, phenolic contents, and GC/MS analysis of Rhodiola imbricata Edgew. root extracts from Trans-Himalaya.
J Food Sci. 2013 Mar; 78(3):C402-10.JF

Abstract

Our aim was to assess the antioxidant capacities and phenolic constituents of methanol and aqueous extracts of Rhodiola imbricata Edgew. root from Trans-Himalayan cold desert of Ladakh. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacity of the root extracts increased in a dose-dependent manner (up to 0.1 mg/mL) and root extract concentrations required for 50% inhibition of radical scavenging effect (IC50) were recorded as 0.013 and 0.014 mg/mL (for DPPH) and 0.016 and 0.017 mg/mL (for ABTS) for methanol and aqueous extracts, respectively. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total polyphenol and phenolic acid content of methanol and aqueous extracts were 112.24, 59.06, 39.02, and 16.95 mg gallic acid equivalent/g of extract, respectively. Total flavonoid and flavonol contents were estimated to be 30.2, 17.67, 20.68, and 7.38 mg quercetin equivalent/g of extract, respectively. In all antioxidant capacity assays, the methanol extract exhibited significantly higher antioxidant capacity than that of aqueous extract due to the presence of significantly higher amount of vital phytoconstitiuents, viz. polyphenol, phenolic acid, and flavonol. GC/MS analysis showed that phytosterols, alkyl halide, phenols, and fatty acid esters were major phytochemical clusters. On the other hand, monoterpenes, fatty acids, tocopherols, aliphatic hydrocarbons, and ethers were found to be present in comparatively less amount in the methanol extract. Hence, our study signifies that this high-altitude medicinal herb could be used as the natural source of antioxidants and supports its use in traditional system of medicine to ameliorate oxidative stress and high-altitude maladies.

Authors+Show Affiliations

Defence Institute of High Altitude Research, Defence Research & Development Organisation, Leh-Ladakh, Jammu & Kashmir, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23425091

Citation

Tayade, A B., et al. "Antioxidant Capacities, Phenolic Contents, and GC/MS Analysis of Rhodiola Imbricata Edgew. Root Extracts From Trans-Himalaya." Journal of Food Science, vol. 78, no. 3, 2013, pp. C402-10.
Tayade AB, Dhar P, Sharma M, et al. Antioxidant capacities, phenolic contents, and GC/MS analysis of Rhodiola imbricata Edgew. root extracts from Trans-Himalaya. J Food Sci. 2013;78(3):C402-10.
Tayade, A. B., Dhar, P., Sharma, M., Chauhan, R. S., Chaurasia, O. P., & Srivastava, R. B. (2013). Antioxidant capacities, phenolic contents, and GC/MS analysis of Rhodiola imbricata Edgew. root extracts from Trans-Himalaya. Journal of Food Science, 78(3), C402-10. https://doi.org/10.1111/1750-3841.12054
Tayade AB, et al. Antioxidant Capacities, Phenolic Contents, and GC/MS Analysis of Rhodiola Imbricata Edgew. Root Extracts From Trans-Himalaya. J Food Sci. 2013;78(3):C402-10. PubMed PMID: 23425091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant capacities, phenolic contents, and GC/MS analysis of Rhodiola imbricata Edgew. root extracts from Trans-Himalaya. AU - Tayade,A B, AU - Dhar,P, AU - Sharma,M, AU - Chauhan,R S, AU - Chaurasia,O P, AU - Srivastava,R B, Y1 - 2013/02/20/ PY - 2012/07/10/received PY - 2012/12/03/accepted PY - 2013/2/22/entrez PY - 2013/2/22/pubmed PY - 2013/9/21/medline SP - C402 EP - 10 JF - Journal of food science JO - J Food Sci VL - 78 IS - 3 N2 - Our aim was to assess the antioxidant capacities and phenolic constituents of methanol and aqueous extracts of Rhodiola imbricata Edgew. root from Trans-Himalayan cold desert of Ladakh. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacity of the root extracts increased in a dose-dependent manner (up to 0.1 mg/mL) and root extract concentrations required for 50% inhibition of radical scavenging effect (IC50) were recorded as 0.013 and 0.014 mg/mL (for DPPH) and 0.016 and 0.017 mg/mL (for ABTS) for methanol and aqueous extracts, respectively. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total polyphenol and phenolic acid content of methanol and aqueous extracts were 112.24, 59.06, 39.02, and 16.95 mg gallic acid equivalent/g of extract, respectively. Total flavonoid and flavonol contents were estimated to be 30.2, 17.67, 20.68, and 7.38 mg quercetin equivalent/g of extract, respectively. In all antioxidant capacity assays, the methanol extract exhibited significantly higher antioxidant capacity than that of aqueous extract due to the presence of significantly higher amount of vital phytoconstitiuents, viz. polyphenol, phenolic acid, and flavonol. GC/MS analysis showed that phytosterols, alkyl halide, phenols, and fatty acid esters were major phytochemical clusters. On the other hand, monoterpenes, fatty acids, tocopherols, aliphatic hydrocarbons, and ethers were found to be present in comparatively less amount in the methanol extract. Hence, our study signifies that this high-altitude medicinal herb could be used as the natural source of antioxidants and supports its use in traditional system of medicine to ameliorate oxidative stress and high-altitude maladies. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/23425091/Antioxidant_capacities_phenolic_contents_and_GC/MS_analysis_of_Rhodiola_imbricata_Edgew__root_extracts_from_Trans_Himalaya_ L2 - https://doi.org/10.1111/1750-3841.12054 DB - PRIME DP - Unbound Medicine ER -