Tags

Type your tag names separated by a space and hit enter

Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism.
J Virol. 2013 Jun; 87(11):6081-90.JV

Abstract

The human airway epithelium (HAE) represents the entry port of many human respiratory viruses, including human coronaviruses (HCoVs). Nowadays, four HCoVs, HCoV-229E, HCoV-OC43, HCoV-HKU1, and HCoV-NL63, are known to be circulating worldwide, causing upper and lower respiratory tract infections in nonhospitalized and hospitalized children. Studies of the fundamental aspects of these HCoV infections at the primary entry port, such as cell tropism, are seriously hampered by the lack of a universal culture system or suitable animal models. To expand the knowledge on fundamental virus-host interactions for all four HCoVs at the site of primary infection, we used pseudostratified HAE cell cultures to isolate and characterize representative clinical HCoV strains directly from nasopharyngeal material. Ten contemporary isolates were obtained, representing HCoV-229E (n = 1), HCoV-NL63 (n = 1), HCoV-HKU1 (n = 4), and HCoV-OC43 (n = 4). For each strain, we analyzed the replication kinetics and progeny virus release on HAE cell cultures derived from different donors. Surprisingly, by visualizing HCoV infection by confocal microscopy, we observed that HCoV-229E employs a target cell tropism for nonciliated cells, whereas HCoV-OC43, HCoV-HKU1, and HCoV-NL63 all infect ciliated cells. Collectively, the data demonstrate that HAE cell cultures, which morphologically and functionally resemble human airways in vivo, represent a robust universal culture system for isolating and comparing all contemporary HCoV strains.

Authors+Show Affiliations

Institute of Immunobiology, Kantonal Hospital, St. Gallen, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23427150

Citation

Dijkman, Ronald, et al. "Isolation and Characterization of Current Human Coronavirus Strains in Primary Human Epithelial Cell Cultures Reveal Differences in Target Cell Tropism." Journal of Virology, vol. 87, no. 11, 2013, pp. 6081-90.
Dijkman R, Jebbink MF, Koekkoek SM, et al. Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism. J Virol. 2013;87(11):6081-90.
Dijkman, R., Jebbink, M. F., Koekkoek, S. M., Deijs, M., Jónsdóttir, H. R., Molenkamp, R., Ieven, M., Goossens, H., Thiel, V., & van der Hoek, L. (2013). Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism. Journal of Virology, 87(11), 6081-90. https://doi.org/10.1128/JVI.03368-12
Dijkman R, et al. Isolation and Characterization of Current Human Coronavirus Strains in Primary Human Epithelial Cell Cultures Reveal Differences in Target Cell Tropism. J Virol. 2013;87(11):6081-90. PubMed PMID: 23427150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism. AU - Dijkman,Ronald, AU - Jebbink,Maarten F, AU - Koekkoek,Sylvie M, AU - Deijs,Martin, AU - Jónsdóttir,Hulda R, AU - Molenkamp,Richard, AU - Ieven,Margareta, AU - Goossens,Herman, AU - Thiel,Volker, AU - van der Hoek,Lia, Y1 - 2013/02/20/ PY - 2013/2/22/entrez PY - 2013/2/22/pubmed PY - 2013/7/31/medline SP - 6081 EP - 90 JF - Journal of virology JO - J. Virol. VL - 87 IS - 11 N2 - The human airway epithelium (HAE) represents the entry port of many human respiratory viruses, including human coronaviruses (HCoVs). Nowadays, four HCoVs, HCoV-229E, HCoV-OC43, HCoV-HKU1, and HCoV-NL63, are known to be circulating worldwide, causing upper and lower respiratory tract infections in nonhospitalized and hospitalized children. Studies of the fundamental aspects of these HCoV infections at the primary entry port, such as cell tropism, are seriously hampered by the lack of a universal culture system or suitable animal models. To expand the knowledge on fundamental virus-host interactions for all four HCoVs at the site of primary infection, we used pseudostratified HAE cell cultures to isolate and characterize representative clinical HCoV strains directly from nasopharyngeal material. Ten contemporary isolates were obtained, representing HCoV-229E (n = 1), HCoV-NL63 (n = 1), HCoV-HKU1 (n = 4), and HCoV-OC43 (n = 4). For each strain, we analyzed the replication kinetics and progeny virus release on HAE cell cultures derived from different donors. Surprisingly, by visualizing HCoV infection by confocal microscopy, we observed that HCoV-229E employs a target cell tropism for nonciliated cells, whereas HCoV-OC43, HCoV-HKU1, and HCoV-NL63 all infect ciliated cells. Collectively, the data demonstrate that HAE cell cultures, which morphologically and functionally resemble human airways in vivo, represent a robust universal culture system for isolating and comparing all contemporary HCoV strains. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/23427150/Isolation_and_characterization_of_current_human_coronavirus_strains_in_primary_human_epithelial_cell_cultures_reveal_differences_in_target_cell_tropism_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=23427150 DB - PRIME DP - Unbound Medicine ER -