Tags

Type your tag names separated by a space and hit enter

Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon.
Tsitol Genet. 2013 Jan-Feb; 47(1):3-10.TG

Abstract

Thinopyrum intermedium is a useful source of resistance genes for Barley Yellow Dwarf Virus (BYDV), one of the most damaging wheat diseases. In this study, wheat/Th. intermedium translocation lines with a BYDV resistance gene were developed using the Th. intermedium 7Ai- 1 chromosome. Genomic in situ hybridization (GISH), using a Th. intermedium total genomic DNA probe, enabled detection of 7Ai-1-derived small chromatins containing a BYDV resistance gene, which were translocated onto the end of wheat chromosomes in the lines Y95011 and Y960843. Random amplified polymorphic DNA (RAPD) analyses using 120 random 10-mer primers were conducted to compare the BYDV-resistant translocation lines with susceptible lines. Two primers amplified the DNA fragments specific to the resistant line that would be useful as molecular markers to identify 7Ai-1-derived BYDV resistance chromatin in the wheat genome. Additionally, the isolated Th. intermedium-specific retrotransposon-like sequence pTi28 can be used to identify Th. intermedium chromatin transferred to the wheat genome.

Authors+Show Affiliations

Molecular Genetics Laboratory, Faculty of Agriculture Tottori University, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23427606

Citation

Ma, Y-Z, and M Tomita. "Thinopyrum 7Ai-1-derived Small Chromatin With Barley Yellow Dwarf Virus (BYDV) Resistance Gene Integrated Into the Wheat Genome With Retrotransposon." TSitologiia I Genetika, vol. 47, no. 1, 2013, pp. 3-10.
Ma YZ, Tomita M. Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon. Tsitol Genet. 2013;47(1):3-10.
Ma, Y. Z., & Tomita, M. (2013). Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon. TSitologiia I Genetika, 47(1), 3-10.
Ma YZ, Tomita M. Thinopyrum 7Ai-1-derived Small Chromatin With Barley Yellow Dwarf Virus (BYDV) Resistance Gene Integrated Into the Wheat Genome With Retrotransposon. Tsitol Genet. 2013 Jan-Feb;47(1):3-10. PubMed PMID: 23427606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon. AU - Ma,Y-Z, AU - Tomita,M, PY - 2013/2/23/entrez PY - 2013/2/23/pubmed PY - 2013/6/29/medline SP - 3 EP - 10 JF - TSitologiia i genetika JO - Tsitol Genet VL - 47 IS - 1 N2 - Thinopyrum intermedium is a useful source of resistance genes for Barley Yellow Dwarf Virus (BYDV), one of the most damaging wheat diseases. In this study, wheat/Th. intermedium translocation lines with a BYDV resistance gene were developed using the Th. intermedium 7Ai- 1 chromosome. Genomic in situ hybridization (GISH), using a Th. intermedium total genomic DNA probe, enabled detection of 7Ai-1-derived small chromatins containing a BYDV resistance gene, which were translocated onto the end of wheat chromosomes in the lines Y95011 and Y960843. Random amplified polymorphic DNA (RAPD) analyses using 120 random 10-mer primers were conducted to compare the BYDV-resistant translocation lines with susceptible lines. Two primers amplified the DNA fragments specific to the resistant line that would be useful as molecular markers to identify 7Ai-1-derived BYDV resistance chromatin in the wheat genome. Additionally, the isolated Th. intermedium-specific retrotransposon-like sequence pTi28 can be used to identify Th. intermedium chromatin transferred to the wheat genome. SN - 0564-3783 UR - https://www.unboundmedicine.com/medline/citation/23427606/Thinopyrum_7Ai_1_derived_small_chromatin_with_Barley_Yellow_Dwarf_Virus__BYDV__resistance_gene_integrated_into_the_wheat_genome_with_retrotransposon_ DB - PRIME DP - Unbound Medicine ER -