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Native fluorescence detection of biomolecular and pharmaceutical compounds in capillary electrophoresis: detector designs, performance and applications: a review.
Anal Chim Acta. 2013 Mar 05; 766:13-33.AC

Abstract

This review treats the coupling of capillary electrophoresis (CE) with fluorescence detection (Flu) for the analysis of natively fluorescent biomolecular and pharmaceutical compounds. CE-Flu combines the excellent separation efficiency of CE with the high selectivity and sensitivity of Flu. In CE-Flu, an appropriate design of the fluorescence detection cell is very important in order to achieve efficient analyte excitation in and emission light collection from the small cylindrically-shaped detection volume. Therefore, due attention is paid to the various optical detection designs used for CE-Flu, including the applied excitation sources and emission light detectors. Special attention is devoted to wavelength-resolved Flu and to sensitivity issues. Furthermore, he specific requirements for fluorescence detection in microfluidic systems (i.e. chip-based electrophoresis) are discussed. Subsequently, an overview of described applications of CE-Flu for the analysis of natively fluorescent biomolecules and drugs is presented in extensive tables, treating amino acids, peptides, proteins, bioactive compounds, flavins, pharmaceuticals and also single cell analysis. The tables provide information on analyte nature, sample matrix, optical detection aspects, CE mode and limits of detection. A selection of descriptive applications is discussed in detail to illustrate the potential of native fluorescence detection in CE. It is concluded that CE-Flu is a powerful tool for biomolecular and pharmaceutical analysis, and provides good opportunities for use in lab-on-chip devices.

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Publisher Full Text

Authors+Show Affiliations

de Kort BJ
Department of Biomolecular Analysis, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands. bjdekort@gmail.com
de Jong GJ
No affiliation info available
Somsen GW
No affiliation info available

MeSH

Electrophoresis, CapillaryEquipment DesignLab-On-A-Chip DevicesPeptidesPharmaceutical PreparationsProteinsSingle-Cell AnalysisSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

23427797

Citation

de Kort, Bregje J., et al. "Native Fluorescence Detection of Biomolecular and Pharmaceutical Compounds in Capillary Electrophoresis: Detector Designs, Performance and Applications: a Review." Analytica Chimica Acta, vol. 766, 2013, pp. 13-33.
de Kort BJ, de Jong GJ, Somsen GW. Native fluorescence detection of biomolecular and pharmaceutical compounds in capillary electrophoresis: detector designs, performance and applications: a review. Anal Chim Acta. 2013;766:13-33.
de Kort, B. J., de Jong, G. J., & Somsen, G. W. (2013). Native fluorescence detection of biomolecular and pharmaceutical compounds in capillary electrophoresis: detector designs, performance and applications: a review. Analytica Chimica Acta, 766, 13-33. https://doi.org/10.1016/j.aca.2012.12.006
de Kort BJ, de Jong GJ, Somsen GW. Native Fluorescence Detection of Biomolecular and Pharmaceutical Compounds in Capillary Electrophoresis: Detector Designs, Performance and Applications: a Review. Anal Chim Acta. 2013 Mar 5;766:13-33. PubMed PMID: 23427797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Native fluorescence detection of biomolecular and pharmaceutical compounds in capillary electrophoresis: detector designs, performance and applications: a review. AU - de Kort,Bregje J, AU - de Jong,Gerhardus J, AU - Somsen,Govert W, Y1 - 2012/12/13/ PY - 2012/06/09/received PY - 2012/12/01/revised PY - 2012/12/03/accepted PY - 2013/2/23/entrez PY - 2013/2/23/pubmed PY - 2013/8/24/medline SP - 13 EP - 33 JF - Analytica chimica acta JO - Anal Chim Acta VL - 766 N2 - This review treats the coupling of capillary electrophoresis (CE) with fluorescence detection (Flu) for the analysis of natively fluorescent biomolecular and pharmaceutical compounds. CE-Flu combines the excellent separation efficiency of CE with the high selectivity and sensitivity of Flu. In CE-Flu, an appropriate design of the fluorescence detection cell is very important in order to achieve efficient analyte excitation in and emission light collection from the small cylindrically-shaped detection volume. Therefore, due attention is paid to the various optical detection designs used for CE-Flu, including the applied excitation sources and emission light detectors. Special attention is devoted to wavelength-resolved Flu and to sensitivity issues. Furthermore, he specific requirements for fluorescence detection in microfluidic systems (i.e. chip-based electrophoresis) are discussed. Subsequently, an overview of described applications of CE-Flu for the analysis of natively fluorescent biomolecules and drugs is presented in extensive tables, treating amino acids, peptides, proteins, bioactive compounds, flavins, pharmaceuticals and also single cell analysis. The tables provide information on analyte nature, sample matrix, optical detection aspects, CE mode and limits of detection. A selection of descriptive applications is discussed in detail to illustrate the potential of native fluorescence detection in CE. It is concluded that CE-Flu is a powerful tool for biomolecular and pharmaceutical analysis, and provides good opportunities for use in lab-on-chip devices. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/23427797/Native_fluorescence_detection_of_biomolecular_and_pharmaceutical_compounds_in_capillary_electrophoresis:_detector_designs_performance_and_applications:_a_review_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(12)01771-0 DB - PRIME DP - Unbound Medicine ER -